This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti-OC-IRES-BSD
catalog :
53118
citations: 1
Reference
Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y, et al. High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells. Nature. 2014;509:487-91 pubmed publisher
product information
Catalog Number :
53118
Product Name :
pLenti-OC-IRES-BSD
article :
doi10.1038/nature13166
id8257
pubmed_id24717434
bacterial resistance :
Ampicillin
cloning :
backbonepLenti-CMV-BSD
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
To create stable cell clones with high-level expression of Cas9 and OCT1
origin :
37
pi :
alt_names
POU class 2 homeobox 1
POU2F1
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesOCT1, OTF1, Oct1Z, oct-1B
genePOU2F1
id5451
genbank_ids
mutation
nameOCT1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagP2A
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3pCDH-rev
sequencing_primer_5unknown
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationhuman codon-optimized
nameCas9
shRNA_sequence
size
species
32644
Other
Streptococcus pyogenes
tags
locationN terminal on insert
tagNLS
locationC terminal on insert
tagNLS
locationC terminal on insert
tagIRES-BSD
resistance markers :
1932
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA