This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBABEpuro-HA-MEK1-MEK5DD
catalog :
53176
citations: 1
Reference
Brady D, Crowe M, Turski M, Hobbs G, Yao X, Chaikuad A, et al. Copper is required for oncogenic BRAF signalling and tumorigenesis. Nature. 2014;509:492-6 pubmed publisher
product information
Catalog Number :
53176
Product Name :
pBABEpuro-HA-MEK1-MEK5DD
article :
doi10.1038/nature13180
id8270
pubmed_id24717435
bacterial resistance :
Ampicillin
cloning :
backbonepBABE puro
backbone_mutation
backbone_origin
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
The sequence discrepancies found during the QC process have no functional consequence.
growth strain :
Expresses HA tagged chimeric human MEK1 (nucleotides 1 201)-MEK5 (498 1344 with S311DT315D) from puromycin resistance retroviral vector
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoter
sequencing_primer_3ACCCTAACTGACACACATTCC
sequencing_primer_5CTTTATCCAGCCCTCAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1
geneMAP2K1
id5604
genbank_ids
NM_002755.3
NM_145160.1
mutationMEK1 (1-201) MEK5(498 1344 with S311D/T315D)
nameMEK1 (1-201)-MEK5 (498 1344) fusion
shRNA_sequence
size1047
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
plasmid copy :
Addgene Plasmid 21208: W1 and Addgene Plasmid 20514: pWZL Neo Myr Flag MAP2K5
resistance markers :
95
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA