mCer-4aa-VAMP2 | Addgene 53233 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

mCer-4aa-VAMP2

catalog :

53233

citations: 2

Reference
Sharda N, Pengo T, Wang Z, Kandimalla K. Amyloid-β Peptides Disrupt Interactions Between VAMP-2 and SNAP-25 in Neuronal Cells as Determined by FRET/FLIM. J Alzheimers Dis. 2020;77:423-435 pubmed publisher
Degtyar V, Hafez I, Bray C, Zucker R. Dance of the SNAREs: assembly and rearrangements detected with FRET at neuronal synapses. J Neurosci. 2013;33:5507-23 pubmed publisher

product information

Catalog Number :

53233

Product Name :

mCer-4aa-VAMP2

article :

doi	10.1523/JNEUROSCI.2337-12.2013
id	8245
pubmed_id	23536066

bacterial resistance :

Kanamycin

cloning :

backbone

pECFP-C1

backbone_mutation

This vector was created by mutagenesis of pECFP-C1. Compared to ECFP, Cerulean contains mutations: S72A, Y145A and H148D. mCerulean also contains A206K mutation to create a monomeric form of the fluorescent protein. See more details in Addgene's plasmid 27796.

backbone_origin

Clontech

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.

origin :

pi :

alt_names

Vesicle-Associated Membrane Protein 2

synaptobrevin 2

cloning

entrez_gene

aliases	RATVAMPB, RATVAMPIR, SYB, Syb2
gene	Vamp2
id	24803

genbank_ids

NM_012663

mutation

name

VAMP2

shRNA_sequence

size

351

species

10116

Rattus norvegicus