pBABEpuro-HA-MEK1 | Addgene 53195 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBABEpuro-HA-MEK1

catalog :

53195

citations: 3

Reference
Xue P, Zeng F, Duan Q, Xiao J, Liu L, Yuan P, et al. BCKDK of BCAA Catabolism Cross-talking With the MAPK Pathway Promotes Tumorigenesis of Colorectal Cancer. EBioMedicine. 2017;20:50-60 pubmed publisher
Lee L, Gasilina A, Roychoudhury J, Clark J, McCormack F, Pressey J, et al. Real-time genomic profiling of histiocytoses identifies early-kinase domain BRAF alterations while improving treatment outcomes. JCI Insight. 2017;2:e89473 pubmed publisher
Brady D, Crowe M, Turski M, Hobbs G, Yao X, Chaikuad A, et al. Copper is required for oncogenic BRAF signalling and tumorigenesis. Nature. 2014;509:492-6 pubmed publisher

product information

Catalog Number :

53195

Product Name :

pBABEpuro-HA-MEK1

article :

doi	10.1038/nature13180
id	8270
pubmed_id	24717435

bacterial resistance :

Ampicillin

cloning :

backbone

pBABE puro

backbone_mutation

backbone_origin

backbone_size

5169

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth strain :

Expresses HA tagged human MEK1 from puromycin resistance retroviral vector

origin :

pi :

alt_names

MAP2K1

MAPK/ERK kinase 1

Dual specificity mitogen activated protein kinase kinase 1

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5	BamHI
promoter
sequencing_primer_3	ACCCTAACTGACACACATTCC
sequencing_primer_5	CTTTATCCAGCCCTCAC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1
gene	MAP2K1
id	5604

genbank_ids

NM_002755.3

NP_002746.1

mutation

name

MEK1

shRNA_sequence

size

1181

species

9606

Homo sapiens