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product summary
company name :
Addgene
product type :
cDNA
product name :
pY2F
catalog :
53285
citations: 1
| Reference |
|---|
Cunningham F, Gantt E. A portfolio of plasmids for identification and analysis of carotenoid pathway enzymes: Adonis aestivalis as a case study. Photosynth Res. 2007;92:245-59 pubmed
|
product information
Catalog Number :
53285
Product Name :
pY2F
article :
| doi | 10.1007/s11120-007-9210-0 |
| id | 8302 |
| pubmed_id | 17634749 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pBluescript SK- | |
| backbone_mutation | A cDNA library plasmid referred to as y2 [Cunningham,F.X. Jr., Pogson,B., Sun,Z., McDonald,K.A., DellaPenna,D. and Gantt,E. (1996) Functional analysis of the beta and epsilon lycopene cyclase enzymes of Arabidopsis reveals a mechanism for control of cyclic carotenoid formation. Plant Cell 8, 1613-1626.] contained an Arabidopsis thaliana lcyE cDNA in the reverse orientation relative to the lac promoter of the cloning vector (pBluescript SK-). To improve expression, the cDNA was excised from plasmid y2 as an XhoI-SmaI fragment of 1.9 kB and ligated in pBluescript SK- that had been digested with XhoI, blunted with the Klenow enzyme and then digested with SalI. The resulting plasmid contained the cDNA in the forward orientation relative to the lac promoter. | |
| backbone_origin | Stratagene | |
| backbone_size | 2958 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth strain :
Contains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.
growth temp :
When using together with plasmid pAC-BETA or pAC-BETAipi to produce alpha-carotene, grow liquid cultures at 28 degrees Celsius in darkness for 2-3 days , or grow on agar plates at room temperature for 3-7 days.
origin :
37
pi :
|
resistance markers :
| 1973 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments