pMyc2ElbLuc, | Addgene 53244 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMyc2ElbLuc,

catalog :

53244

citations: 1

Reference
Gupta S, Seth A, Davis R. Transactivation of gene expression by Myc is inhibited by mutation at the phosphorylation sites Thr-58 and Ser-62. Proc Natl Acad Sci U S A. 1993;90:3216-20 pubmed

product information

Catalog Number :

53244

Product Name :

pMyc2ElbLuc,

article :

doi
id	8292
pubmed_id	8386367

bacterial resistance :

Ampicillin

cloning :

backbone	pG5ElbLuc
backbone_mutation	The plasmid pG5ElbLuc was prepared from pG5E1bCAT (23) by replacing the chloramphenicol acetyltransferase gene (EcoRI and Sty I fragment) with the firefly luciferase gene (from pGEM-Luc) as a 1745-bp HindIII-StuI blunt-end fragment. The GAL4 binding sites were removed from pG5ElbLuc as a Pst I and Kpn I fragment and replaced with a double-stranded 20-mer oligonucleotide that contains the Elb promoter TATA element (5'-GAGGGTATATAATGTGGTAC- 3' and 5'-CACATTATATACCCTCTGCA-3') to create the plasmid pMycOElbLuc.
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types

growth strain :

reporter plasmid containing 2 myc cDNA binding sites

origin :

pi :

alt_names

cloning

entrez_gene

aliases	MRTL, MYCC, bHLHe39, c-Myc
gene	MYC
id	4609

genbank_ids

mutation

To prepare plasmids with one, two, three, and four Myc binding sites (underlined), a 26-mer double-stranded oligonucleotide (5'-AGCTTAACTGACCACGTGGTCAACTA- 3' and 5'-AGCTTAGTTGACCACGTGGTCAGTTA- 3') was employed in a ligation reaction to obtain the plasmids pMyc2ElbLuc,

name

pMyc2ElbLuc

shRNA_sequence

size

species

9606

Homo sapiens