pBJ FLAG-hPar1 | Addgene 53226 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBJ FLAG-hPar1

catalog :

53226

citations: 3

Reference
Pan C, Maeso D xed az R, Lewis T, Xiang K, Tan L, Liang Y, et al. Antagonizing the irreversible thrombomodulin-initiated proteolytic signaling alleviates age-related liver fibrosis via senescent cell killing. Cell Res. 2023;33:516-532 pubmed publisher
Gong H, An S, Sassmann A, Liu M, Mastej V, Mittal M, et al. PAR1 Scaffolds TGF?RII to Downregulate TGF-? Signaling and Activate ESC Differentiation to Endothelial Cells. Stem Cell Reports. 2016;7:1050-1058 pubmed publisher
Ishii K, Hein L, Kobilka B, Coughlin S. Kinetics of thrombin receptor cleavage on intact cells. Relation to signaling. J Biol Chem. 1993;268:9780-6 pubmed

product information

Catalog Number :

53226

Product Name :

pBJ FLAG-hPar1

article :

doi
id	1091
pubmed_id	7683662

bacterial resistance :

Ampicillin

cloning :

backbone

pBJ1

backbone_mutation

backbone_origin

Mark Davis lab

backbone_size

3300

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The gene for hThrombin Receptor with Flag epitope at its amino terminus. The gene was cloned into the SalI site of pBluescript and excised and inserted into pBJ.

growth strain :

mammalian expression of human Par1 with N-terminal FLAG tag

origin :

pi :

alt_names

coagulation factor II (thrombin) receptor

thrombin receptor

cloning

clone_method	Restriction Enzyme
cloning_site_3	unknown
cloning_site_5	unknown
promoter	SV40
sequencing_primer_3	EBV-rev
sequencing_primer_5	SV40pro F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CF2R, HTR, PAR-1, PAR1, TR
gene	F2R
id	2149

genbank_ids

mutation

Deletion of aa1-39 (native signal peptide)

name

Par1

shRNA_sequence

size

3200

species

9606

Homo sapiens