This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
FokI-dCas9
catalog :
52970
citations: 8
Reference
Yamada S, Ko T, Ito M, Sassa T, Nomura S, Okuma H, et al. TEAD1 trapping by the Q353R-Lamin A/C causes dilated cardiomyopathy. Sci Adv. 2023;9:eade7047 pubmed publisher
Jubair L, Fallaha S, McMillan N. Systemic Delivery of CRISPR/Cas9 Targeting HPV Oncogenes Is Effective at Eliminating Established Tumors. Mol Ther. 2019;27:2091-2099 pubmed publisher
Bosshard S, Duroy P, Mermod N. A role for alternative end-joining factors in homologous recombination and genome editing in Chinese hamster ovary cells. DNA Repair (Amst). 2019;82:102691 pubmed publisher
Nottle M, Salvaris E, Fisicaro N, McIlfatrick S, Vassiliev I, Hawthorne W, et al. Targeted insertion of an anti-CD2 monoclonal antibody transgene into the GGTA1 locus in pigs using FokI-dCas9. Sci Rep. 2017;7:8383 pubmed publisher
Pan Y, Shen N, Jung Klawitter S, Betzen C, Hoffmann G, Hoheisel J, et al. CRISPR RNA-guided FokI nucleases repair a PAH variant in a phenylketonuria model. Sci Rep. 2016;6:35794 pubmed publisher
Terao M, Tamano M, Hara S, Kato T, Kinoshita M, Takada S. Utilization of the CRISPR/Cas9 system for the efficient production of mutant mice using crRNA/tracrRNA with Cas9 nickase and FokI-dCas9. Exp Anim. 2016;65:275-83 pubmed publisher
Hara S, Tamano M, Yamashita S, Kato T, Saito T, Sakuma T, et al. Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9. Sci Rep. 2015;5:11221 pubmed publisher
Guilinger J, Thompson D, Liu D. Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification. Nat Biotechnol. 2014;32:577-582 pubmed publisher
product information
Catalog Number :
52970
Product Name :
FokI-dCas9
article :
doi10.1038/nbt.2909
id8604
pubmed_id24770324
bacterial resistance :
Ampicillin
cloning :
backboneJDS246
backbone_mutation
backbone_origin
backbone_size3400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth strain :
Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3CAGGGTCAAGGAAGGCACG
sequencing_primer_5TAATACGACTCACTATAGG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationD10A and H840A in Cas9
nameFok1 fused to dCas9
shRNA_sequence
size4800
species
32644
Other
tags
locationN terminal on insert
tagNLS
locationN terminal on insert
tagFok1 nuclease (homodimeric wildtype)
resistance markers :
774
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA