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product summary
company name :
Addgene
product type :
cDNA
product name :
pAC-aZEA
catalog :
53286
citations: 1
| Reference |
|---|
Cunningham F, Gantt E. A portfolio of plasmids for identification and analysis of carotenoid pathway enzymes: Adonis aestivalis as a case study. Photosynth Res. 2007;92:245-59 pubmed
|
product information
Catalog Number :
53286
Product Name :
pAC-aZEA
article :
| doi | 10.1007/s11120-007-9210-0 |
| id | 8302 |
| pubmed_id | 17634749 |
bacterial resistance :
Chloramphenicol
cloning :
| backbone | pAC-NEUR | ||
| backbone_mutation | An Arabidopsis thaliana cDNA encoding the lycopene epsilon-cyclase enzyme was excised from plasmid pY2F, together with most of the lac promoter, as an AseI-PvuII fragment of ca. 2.3 kB, and ligated in pAC-NEUR that had been digested with HindIII, blunted with the Klenow enzyme and then digested with AseI. | ||
| backbone_origin | Francis X. Cunningham, Jr. | ||
| backbone_size | 11849 | ||
| promoter | |||
| sequencing_primer_3 | |||
| sequencing_primer_5 | |||
| vector_types |
|
growth notes :
For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and amplify for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
growth strain :
Contains crtE, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, crtI gene of Rhodobacter capsulatus, and lcyE cDNA of Arabidopsis thaliana and produces alpha-zeacarotene in E. coli
growth temp :
Grow liquid cultures on a platform shaker at 28 degrees Celsius in darkness for 2-3 days for best alpha-zeacarotene production, or grow on agar plates at room temperature for 3-7 days.
origin :
30
pi :
|
resistance markers :
| 1973 |
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments