pMyc4ElbLuc. | Addgene 53246 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMyc4ElbLuc.

catalog :

53246

citations: 3

Reference
Ding L, Sun Q, Tan K, Chien W, Mayakonda A, Yeoh A, et al. Mutational Landscape of Pediatric Acute Lymphoblastic Leukemia. Cancer Res. 2017;77:390-400 pubmed publisher
Sun Q, Ding L, Tan K, Chien W, Mayakonda A, Lin D, et al. Ordering of mutations in acute myeloid leukemia with partial tandem duplication of MLL (MLL-PTD). Leukemia. 2017;31:1-10 pubmed publisher
Gupta S, Seth A, Davis R. Transactivation of gene expression by Myc is inhibited by mutation at the phosphorylation sites Thr-58 and Ser-62. Proc Natl Acad Sci U S A. 1993;90:3216-20 pubmed

product information

Catalog Number :

53246

Product Name :

pMyc4ElbLuc.

article :

doi
id	8292
pubmed_id	8386367

bacterial resistance :

Ampicillin

cloning :

backbone	pG5ElbLuc
backbone_mutation	The plasmid pG5ElbLuc was prepared from pG5E1bCAT (23) by replacing the chloramphenicol acetyltransferase gene (EcoRI and Sty I fragment) with the firefly luciferase gene (from pGEM-Luc) as a 1745-bp HindIII-StuI blunt-end fragment. The GAL4 binding sites were removed from pG5ElbLuc as a Pst I and Kpn I fragment and replaced with a double-stranded 20-mer oligonucleotide that contains the Elb promoter TATA element (5'-GAGGGTATATAATGTGGTAC- 3' and 5'-CACATTATATACCCTCTGCA-3') to create the plasmid pMycOElbLuc.
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types

growth strain :

reporter plasmid containing 4 myc cDNA binding sites

origin :

pi :

alt_names

cloning

entrez_gene

aliases	MRTL, MYCC, bHLHe39, c-Myc
gene	MYC
id	4609

genbank_ids

mutation

To prepare plasmids with one, two, three, and four Myc binding sites (underlined), a 26-mer double-stranded oligonucleotide (5'-AGCTTAACTGACCACGTGGTCAACTA- 3' and 5'-AGCTTAGTTGACCACGTGGTCAGTTA- 3') was employed in a ligation reaction to obtain the plasmids pMyc4ElbLuc,

name

pMyc4ElbLuc

shRNA_sequence

size

species

9606

Homo sapiens

32644

Other