This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX4T3-HA-ERK2 D321N
catalog :
53202
citations: 1
Reference
Brady D, Crowe M, Turski M, Hobbs G, Yao X, Chaikuad A, et al. Copper is required for oncogenic BRAF signalling and tumorigenesis. Nature. 2014;509:492-6 pubmed publisher
product information
Catalog Number :
53202
Product Name :
pGEX4T3-HA-ERK2 D321N
article :
doi10.1038/nature13180
id8270
pubmed_id24717435
bacterial resistance :
Ampicillin
cloning :
backbonepGEX4T3
backbone_mutation
backbone_originGE Healthcare
backbone_size4968
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
The sequence discrepancies found during the QC process have no functional consequence.
growth strain :
Expresses GST and HA tagged human ERK2 D321N from bacterial expression vector
origin :
37
pi :
alt_names
Extracellular signal regulated kinase 2
MAPK2
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5XhoI
promoter
sequencing_primer_3CCGGGAGCTGCATGTGTCAGAGG
sequencing_primer_5GGGCTGGCAAGCCACGTTTGGTG
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesERK, ERK-2, ERK2, ERT1, MAPK2, NS13, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK
geneMAPK1
id5594
genbank_ids
NM_138957.2
mutationD321N
nameERK2
shRNA_sequence
size1082
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST
locationN terminal on insert
tagHA
plasmid copy :
Addgene Plasmid 23498: pDONR223-MAPK1
resistance markers :
95
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA