pGfa28-nLac | Addgene 53130 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGfa28-nLac

catalog :

53130

citations: 2

Reference
Joshi C, Raghavan V, Vijayaraghavalu S, Gao Y, Saraswathy M, Labhasetwar V, et al. Reaching for the Stars in the Brain: Polymer-Mediated Gene Delivery to Human Astrocytes. Mol Ther Nucleic Acids. 2018;12:645-657 pubmed publisher
Lee Y, Su M, Messing A, Brenner M. Astrocyte heterogeneity revealed by expression of a GFAP-LacZ transgene. Glia. 2006;53:677-87 pubmed

product information

Catalog Number :

53130

Product Name :

pGfa28-nLac

article :

doi	10.1002/glia.20320
id	8262
pubmed_id	16482522

bacterial resistance :

Ampicillin

cloning :

backbone

pUC18

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Mouse Targeting

growth notes :

This plasmid is identical to pGfa2-nLac (Addgene plasmid #53126), except that the gfa2 sequences between -2163 to -1758 and between -1488 to -133 have been deleted.

growth strain :

Expression of LacZ driven by partial human gfa2 promoter, expression is localized to a subset of astrocytes in mice.

origin :

pi :

alt_names

glial fibrillary acidic protein

GFAP

cloning

clone_method	Restriction Enzyme
cloning_site_3	BglII
cloning_site_5	BglII
promoter	human Gfa2
sequencing_primer_3	MB-352 GACGTTGTAAAACGACGGCCAGT
sequencing_primer_5	MB-351 CATCGCCAGTCTAGCCCACTCCT
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ALXDRD
gene	GFAP
id	2670

genbank_ids

mutation

contains the A and B regions (bp -1757 to -1489) joined to the D region (-132 to -57) and the basal promoter (-56 to +47) with the initiating ATG mutated to TTG

name

Gfa2

shRNA_sequence

size

species

9606

Homo sapiens