pGfa2-nLac | Addgene 53126 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGfa2-nLac

catalog :

53126

citations: 5

Reference
Men Y, Ye L, Risgaard R, Promes V, Zhao X, Paukert M, et al. Astroglial FMRP deficiency cell-autonomously up-regulates miR-128 and disrupts developmental astroglial mGluR5 signaling. Proc Natl Acad Sci U S A. 2020;117:25092-25103 pubmed publisher
Men Y, Yelick J, Jin S, Tian Y, Chiang M, Higashimori H, et al. Exosome reporter mice reveal the involvement of exosomes in mediating neuron to astroglia communication in the CNS. Nat Commun. 2019;10:4136 pubmed publisher
Brenner M, Messing A, Olsen M. AP-1 and the injury response of the GFAP gene. J Neurosci Res. 2019;97:149-161 pubmed publisher
Joshi C, Raghavan V, Vijayaraghavalu S, Gao Y, Saraswathy M, Labhasetwar V, et al. Reaching for the Stars in the Brain: Polymer-Mediated Gene Delivery to Human Astrocytes. Mol Ther Nucleic Acids. 2018;12:645-657 pubmed publisher
Brenner M, Kisseberth W, Su Y, Besnard F, Messing A. GFAP promoter directs astrocyte-specific expression in transgenic mice. J Neurosci. 1994;14:1030-7 pubmed

product information

Catalog Number :

53126

Product Name :

pGfa2-nLac

article :

doi
id	8260
pubmed_id	8120611

bacterial resistance :

Ampicillin

cloning :

backbone

pUC18

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Mouse Targeting

growth notes :

The plasmid is a pUC18 vector containing the human GFAP sequences from -2163 to +47 (the human gfa2 segment), with the initiating ATG mutated to TTG, placed in front of the nuclear targeted E. coli lacZ gene, which in turn is followed by a fragment of the mouse protamine-1 gene. The latter supplies an intron, stabilizing 3' UTR, and a polyadenylation signal. If cloning a cDNA, it is advisable to retain the mP-1 segment. The lacZ gene can be excised by digestion with BamHI, and replaced with your gene of interest. If cloning a genomic sequence that includes an intron and polyadenylation site (this may compromise astrocyte specificity--see Su et al. 2004), the mP-1 region can also be excised, or alternatively, a number of sites flank the gfa2 segment allowing its isolation. Restriction sites that may be useful are as follows: EcoRI: flanks the gfa2, lacZ, and mP-1 segments Bgl II: 5' flank of gfa2 & 3' flank of mP-1 Bam HI: flanks the lacZ gene Sal I: cuts uniquely between the gfa2 promoter and the LacZ gene Sph I and Hind III: cut uniquely just 3' of the mP-1 segment Although the GFAP promoter appears to be the best choice for targeting transgene expression to astrocytes in mice, please be aware that neuronal expression has also occasionally been observed (Su et al. 2004).

growth strain :

Expression of LacZ driven by the human Gfa2 promoter. LacZ can also be replaced with the gene of interest for targeting transgene expression to astrocytes in mice.

origin :

pi :

alt_names

glial fibrillary acidic protein

GFAP

cloning

clone_method	Restriction Enzyme
cloning_site_3	BglII
cloning_site_5	BglII
promoter	human GFAP
sequencing_primer_3	MB-352 GACGTTGTAAAACGACGGCCAGT
sequencing_primer_5	MB-351 CATCGCCAGTCTAGCCCACTCCT
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ALXDRD
gene	GFAP
id	2670

genbank_ids

mutation

contains -2163 to +47 (the human gfa2 segment), with the initiating ATG mutated to TTG

name

Gfa2

shRNA_sequence

size

species

9606

Homo sapiens