mCit-Snap25b D179K A199R | Addgene 53259 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

mCit-Snap25b D179K A199R

catalog :

53259

citations: 1

Reference
Degtyar V, Hafez I, Bray C, Zucker R. Dance of the SNAREs: assembly and rearrangements detected with FRET at neuronal synapses. J Neurosci. 2013;33:5507-23 pubmed publisher

product information

Catalog Number :

53259

Product Name :

mCit-Snap25b D179K A199R

article :

doi	10.1523/JNEUROSCI.2337-12.2013
id	8245
pubmed_id	23536066

bacterial resistance :

Kanamycin

cloning :

backbone

pECFP-C1

backbone_mutation

backbone_origin

Clontech

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

This SNAP25B double mutant is resistant to both botulinum toxin C1 (A199R) and botulinum toxin E (D179K)

growth strain :

SNAP25B double mutant insensitive to both Botulinum toxin C1 N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.

origin :

pi :

alt_names

synaptosomal-associated protein, 25kDa

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	SV40pA-R
sequencing_primer_5	EGFP-C
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	SNAP-25B, SNAP-25a
gene	Snap25
id	25012

genbank_ids

mutation

D179K and A199R

name

SNAP-25B

shRNA_sequence

size

621

species

10116

Rattus norvegicus