This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGfa2-EGFP
catalog :
53128
product information
Catalog Number :
53128
Product Name :
pGfa2-EGFP
article :
doi
id8261
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepGfa2-cLac
backbone_mutation
backbone_originBrenner Lab
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Mouse Targeting
growth notes :
This plasmid was made by digesting pGfa2-cLac with BamHI to remove the lacZ gene (digestion of pGfa2-nLac would produce the same recipient), blunt ending by filling in, and inserting the SmaI/StuI fragment from Clontech's pEGFP that contains the EGFP coding region. The first two bases from each of the filled in BamHI sites were lost during the construction.
growth strain :
Expression of EGFP in mouse astrocytes
origin :
37
pi :
alt_names
glial fibrillary acidic protein
GFAP
cloning
clone_methodRestriction Enzyme
cloning_site_3BglII
cloning_site_5BglII
promoterhuman Gfa2
sequencing_primer_3MB-352 GACGTTGTAAAACGACGGCCAGT
sequencing_primer_5MB-351 CATCGCCAGTCTAGCCCACTCCT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALXDRD
geneGFAP
id2670
genbank_ids
mutationcontains -2163 to +47 (the human gfa2 segment), with the initiating ATG mutated to TTG
nameGfa2
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagEGFP
locationC terminal on insert
tagmP1
resistance markers :
1935
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA