Stx1a-12aa-mCer | Addgene 53236 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Stx1a-12aa-mCer

catalog :

53236

citations: 1

Reference
Degtyar V, Hafez I, Bray C, Zucker R. Dance of the SNAREs: assembly and rearrangements detected with FRET at neuronal synapses. J Neurosci. 2013;33:5507-23 pubmed publisher

product information

Catalog Number :

53236

Product Name :

Stx1a-12aa-mCer

article :

doi	10.1523/JNEUROSCI.2337-12.2013
id	8245
pubmed_id	23536066

bacterial resistance :

Kanamycin

cloning :

backbone

pECFP-N1

backbone_mutation

This vector was created by substituting ECFP in the original vector by mCerulean. Compared to ECFP, Cerulean contains mutations: S72A, Y145A and H148D. mCerulean also contains A206K mutation to create a monomeric form of the fluorescent protein.

backbone_origin

Clontech

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.

origin :

pi :

alt_names

Stx1a

cloning

entrez_gene

aliases
gene	Stx1a
id	116470

genbank_ids

NM_053788

mutation

name

Syntaxin 1A

shRNA_sequence

size

867

species

10116

Rattus norvegicus