This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET28a-HisYFP-Sp100
catalog :
53141
citations: 1
Reference
Flotho A, Werner A, Winter T, Frank A, Ehret H, Melchior F. Recombinant reconstitution of sumoylation reactions in vitro. Methods Mol Biol. 2012;832:93-110 pubmed publisher
product information
Catalog Number :
53141
Product Name :
pET28a-HisYFP-Sp100
article :
doi10.1007/978-1-61779-474-2_5
id7730
pubmed_id22350878
bacterial resistance :
Kanamycin
cloning :
backbonepET28a
backbone_mutation
backbone_originNovagen
backbone_size5369
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Please note that the plasmid contains some 3'UTR sequence following the CDS that is not found in the full plasmid sequence. This does not have any functional consequence.
growth strain :
E.coli expression of HisYFP tagged human Sp100 fragment
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5EcoRI
promoterT7
sequencing_primer_3T7-term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliaseslysp100b
geneSP100
id6672
genbank_ids
mutationfragment (amino acids 71-480); deletion of L75
nameSp100A fragment
shRNA_sequence
size2028
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHis-YFP
resistance markers :
1939
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA