pME18ST-d5-HT1B | Addgene 53108 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pME18ST-d5-HT1B

catalog :

53108

citations: 1

Reference
Gasque G, Conway S, Huang J, Rao Y, Vosshall L. Small molecule drug screening in Drosophila identifies the 5HT2A receptor as a feeding modulation target. Sci Rep. 2013;3:srep02120 pubmed publisher

product information

Catalog Number :

53108

Product Name :

pME18ST-d5-HT1B

article :

doi	10.1038/srep02120
id	8250
pubmed_id	23817146

bacterial resistance :

Ampicillin

cloning :

backbone	pME18ST
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types

growth notes :

Note that this plasmid has a few differences from the Entrez Genbank reference sequence for Drosophila 5-HT1B. It was cloned from transgenic fly that has UAS-d5-HT1B (Bloomington #27632) and can be considered a functional variant. Plasmid can be directly used for expression in HEK cells. Insert cloned into the NotI and XhoI sites. Has a vertebrate Kozak sequence (GCCACC) immediately upstream of the ATG.

growth strain :

Expression of Drosophila 5-HT1B

origin :

pi :

alt_names

Serotonin receptor 1B

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	XhoI
promoter
sequencing_primer_3	SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')
sequencing_primer_5	pcDL-F (5'-GTTGCCTTTACTTCTAGGCCT-3')
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG15113, 5-HT-dro2B, 5-HT1BDro, 5-HT[1B]Dro, 5-HT[[1BDro]], 5-HT[[1B]], 5-HT[[1B]]DRO, 5-HT[[1B]]Dro, 5-HT[[dro2B]], 5HT-R2B, 5HT-dro2B, 5HT-dro[[2B]], 5HT1B, 5htdro2b, CG15113, CG30126, DRO2B, Dm5HTdro2B, Dmel CG15113, Dmel_CG30126, d5-HT1B
gene	5-HT1B
id	37191

genbank_ids

mutation

name

5HT1B

shRNA_sequence

size

species

7227

Drosophila melanogaster