This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBABEbleo-Flag-BRAFV600E
catalog :
53156
citations: 7
Reference
Tsang T, Gu X, Davis C, Posimo J, Miller Z, Brady D. BRAFV600E-Driven Lung Adenocarcinoma Requires Copper to Sustain Autophagic Signaling and Processing. Mol Cancer Res. 2022;20:1096-1107 pubmed publisher
Traversi F, Stooss A, Dettmer M, Charles R. BRAFV600E Overrides NOTCH Signaling in Thyroid Cancer. Thyroid. 2020;: pubmed publisher
Schwartz J, Ma J, Lamprecht T, Walsh M, Wang S, Bryant V, et al. The genomic landscape of pediatric myelodysplastic syndromes. Nat Commun. 2017;8:1557 pubmed publisher
Welsch M, Kaplan A, Chambers J, Stokes M, Bos P, Zask A, et al. Multivalent Small-Molecule Pan-RAS Inhibitors. Cell. 2017;168:878-889.e29 pubmed publisher
Dela Cruz F, Diolaiti D, Turk A, Rainey A, Ambesi Impiombato A, Andrews S, et al. A case study of an integrative genomic and experimental therapeutic approach for rare tumors: identification of vulnerabilities in a pediatric poorly differentiated carcinoma. Genome Med. 2016;8:116 pubmed publisher
Zhang P, Feng S, Liu G, Wang H, Zhu H, Ren Q, et al. Mutant B-Raf(V600E) Promotes Melanoma Paracellular Transmigration by Inducing Thrombin-mediated Endothelial Junction Breakdown. J Biol Chem. 2016;291:2087-106 pubmed publisher
Brady D, Crowe M, Turski M, Hobbs G, Yao X, Chaikuad A, et al. Copper is required for oncogenic BRAF signalling and tumorigenesis. Nature. 2014;509:492-6 pubmed publisher
product information
Catalog Number :
53156
Product Name :
pBABEbleo-Flag-BRAFV600E
article :
doi10.1038/nature13180
id8270
pubmed_id24717435
bacterial resistance :
Ampicillin
cloning :
backbonepBABEbleo(zeo)
backbone_mutation
backbone_origin
backbone_size4888
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth strain :
Expresses Flag tagged human oncogenic BRAFV600E from bleomycin resistance retroviral vector
origin :
37
pi :
alt_names
B-Raf proto-oncogene serine/threonine-protein kinase
BRAF1
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5SalI
promoter
sequencing_primer_3ACCCTAACTGACACACATTCC
sequencing_primer_5CTTTATCCAGCCCTCAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesB-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1
geneBRAF
id673
genbank_ids
NM_004333.4
mutationV600E
nameBRAF
shRNA_sequence
size2300
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFLAG
plasmid copy :
Addgene Plasmid 15269: pBabe-Puro-BRAF-V600E
resistance markers :
95
tags :
High Copy
terms :
Zeocin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA