This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGfaABC1D-nLac
catalog :
53131
citations: 2
Reference
Joshi C, Raghavan V, Vijayaraghavalu S, Gao Y, Saraswathy M, Labhasetwar V, et al. Reaching for the Stars in the Brain: Polymer-Mediated Gene Delivery to Human Astrocytes. Mol Ther Nucleic Acids. 2018;12:645-657 pubmed publisher
Lee Y, Messing A, Su M, Brenner M. GFAP promoter elements required for region-specific and astrocyte-specific expression. Glia. 2008;56:481-93 pubmed publisher
product information
Catalog Number :
53131
Product Name :
pGfaABC1D-nLac
article :
doi10.1002/glia.20622
id8263
pubmed_id18240313
bacterial resistance :
Ampicillin
cloning :
backbonepUC18
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Mouse Targeting
growth notes :
This plasmid is similar to pGfa28-nlac with the addition of bp -1488 to -1256 (the first part of the C region).
growth strain :
expression of lacZ driven by human GfaABC1D promoter
origin :
37
pi :
alt_names
glial fibrillary acidic protein
GFAP
cloning
clone_methodRestriction Enzyme
cloning_site_3BglII
cloning_site_5BglII
promoterhuman Gfa2
sequencing_primer_3MB-352 GACGTTGTAAAACGACGGCCAGT
sequencing_primer_5MB-351 CATCGCCAGTCTAGCCCACTCCT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALXDRD
geneGFAP
id2670
genbank_ids
mutationcontains ABC1D regions of Gfa2 promoter, -1757 to -1256 and -132 to +47 with ATG to TGT mutation at +15
nameGfa2
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagNLS
locationC terminal on insert
tagLacZ
locationC terminal on insert
tagmP1
resistance markers :
1935
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA