pGfa(B3)-nLac | Addgene 53132 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGfa(B3)-nLac

catalog :

53132

citations: 1

Reference
de Leeuw B, Su M, ter Horst M, Iwata S, Rodijk M, Hoeben R, et al. Increased glia-specific transgene expression with glial fibrillary acidic protein promoters containing multiple enhancer elements. J Neurosci Res. 2006;83:744-53 pubmed

product information

Catalog Number :

53132

Product Name :

pGfa(B3)-nLac

article :

doi	10.1002/jnr.20776
id	8264
pubmed_id	16496373

bacterial resistance :

Ampicillin

cloning :

backbone

pUC18

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Mouse Targeting

growth notes :

This plasmid is a variant of pGfa2-nLac (Addgene plasmid #53126) constructed by inserting 3 copies of the gfa2 B segment (Besnard et al. 1991) into the Sma I site just upstream of the basal promoter. It has about 75 times higher activity than pGfa2-nLac in transfected cells, but does not work in transgenic mice; possibly the high level of expression it produces is toxic.

growth strain :

expression of nuclear LacZ driven by Gfa2 promoter with 3 additional B sites, has about 75 times higher activity than pGfa2-nLac in transfected cells

origin :

pi :

alt_names

glial fibrillary acidic protein

GFAP

cloning

clone_method	Restriction Enzyme
cloning_site_3	BglII
cloning_site_5	BglII
promoter	human Gfa2
sequencing_primer_3	MB-352 GACGTTGTAAAACGACGGCCAGT
sequencing_primer_5	MB-351 CATCGCCAGTCTAGCCCACTCCT
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ALXDRD
gene	GFAP
id	2670

genbank_ids

mutation

-2163 to +47, with 3 copies of the gfa2 B segment inserted into the Sma I site just upstream of the basal promoter, with the initiating ATG mutated to TTG

name

Gfa2

shRNA_sequence

size

species

9606

Homo sapiens