This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBABEpuro-HA-ERK2 GOF
catalog :
53203
citations: 5
Reference
Clayton N, Carter E, Fearon A, Heward J, Rodr xed guez Fern xe1 ndez L, Boughetane L, et al. HDAC Inhibition Restores Response to HER2-Targeted Therapy in Breast Cancer via PHLDA1 Induction. Int J Mol Sci. 2023;24: pubmed publisher
Tripathi R, Liu Z, Jain A, Lyon A, Meeks C, Richards D, et al. Combating acquired resistance to MAPK inhibitors in melanoma by targeting Abl1/2-mediated reactivation of MEK/ERK/MYC signaling. Nat Commun. 2020;11:5463 pubmed publisher
Tsang T, Posimo J, Gudiel A, Cicchini M, Feldser D, Brady D. Copper is an essential regulator of the autophagic kinases ULK1/2 to drive lung adenocarcinoma. Nat Cell Biol. 2020;22:412-424 pubmed publisher
Yue J, Lai F, Beckedorff F, Zhang A, Pastori C, Shiekhattar R. Integrator orchestrates RAS/ERK1/2 signaling transcriptional programs. Genes Dev. 2017;31:1809-1820 pubmed publisher
Brady D, Crowe M, Turski M, Hobbs G, Yao X, Chaikuad A, et al. Copper is required for oncogenic BRAF signalling and tumorigenesis. Nature. 2014;509:492-6 pubmed publisher
product information
Catalog Number :
53203
Product Name :
pBABEpuro-HA-ERK2 GOF
article :
doi10.1038/nature13180
id8270
pubmed_id24717435
bacterial resistance :
Ampicillin
cloning :
backbonepBABE puro
backbone_mutation
backbone_origin
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
The sequence discrepancies found during the QC process have no functional consequence.
growth strain :
Expresses HA tagged human ERK2 R67S D321N from puromycin resistance retroviral vector
origin :
37
pi :
alt_names
Extracellular signal regulated kinase 2
MAPK2
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5XhoI
promoter
sequencing_primer_3ACCCTAACTGACACACATTCC
sequencing_primer_5CTTTATCCAGCCCTCAC
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesERK, ERK-2, ERK2, ERT1, MAPK2, NS13, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK
geneMAPK1
id5594
genbank_ids
NM_138957.2
mutationR67S/D321N
nameERK2
shRNA_sequence
size1082
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
plasmid copy :
Addgene Plasmid 23498: pDONR223-MAPK1
resistance markers :
95
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA