mCit-4aa-SNAP25B | Addgene 53235 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

mCit-4aa-SNAP25B

catalog :

53235

citations: 4

Reference
Sharda N, Pengo T, Wang Z, Kandimalla K. Amyloid-β Peptides Disrupt Interactions Between VAMP-2 and SNAP-25 in Neuronal Cells as Determined by FRET/FLIM. J Alzheimers Dis. 2020;77:423-435 pubmed publisher
Rana M, Kumar P, Lee C, Verardi R, Rajashankar K, Banerjee A. Fatty acyl recognition and transfer by an integral membrane S-acyltransferase. Science. 2018;359: pubmed publisher
Verardi R, Kim J, Ghirlando R, Banerjee A. Structural Basis for Substrate Recognition by the Ankyrin Repeat Domain of Human DHHC17 Palmitoyltransferase. Structure. 2017;25:1337-1347.e6 pubmed publisher
Degtyar V, Hafez I, Bray C, Zucker R. Dance of the SNAREs: assembly and rearrangements detected with FRET at neuronal synapses. J Neurosci. 2013;33:5507-23 pubmed publisher

product information

Catalog Number :

53235

Product Name :

mCit-4aa-SNAP25B

article :

doi	10.1523/JNEUROSCI.2337-12.2013
id	8245
pubmed_id	23536066

bacterial resistance :

Kanamycin

cloning :

backbone

pECFP-C1

backbone_mutation

ECFP has been substituted by citrine, which was further made monomeric by introducing the A206K mutation using the Stratagene QuikChange Site-Directed Mutagenesis Kit, and exchanged with EYFP by digestion with restriction endonucleases after ligation.

backbone_origin

Clontech

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Fluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.

origin :

pi :

alt_names

synaptosomal-associated protein, 25kDa

cloning

entrez_gene

aliases	SNAP-25B, SNAP-25a
gene	Snap25
id	25012

genbank_ids

AB003992

AB003992.1

mutation

name

SNAP-25B

shRNA_sequence

size

621

species

10116

Rattus norvegicus