This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pWZLblasti-HA-ERK5
catalog :
53175
citations: 2
Reference
Vaseva A, Blake D, Gilbert T, Ng S, Hostetter G, Azam S, et al. KRAS Suppression-Induced Degradation of MYC Is Antagonized by a MEK5-ERK5 Compensatory Mechanism. Cancer Cell. 2018;34:807-822.e7 pubmed publisher
Brady D, Crowe M, Turski M, Hobbs G, Yao X, Chaikuad A, et al. Copper is required for oncogenic BRAF signalling and tumorigenesis. Nature. 2014;509:492-6 pubmed publisher
product information
Catalog Number :
53175
Product Name :
pWZLblasti-HA-ERK5
article :
doi10.1038/nature13180
id8270
pubmed_id24717435
bacterial resistance :
Ampicillin
cloning :
backbonepWZLblasti
backbone_mutation
backbone_origin
backbone_size5100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
The E310G mutation found during the QC process has no functional consequence.
growth strain :
Expresses HA tagged human ERK5 from blasticidin resistance retroviral vector
origin :
37
pi :
alt_names
Extracellular signal regulated kinase 5
Mitogen activated protein kinase 7
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoter
sequencing_primer_3TTCCGGGCCCTCACATTG
sequencing_primer_5GAACCTCCTCGTTCGACC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBMK1, ERK4, ERK5, PRKM7
geneMAPK7
id5598
genbank_ids
NM_139033.2
mutation
nameERK5
shRNA_sequence
size2450
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
plasmid copy :
Addgene Plasmid 20527: pWZL Neo Myr Flag MAPK7
resistance markers :
95
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA