This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PCRT7 tetR pLtetO MBP-MEK1 XX Amp
catalog :
53225
citations: 1
Reference
Gassaway B, Cardone R, Padyana A, Petersen M, Judd E, Hayes S, et al. Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production. Cell Rep. 2019;29:3394-3404.e9 pubmed publisher
product information
Catalog Number :
53225
Product Name :
PCRT7 tetR pLtetO MBP-MEK1 XX Amp
article :
doi
id8287
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepCRT7
backbone_mutation
backbone_originRinehart lab, Addgene plasmid 52053
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Other
phosphoprotein synthesis
growth strain :
human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis
origin :
37
pi :
alt_names
mitogen-activated protein kinase kinase 1
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterpLtetO
sequencing_primer_3T7 terminal
sequencing_primer_5MBP-F GATGAAGCCCTGAAAGACGCGCAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1
geneMAP2K1
id5604
genbank_ids
mutationCodon optimized for E.coli, both codons for Ser218 and Ser222 were changed to amber codons
nameMEK1
shRNA_sequence
size1198
species
9606
Homo sapiens
tags
locationN terminal on insert
tagMBP
locationC terminal on insert
tagHIS
resistance markers :
1137
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA