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product summary
company name :
Addgene
product type :
cDNA
product name :
lentiCas9-Blast
catalog :
52962
citations: 600
Reference
Catalanotto M, Vaz J, Abshire C, Youngblood R, Chu M, Levine H, et al. Dual role of CASP8AP2/FLASH in regulating epithelial-to-mesenchymal transition plasticity (EMP). Transl Oncol. 2024;39:101837 pubmed publisher
Huth T, Dreher E, Lemke S, Fritzsche S, Sugiyanto R, Castven D, et al. Chromosome 8p engineering reveals increased metastatic potential targetable by patient-specific synthetic lethality in liver cancer. Sci Adv. 2023;9:eadh1442 pubmed publisher
Sugimoto E, Li J, Hayashi Y, Iida K, Asada S, Fukushima T, et al. Hyperactive Natural Killer cells in Rag2 knockout mice inhibit the development of acute myeloid leukemia. Commun Biol. 2023;6:1294 pubmed publisher
Seibert M, Koschade S, Stolp V, H xe4 upl B, Wempe F, Serve H, et al. The MYC-Regulated RNA-Binding Proteins hnRNPC and LARP1 Are Drivers of Multiple Myeloma Cell Growth and Disease Progression and Negatively Predict Patient Survival. Cancers (Basel). 2023;15: pubmed publisher
Ramos A, Koch C, Liu Lupo Y, Hellinger R, Kyung T, Abbott K, et al. Leukemia-intrinsic determinants of CAR-T response revealed by iterative in vivo genome-wide CRISPR screening. Nat Commun. 2023;14:8048 pubmed publisher
Maimaitili M, Chen M, Febbraro F, Ucuncu E, Kelly R, Niclis J, et al. Enhanced production of mesencephalic dopaminergic neurons from lineage-restricted human undifferentiated stem cells. Nat Commun. 2023;14:7871 pubmed publisher
Tub xed o Santamar xed a N, Jayavelu A, Schnoeder T, Eifert T, Hsu C, Perner F, et al. Immunoproteasome function maintains oncogenic gene expression in KMT2A-complex driven leukemia. Mol Cancer. 2023;22:196 pubmed publisher
Barroso Gomila O, Merino Cacho L, Muratore V, Pérez C, Taibi V, Maspero E, et al. BioE3 identifies specific substrates of ubiquitin E3 ligases. Nat Commun. 2023;14:7656 pubmed publisher
Hao S, Zhang X, Ning K, Feng Z, Park S, Aksu Kuz C, et al. Identification of host essential factors for recombinant AAV transduction of the polarized human airway epithelium. J Virol. 2023;97:e0133023 pubmed publisher
Kondo N, Utsumi T, Shimizu Y, Takemoto A, Oh hara T, Uchibori K, et al. MIG6 loss confers resistance to ALK/ROS1 inhibitors in NSCLC through EGFR activation by low-dose EGF. JCI Insight. 2023;8: pubmed publisher
Wang Z, Luo M, Liang Q, Zhao K, Hu Y, Wang W, et al. Landscape of enhancer disruption and functional screen in melanoma cells. Genome Biol. 2023;24:248 pubmed publisher
Poohadsuan J, O Doherty G, Owattanapanich W, Kungwankiattichai S, Rojanasakul Y, Issaragrisil S, et al. Cardiac glycoside ouabain efficiently targets leukemic stem cell apoptotic machinery independent of cell differentiation status. Cell Commun Signal. 2023;21:283 pubmed publisher
Pereira R, Kumar R, Cais A, Paulini L, Kahler A, Bravo J, et al. Distinct and targetable role of calcium-sensing receptor in leukaemia. Nat Commun. 2023;14:6242 pubmed publisher
Heyes E, Wilhelmson A, Wenzel A, Manhart G, Eder T, Schuster M, et al. TET2 lesions enhance the aggressiveness of CEBPA-mutant acute myeloid leukemia by rebalancing GATA2 expression. Nat Commun. 2023;14:6185 pubmed publisher
Kramer N, Prakash G, Isaac R, Choquet K, SOTO I, Petrova B, et al. Regulators of mitonuclear balance link mitochondrial metabolism to mtDNA expression. Nat Cell Biol. 2023;25:1575-1589 pubmed publisher
Tan K, Lu W, Chen F, Shi H, Ma Y, Chen Z, et al. CRISPR-Cas9 knockout screening identifies KIAA1429 as an essential gene in Ewing sarcoma. J Exp Clin Cancer Res. 2023;42:250 pubmed publisher
Benabdallah N, Dalal V, Scott R, Marcous F, Sotiriou A, Kommoss F, et al. Aberrant gene activation in synovial sarcoma relies on SSX specificity and increased PRC1.1 stability. Nat Struct Mol Biol. 2023;30:1640-1652 pubmed publisher
Cui Y, Miao Y, Cao L, Guo L, Cui Y, Yan C, et al. Activation of melanocortin-1 receptor signaling in melanoma cells impairs T cell infiltration to dampen antitumor immunity. Nat Commun. 2023;14:5740 pubmed publisher
Lv X, Lu X, Cao J, Luo Q, Ding Y, Peng F, et al. Modulation of the proteostasis network promotes tumor resistance to oncogenic KRAS inhibitors. Science. 2023;381:eabn4180 pubmed publisher
Yousefi M, Lee W, Chan W, He W, Mah M, Yong C, et al. Betacoronaviruses SARS-CoV-2 and HCoV-OC43 infections in IGROV-1 cell line require aryl hydrocarbon receptor. Emerg Microbes Infect. 2023;12:2256416 pubmed publisher
Hussein B, Kristenson L, Pesce S, W xf6 hr A, Tian Y, Hallner A, et al. NKG2A gene variant predicts outcome of immunotherapy in AML and modulates the repertoire and function of NK cells. J Immunother Cancer. 2023;11: pubmed publisher
Zhao Y, Ruan J, Li Z, Su X, Chen K, Lin Y, et al. OTUB1 inhibits breast cancer by non-canonically stabilizing CCN6. Clin Transl Med. 2023;13:e1385 pubmed publisher
Minderman M, Lantermans H, van der Zwaan C, Hoogendijk A, van den Biggelaar M, Kersten M, et al. The oncogenic human B-cell lymphoma MYD88 L265P mutation genocopies activation by phosphorylation at the Toll/interleukin-1 receptor (TIR) domain. Blood Cancer J. 2023;13:125 pubmed publisher
Sklavenitis Pistofidis R, Lightbody E, Reidy M, Tsuji J, Aranha M, Heilpern Mallory D, et al. Systematic characterization of therapeutic vulnerabilities in Multiple Myeloma with Amp1q reveals increased sensitivity to the combination of MCL1 and PI3K inhibitors. bioRxiv. 2023;: pubmed publisher
Iaffaldano B, Marino M, Reiser J. CRISPR library screening to develop HEK293-derived cell lines with improved lentiviral vector titers. Front Genome Ed. 2023;5:1218328 pubmed publisher
Sansbury S, Serebrenik Y, Lapidot T, Burslem G, Shalem O. Pooled tagging and hydrophobic targeting of endogenous proteins for unbiased mapping of unfolded protein responses. bioRxiv. 2023;: pubmed publisher
Li J, Vasciaveo A, Karagiannis D, Sun Z, Chen X, Socciarelli F, et al. NSD2 maintains lineage plasticity and castration-resistance in neuroendocrine prostate cancer. bioRxiv. 2023;: pubmed publisher
Wang J, Liu H, Yang Y, Tan Y, Sun L, Guo Z, et al. Genome-scale CRISPR screen identifies TRIM2 and SLC35A1 associated with porcine epidemic diarrhoea virus infection. Int J Biol Macromol. 2023;:125962 pubmed publisher
Rojas López M, Gil Marqu xe9 s M, Kharbanda V, Zajac A, Miller K, Wood T, et al. NLRP11 is a pattern recognition receptor for bacterial lipopolysaccharide in the cytosol of human macrophages. Sci Immunol. 2023;8:eabo4767 pubmed publisher
Sun T, Xu Y, Xiang Y, Ou J, Soderblom E, Diao Y. Crosstalk between RNA m6A and DNA methylation regulates transposable element chromatin activation and cell fate in human pluripotent stem cells. Nat Genet. 2023;: pubmed publisher
Cusan M, Shen H, Zhang B, Liao A, Yang L, Jin M, et al. SF3B1 mutation and ATM deletion codrive leukemogenesis via centromeric R-loop dysregulation. J Clin Invest. 2023;133: pubmed publisher
Morgens D, Gulyas L, Souza A, Glaunsinger B. Coding and non-coding elements comprise a regulatory network controlling transcription in Kaposi's sarcoma-associated herpesvirus. bioRxiv. 2023;: pubmed publisher
Garcia N, Becerra J, McKinney B, DiMarco A, Wu F, Fitzgibbon M, et al. APOBEC3 activity promotes the survival and evolution of drug-tolerant persister cells during acquired resistance to EGFR inhibitors in lung cancer. bioRxiv. 2023;: pubmed publisher
Chen X, Li Y, Zhu F, Xu X, Estrella B, Pazos M, et al. Context-defined cancer co-dependency mapping identifies a functional interplay between PRC2 and MLL-MEN1 complex in lymphoma. Nat Commun. 2023;14:4259 pubmed publisher
Hansen S, Larsen H, Pikkupeura L, Maciag G, Guiu J, M xfc ller I, et al. An organoid-based CRISPR-Cas9 screen for regulators of intestinal epithelial maturation and cell fate. Sci Adv. 2023;9:eadg4055 pubmed publisher
Kamikawa Y, Wu Z, Nakazawa N, Ito T, Saito A, Imaizumi K. Impact of cell cycle on repair of ruptured nuclear envelope and sensitivity to nuclear envelope stress in glioblastoma. Cell Death Discov. 2023;9:233 pubmed publisher
Lin Z, Liu Y, Xu T, Su T, Yang Y, Liang R, et al. STAT3-Mediated Promoter-Enhancer Interaction Up-Regulates Inhibitor of DNA Binding 1 (ID1) to Promote Colon Cancer Progression. Int J Mol Sci. 2023;24: pubmed publisher
Rafnsdottir S, Jang K, Halldorsdottir S, Tomasdottir A, Vinod M, M xf6 ller K, et al. SMYD5 is a novel epigenetic gatekeeper of the mild hypothermia response. bioRxiv. 2023;: pubmed publisher
Yagi K, Shimada S, Akiyama Y, Hatano M, Asano D, Ishikawa Y, et al. Loss of SFXN1 mitigates lipotoxicity and predicts poor outcome in non-viral hepatocellular carcinoma. Sci Rep. 2023;13:9449 pubmed publisher
Huang B, Zeng Z, Li H, Li Z, Chen X, Guo J, et al. Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids. bioRxiv. 2023;: pubmed publisher
Tsu B, Agarwal R, Gokhale N, Kulsuptrakul J, Ryan A, Fay E, et al. Host-specific sensing of coronaviruses and picornaviruses by the CARD8 inflammasome. PLoS Biol. 2023;21:e3002144 pubmed publisher
Haldrup J, Weiss S, Schmidt L, S xf8 rensen K. Investigation of enzalutamide, docetaxel, and cabazitaxel resistance in the castration resistant prostate cancer cell line C4 using genome-wide CRISPR/Cas9 screening. Sci Rep. 2023;13:9043 pubmed publisher
Rishiq A, Bsoul R, Pick O, Mandelboim O. Studying TIGIT activity against tumors through the generation of knockout mice. Oncoimmunology. 2023;12:2217735 pubmed publisher
Cuesta Casanovas L, Delgado Mart xed nez J, Cornet Masana J, Carb xf3 J, Ban xfa s Mulet A, Guijarro F, et al. Prolactin receptor signaling induces acquisition of chemoresistance and reduces clonogenicity in acute myeloid leukemia. Cancer Cell Int. 2023;23:97 pubmed publisher
Wit N, Gogola E, West J, Vornb xe4 umen T, Seear R, Bailey P, et al. A histone deacetylase 3 and mitochondrial complex I axis regulates toxic formaldehyde production. Sci Adv. 2023;9:eadg2235 pubmed publisher
Zhang H, Yan J, Lu Z, Zhou Y, Zhang Q, Cui T, et al. Deep sampling of gRNA in the human genome and deep-learning-informed prediction of gRNA activities. Cell Discov. 2023;9:48 pubmed publisher
Kim N, Choi S, Kim S, Song M, Seo J, Min S, et al. Deep learning models to predict the editing efficiencies and outcomes of diverse base editors. Nat Biotechnol. 2023;: pubmed publisher
Wang J, Zhang C, Huang Y, Ruan Y, Hu Y, Wang J, et al. Parallel Genome-Wide CRISPR Screens to Identify State-Dependent Self-Renewal Regulators of Mouse Embryonic Stem Cells. Stem Cells Dev. 2023;: pubmed publisher
Hur J, Kim Y, Choi D, Kang D, Kim J, Yoo H, et al. Role of Gasdermin E in the Biogenesis of Apoptotic Cell-Derived Exosomes. J Immunol. 2023;: pubmed publisher
Guo L, Mao Q, He J, Liu X, Piao X, Luo L, et al. Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies. Cell Res. 2023;33:497-515 pubmed publisher
Morris J, Caragine C, Daniloski Z, Domingo J, Barry T, Lu L, et al. Discovery of target genes and pathways at GWAS loci by pooled single-cell CRISPR screens. Science. 2023;380:eadh7699 pubmed publisher
Hur S, Somerville T, Wu X, Maia Silva D, Demerdash O, Tuveson D, et al. p73 activates transcriptional signatures of basal lineage identity and ciliogenesis in pancreatic ductal adenocarcinoma. bioRxiv. 2023;: pubmed publisher
Bishop T, Subramanian C, Bilotta E, Garnar Wortzel L, Ramos A, Zhang Y, et al. Acetyl-CoA biosynthesis drives resistance to histone acetyltransferase inhibition. Nat Chem Biol. 2023;: pubmed publisher
Zorea J, Motro Y, Mazor R, Carmi Y, Shulman Z, Mahajna J, et al. TRAF3 suppression encourages B cell recruitment and prolongs survival of microbiome-intact mice with ovarian cancer. J Exp Clin Cancer Res. 2023;42:107 pubmed publisher
Baro Sastre B, Kim C, Lin C, Kongsomboonvech A, Tetard M, Salinas N, et al. Plasmodium falciparum exploits CD44 as a co-receptor for erythrocyte invasion. bioRxiv. 2023;: pubmed publisher
Li M, Yang L, Chan A, Pokharel S, Liu Q, Mattson N, et al. Epigenetic Control of Translation Checkpoint and Tumor Progression via RUVBL1-EEF1A1 Axis. Adv Sci (Weinh). 2023;10:e2206584 pubmed publisher
McCollum C, Didychuk A, Liu D, Murray Nerger L, Cristea I, Glaunsinger B. The viral packaging motor potentiates Kaposi's sarcoma-associated herpesvirus gene expression late in infection. PLoS Pathog. 2023;19:e1011163 pubmed publisher
Basu A, Champagne R, Patel N, Nicholson E, Weiss R. TFCP2 is a transcriptional regulator of heparan sulfate assembly and melanoma cell growth. J Biol Chem. 2023;299:104713 pubmed publisher
Ruan Y, Wang J, Yu M, Wang F, Wang J, Xu Y, et al. A multi-omics integrative analysis based on CRISPR screens re-defines the pluripotency regulatory network in ESCs. Commun Biol. 2023;6:410 pubmed publisher
Woodley K, Dillingh L, Giotopoulos G, Madrigal P, Rattigan K, Philippe C, et al. Mannose metabolism inhibition sensitizes acute myeloid leukaemia cells to therapy by driving ferroptotic cell death. Nat Commun. 2023;14:2132 pubmed publisher
Lu X, Yang W, Pei Y, Luo H, Li X, Wang Y, et al. An In Vivo CRISPR Screen Identifies That SNRPC Promotes Triple-Negative Breast Cancer Progression. Cancer Res. 2023;83:2000-2015 pubmed publisher
Althaus J, Nilius Eliliwi V, Maghnouj A, D xf6 ring S, Schroers R, Hudecek M, et al. Cytotoxicity of CD19-CAR-NK92 cells is primarily mediated via perforin/granzyme pathway. Cancer Immunol Immunother. 2023;72:2573-2583 pubmed publisher
Abuhamad A, Mohamad Zamberi N, Vanharanta S, Mohd Yusuf S, Mohtar M, Syafruddin S. Cancer Cell-Derived PDGFB Stimulates mTORC1 Activation in Renal Carcinoma. Int J Mol Sci. 2023;24: pubmed publisher
Carlson R, Leiken M, Guna A, Hacohen N, Blainey P. A genome-wide optical pooled screen reveals regulators of cellular antiviral responses. Proc Natl Acad Sci U S A. 2023;120:e2210623120 pubmed publisher
Tian X, Zhou Y, Wang S, Gao M, Xia Y, Li Y, et al. Genome-Wide CRISPR-Cas9 Screen Identifies SMCHD1 as a Restriction Factor for Herpesviruses. MBio. 2023;14:e0054923 pubmed publisher
Mathiowetz A, Roberts M, Morgens D, Olzmann J, Li Z. Protocol for performing pooled CRISPR-Cas9 loss-of-function screens. STAR Protoc. 2023;4:102201 pubmed publisher
Maurer H, Curiel García A, Holmstrom S, Laise P, Palermo C, Sastra S, et al. Ras-dependent activation of BMAL2 regulates hypoxic metabolism in pancreatic cancer. bioRxiv. 2023;: pubmed publisher
Zhang L, He W, Fu R, Xu H. Guide-specific loss of efficiency and off-target reduction with Cas9 variants. bioRxiv. 2023;: pubmed publisher
Brambati A, Sacco O, Porcella S, Heyza J, Kareh M, Schmidt J, et al. RHINO restricts MMEJ activity to mitosis. bioRxiv. 2023;: pubmed publisher
Zhao Y, Teng H, Deng Y, Sheldon M, Martínez C, Zhang J, et al. Long noncoding RNA Malat1 inhibits Tead3-Nfatc1-mediated osteoclastogenesis to suppress osteoporosis and bone metastasis. Res Sq. 2023;: pubmed publisher
Zhang H, Kelly K, Lee J, Echeverria D, Cooper D, Panwala R, et al. Self-delivering CRISPR RNAs for AAV Co-delivery and Genome Editing in vivo. bioRxiv. 2023;: pubmed publisher
Hu J, S xe1 nchez Rivera F, Wang Z, Johnson G, Ho Y, Ganesh K, et al. STING inhibits the reactivation of dormant metastasis in lung adenocarcinoma. Nature. 2023;616:806-813 pubmed publisher
Ngo A, Puschnik A. Genome-Scale Analysis of Cellular Restriction Factors That Inhibit Transgene Expression from Adeno-Associated Virus Vectors. J Virol. 2023;97:e0194822 pubmed publisher
Papa G, Albecka A, Mallery D, Vaysburd M, Renner N, James L. IP6-stabilised HIV capsids evade cGAS/STING-mediated host immune sensing. EMBO Rep. 2023;24:e56275 pubmed publisher
Matsuzawa M, Ando T, Fukase S, Kimura M, Kume Y, Ide T, et al. The protective role of conjunctival goblet cell mucin sialylation. Nat Commun. 2023;14:1417 pubmed publisher
Trevarton A, Mears E, Moody J, Lopdell T, Chometon T, Taylor J, et al. Development of SpCas9-expressing bovine mammary epithelial cell lines. In Vitro Cell Dev Biol Anim. 2023;59:163-165 pubmed publisher
W xfc nnemann F, Fotsing Tadjo T, Beaudoin M, Lalonde S, Lo K, Kleinstiver B, et al. Multimodal CRISPR perturbations of GWAS loci associated with coronary artery disease in vascular endothelial cells. PLoS Genet. 2023;19:e1010680 pubmed publisher
H xe4 cker H. In Vitro Generation of Murine Dendritic Cells from Hoxb8-Immortalized Hematopoietic Progenitors. Methods Mol Biol. 2023;2618:93-107 pubmed publisher
Kim Y, Kim N, Okafor I, Choi S, Min S, Lee J, et al. Sniper2L is a high-fidelity Cas9 variant with high activity. Nat Chem Biol. 2023;19:972-980 pubmed publisher
Wang Z, Liang Q, Qian X, Hu B, Zheng Z, Wang J, et al. An autoimmune pleiotropic SNP modulates IRF5 alternative promoter usage through ZBTB3-mediated chromatin looping. Nat Commun. 2023;14:1208 pubmed publisher
Phan Q, Solis N, Cravener M, Swidergall M, Lin J, Huang M, et al. Candida albicans stimulates the formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infection. bioRxiv. 2023;: pubmed publisher
Tian R, Huang Z, Li L, Yuan J, Zhang Q, Meng L, et al. HPV integration generates a cellular super-enhancer which functions as ecDNA to regulate genome-wide transcription. Nucleic Acids Res. 2023;51:4237-4251 pubmed publisher
Iwasaki Y, Aksu C, Reyes M, Ay B, He Q, Bastepe M. The long-range interaction between two GNAS imprinting control regions delineates pseudohypoparathyroidism type 1B pathogenesis. J Clin Invest. 2023;133: pubmed publisher
Brabson J, Leesang T, Yap Y, Wang J, Lam M, Fang B, et al. Oxidized mC modulates synthetic lethality to PARP inhibitors for the treatment of leukemia. Cell Rep. 2023;42:112027 pubmed publisher
Chang C, Lin B, Chen C, Nguyen N, Hsieh T, Su J, et al. Stellettin B Induces Cell Death in Bladder Cancer Via Activating the Autophagy/DAPK2/Apoptosis Signaling Cascade. Mar Drugs. 2023;21: pubmed publisher
Kroken A, Klein K, Mitchell P, Nieto V, Jedel E, Evans D, et al. Intracellular replication of Pseudomonas aeruginosa in epithelial cells requires suppression of the caspase-4 inflammasome. bioRxiv. 2023;: pubmed publisher
Rosenberger G, Li W, Turunen M, He J, Subramaniam P, Pampou S, et al. Network-based elucidation of colon cancer drug resistance by phosphoproteomic time-series analysis. bioRxiv. 2023;: pubmed publisher
Mohammed Ismail W, Mazzone A, Ghiraldini F, Kaur J, Bains M, Munankarmy A, et al. MacroH2A histone variants modulate enhancer activity to repress oncogenic programs and cellular reprogramming. Commun Biol. 2023;6:215 pubmed publisher
Peng X, Liu X, Hu W, Zhou Y, Ouyang L, Peng X, et al. HOXC11 drives lung adenocarcinoma progression through transcriptional regulation of SPHK1. Cell Death Dis. 2023;14:153 pubmed publisher
Rahman M, Ravichandran R, Sankpal N, Bansal S, Sureshbabu A, Fleming T, et al. Downregulation of a tumor suppressor gene LKB1 in lung transplantation as a biomarker for chronic murine lung allograft rejection. Cell Immunol. 2023;386:104690 pubmed publisher
Kuehnle N, Osborne S, Liang Z, Manzano M, Gottwein E. CRISPR screens identify novel regulators of cFLIP dependency and ligand-independent, TRAIL-R1-mediated cell death. Cell Death Differ. 2023;30:1221-1234 pubmed publisher
Li F, Wang Y, Hwang I, Jang J, Xu L, Deng Z, et al. Histone demethylase KDM2A is a selective vulnerability of cancers relying on alternative telomere maintenance. bioRxiv. 2023;: pubmed publisher
Iyer P, Zhang B, Liu T, Jin M, Hart K, Zhang J, et al. MGA deletion leads to Richter's transformation via modulation of mitochondrial OXPHOS. bioRxiv. 2023;: pubmed publisher
Zhang F, Yang S, Liao L, Zhang T, Zhang Y, Hu S, et al. Dynamic SUMOylation of MORC2 orchestrates chromatin remodelling and DNA repair in response to DNA damage and drives chemoresistance in breast cancer. Theranostics. 2023;13:973-990 pubmed publisher
Jin D, Chen X, Liu Y, Williams C, Pedersen L, Stafford D, et al. A genome-wide CRISPR-Cas9 knockout screen identifies FSP1 as the warfarin-resistant vitamin K reductase. Nat Commun. 2023;14:828 pubmed publisher
Hu M, Yang B, Jensen T, Radda J, Cheng Y, Jin S, et al. High-content image-based CRISPR screening reveals regulators of 3D genome architectures. bioRxiv. 2023;: pubmed publisher
Al Adhami H, Vallet J, Schaal C, Schumacher P, Bardet A, Dumas M, et al. Systematic identification of factors involved in the silencing of germline genes in mouse embryonic stem cells. Nucleic Acids Res. 2023;51:3130-3149 pubmed publisher
Vad Nielsen J, Staunstrup N, Kjeldsen M, Dybdal N, Flandin G, De Stradis C, et al. Genome-wide epigenetic and mRNA-expression profiling followed by CRISPR/Cas9-mediated gene-disruptions corroborate the MIR141/MIR200C-ZEB1/ZEB2-FGFR1 axis in acquired EMT-associated EGFR TKI-resistance in NSCLC cells. Transl Lung Cancer Res. 2023;12:42-65 pubmed publisher
Sapozhnikov D, Szyf M. The PROTECTOR strategy employs dCas orthologs to sterically shield off-target sites from CRISPR/Cas activity. Sci Rep. 2023;13:2280 pubmed publisher
Rehfeld F, Eitson J, Ohlson M, Chang T, Schoggins J, Mendell J. CRISPR screening reveals a dependency on ribosome recycling for efficient SARS-CoV-2 programmed ribosomal frameshifting and viral replication. Cell Rep. 2023;42:112076 pubmed publisher
Ganesan S, Alvarez N, Steiner S, Fowler K, Corona A, Roy C. Syntaxin 11 Contributes to the Interferon-Inducible Restriction of Coxiella burnetii Intracellular Infection. MBio. 2023;14:e0354522 pubmed publisher
Badenes M, Burbridge E, Oikonomidi I, Amin A, de Carvalho x, Kosack L, et al. The ADAM17 sheddase complex regulator iTAP/Frmd8 modulates inflammation and tumor growth. Life Sci Alliance. 2023;6: pubmed publisher
Honeywell M, Isidor M, Harper N, Fontana R, Gordillo P, Porto S, et al. p53 controls choice between apoptotic and non-apoptotic death following DNA damage. bioRxiv. 2023;: pubmed publisher
Virtanen L, Holm E, Halme M, West G, Lindholm F, Gullmets J, et al. Lamin A/C phosphorylation at serine 22 is a conserved heat shock response to regulate nuclear adaptation during stress. J Cell Sci. 2023;136: pubmed publisher
Zhou J, Sarkar M, Okamura K, Harris J, Gudjonsson J, Fitzgerald K. Activation of the NLRP1 inflammasome in human keratinocytes by the dsDNA mimetic poly(dA:dT). Proc Natl Acad Sci U S A. 2023;120:e2213777120 pubmed publisher
Nassar A, Abou Alaiwi S, Baca S, Adib E, Corona R, Seo J, et al. Epigenomic charting and functional annotation of risk loci in renal cell carcinoma. Nat Commun. 2023;14:346 pubmed publisher
Wistner S, Macdonald I, Stanley K, Hathaway N. Characterization of Hepatoma-Derived Growth Factor-Related Protein 2 Interactions with Heterochromatin. Cells. 2023;12: pubmed publisher
Tsujino T, Takai T, Hinohara K, Gui F, Tsutsumi T, Bai X, et al. CRISPR screens reveal genetic determinants of PARP inhibitor sensitivity and resistance in prostate cancer. Nat Commun. 2023;14:252 pubmed publisher
Islam Z, Saravanan B, Walavalkar K, Farooq U, Singh A, Radhakrishnan S, et al. Active enhancers strengthen insulation by RNA-mediated CTCF binding at chromatin domain boundaries. Genome Res. 2023;33:1-17 pubmed publisher
R xf8 mer T, Khoder Agha F, Aasted M, de Haan N, Horn S, Dylander A, et al. CRISPR-screen identifies ZIP9 and dysregulated Zn2+ homeostasis as a cause of cancer-associated changes in glycosylation. Glycobiology. 2023;: pubmed publisher
Han L, Dong L, Leung K, Zhao Z, Li Y, Gao L, et al. METTL16 drives leukemogenesis and leukemia stem cell self-renewal by reprogramming BCAA metabolism. Cell Stem Cell. 2023;30:52-68.e13 pubmed publisher
Wang R, Bhatt A, Minden Birkenmaier B, Travis O, Tiwari S, Jia H, et al. ZBTB18 restricts chromatin accessibility and prevents transcriptional adaptations that drive metastasis. Sci Adv. 2023;9:eabq3951 pubmed publisher
Wang S, Li K, Pickholz E, Dobie R, Matchett K, Henderson N, et al. An autocrine signaling circuit in hepatic stellate cells underlies advanced fibrosis in nonalcoholic steatohepatitis. Sci Transl Med. 2023;15:eadd3949 pubmed publisher
Martinez A, Kim A, Flores C, Rahman D, Yang W. Mouse embryonic stem cell-derived motor neurons are susceptible to ferroptosis. FEBS Open Bio. 2023;: pubmed publisher
Aakula A, Isomursu A, Rupp C, Erickson A, Gupta N, Kauko O, et al. PP2A methylesterase PME-1 suppresses anoikis and is associated with therapy relapse of PTEN-deficient prostate cancers. Mol Oncol. 2023;17:1007-1023 pubmed publisher
Soto Feliciano Y, S xe1 nchez Rivera F, Perner F, Barrows D, Kastenhuber E, Ho Y, et al. A Molecular Switch between Mammalian MLL Complexes Dictates Response to Menin-MLL Inhibition. Cancer Discov. 2023;13:146-169 pubmed publisher
Muscolini M, Hiscott J, Tassone E. A Genome-Wide CRISPR-Cas9 Loss-of-Function Screening to Identify Host Restriction Factors Modulating Oncolytic Virotherapy. Methods Mol Biol. 2023;2589:379-399 pubmed publisher
Thus Y, de Rooij M, Swier N, Beijersbergen R, Guikema J, Kersten M, et al. Inhibition of casein kinase 2 sensitizes mantle cell lymphoma to venetoclax through MCL-1 downregulation. Haematologica. 2023;108:797-810 pubmed publisher
Whelan J, Singaravelu R, Wang F, Pelin A, Tamming L, Pugliese G, et al. CRISPR-mediated rapid arming of poxvirus vectors enables facile generation of the novel immunotherapeutic STINGPOX. Front Immunol. 2022;13:1050250 pubmed publisher
Arang N, Lubrano S, Rigiracciolo D, Nachmanson D, Lippman S, Mali P, et al. Whole-genome CRISPR screening identifies PI3K/AKT as a downstream component of the oncogenic GNAQ-Focal Adhesion Kinase signaling circuitry. J Biol Chem. 2022;:102866 pubmed publisher
Wang E, Pineda J, Kim W, Chen S, Bourcier J, Stahl M, et al. Modulation of RNA splicing enhances response to BCL2 inhibition in leukemia. Cancer Cell. 2022;: pubmed publisher
Xu X, Chan A, Li M, Liu Q, Mattson N, Pangeni Pokharel S, et al. ACTR5 controls CDKN2A and tumor progression in an INO80-independent manner. Sci Adv. 2022;8:eadc8911 pubmed publisher
Pan Y, Han H, Hu H, Wang H, Song Y, Hao Y, et al. KMT2D deficiency drives lung squamous cell carcinoma and hypersensitivity to RTK-RAS inhibition. Cancer Cell. 2022;: pubmed publisher
Li Y, Moriyama T, Yoshimura S, Zhao X, Li Z, Yang X, et al. PAX5 epigenetically orchestrates CD58 transcription and modulates blinatumomab response in acute lymphoblastic leukemia. Sci Adv. 2022;8:eadd6403 pubmed publisher
Xu Y, Wang Z, Wei P, Gairola R, Kelsey K, Sikora A, et al. Hypermethylation of nc886 in HPV-positive oropharyngeal cancer and its clinical implications: An epigenome-wide association study. Mol Ther Nucleic Acids. 2022;30:596-605 pubmed publisher
Peng N, Kang H, Feng Y, Minikes A, Jiang X. Autophagy inhibition signals through senescence to promote tumor suppression. Autophagy. 2022;:1-17 pubmed publisher
Liu X, Sato N, Yabushita T, Li J, Jia Y, Tamura M, et al. IMPDH inhibition activates TLR-VCAM1 pathway and suppresses the development of MLL-fusion leukemia. EMBO Mol Med. 2022;:e15631 pubmed publisher
Seibert M, Kurrle N, Stolp V, N xfc rnberger H, Tzschentke S, B xf6 rner L, et al. Selection-free endogenous tagging of cell lines by bicistronic co-expression of the surface antigen NGFR. MethodsX. 2022;9:101929 pubmed publisher
Ling V, Straube J, Godfrey W, Haldar R, Janardhanan Y, Cooper L, et al. Targeting cell cycle and apoptosis to overcome chemotherapy resistance in acute myeloid leukemia. Leukemia. 2022;: pubmed publisher
Liu Y, Azizian N, Sullivan D, Li Y. mTOR inhibition attenuates chemosensitivity through the induction of chemotherapy resistant persisters. Nat Commun. 2022;13:7047 pubmed publisher
SCHMITT M, Ceteci F, Gupta J, Pesic M, B xf6 ttger T, Nicolas A, et al. Colon tumour cell death causes mTOR dependence by paracrine P2X4 stimulation. Nature. 2022;612:347-353 pubmed publisher
Chen H, Gesumaria L, Park Y, Oliver T, Singer D, Ge K, et al. BET Inhibitors Target the SCLC-N subtype of Small Cell Lung Cancer by Blocking NEUROD1 Transactivation. Mol Cancer Res. 2022;: pubmed publisher
Tian S, Xiong X, Zeng J, Wang S, Tremblay B, Chen P, et al. Identification of TFPI as a receptor reveals recombination-driven receptor switching in Clostridioides difficile toxin B variants. Nat Commun. 2022;13:6786 pubmed publisher
Han B, Liu Z, Hu X, Ling H. HNRNPU promotes the progression of triple-negative breast cancer via RNA transcription and alternative splicing mechanisms. Cell Death Dis. 2022;13:940 pubmed publisher
Hanzl A, Casement R, Imrichová H, Hughes S, Barone E, Testa A, et al. Functional E3 ligase hotspots and resistance mechanisms to small-molecule degraders. Nat Chem Biol. 2022;: pubmed publisher
Ferreira D, Idris A, McMillan N. Analysis of a hit-and-run tumor model by HPV in oropharyngeal cancers. J Med Virol. 2022;:e28260 pubmed publisher
Jin R, Niu C, Wu F, Zhou S, Han T, Zhang Z, et al. DNA damage contributes to age-associated differences in SARS-CoV-2 infection. Aging Cell. 2022;21:e13729 pubmed publisher
Merle N, Elmsh xe4 user S, Strassheimer F, Wanzel M, K xf6 nig A, Funk J, et al. Monitoring autochthonous lung tumors induced by somatic CRISPR gene editing in mice using a secreted luciferase. Mol Cancer. 2022;21:191 pubmed publisher
Tsu B, Agarwal R, Gokhale N, Kulsuptrakul J, Ryan A, Castro L, et al. Host specific sensing of coronaviruses and picornaviruses by the CARD8 inflammasome. bioRxiv. 2022;: pubmed publisher
Bonaventure B, Rebendenne A, Chaves Valad xe3 o A, Arnaud Arnould M, Gracias S, García de Gracia F, et al. The DEAD box RNA helicase DDX42 is an intrinsic inhibitor of positive-strand RNA viruses. EMBO Rep. 2022;23:e54061 pubmed publisher
Peters C, Schulze Gahmen U, Eckhardt M, Jang G, Xu J, Pulido E, et al. Structure-function analysis of enterovirus protease 2A in complex with its essential host factor SETD3. Nat Commun. 2022;13:5282 pubmed publisher
Deng S, Wang C, Wang Y, Xu Y, Li X, Johnson N, et al. Ectopic JAK-STAT activation enables the transition to a stem-like and multilineage state conferring AR-targeted therapy resistance. Nat Cancer. 2022;3:1071-1087 pubmed publisher
Liu Y, Li Q, Alikarami F, Barrett D, Mahdavi L, Li H, et al. Small-Molecule Inhibition of the Acyl-Lysine Reader ENL as a Strategy against Acute Myeloid Leukemia. Cancer Discov. 2022;12:2684-2709 pubmed publisher
McKernan C, Khatri A, Hannigan M, Child J, Chen Q, Mayro B, et al. ABL kinases regulate translation in HER2+ cells through Y-box-binding protein 1 to facilitate colonization of the brain. Cell Rep. 2022;40:111268 pubmed publisher
Stenvall C, Nystr xf6 m J, Butler Hallissey C, Jansson T, Heikkil xe4 T, Adam S, et al. Cytoplasmic keratins couple with and maintain nuclear envelope integrity in colonic epithelial cells. Mol Biol Cell. 2022;33:ar121 pubmed publisher
Tseng H, Mota Sydor A, Leventis R, Jovanovic P, Topisirovic I, Duchaine T. Distinct, opposing functions for CFIm59 and CFIm68 in mRNA alternative polyadenylation of Pten and in the PI3K/Akt signalling cascade. Nucleic Acids Res. 2022;50:9397-9412 pubmed publisher
Rodr xed guez T, Kwan S, Smith J, Dadafarin S, Wu C, Sontheimer E, et al. Multiomics characterization of mouse hepatoblastoma identifies yes-associated protein 1 target genes. Hepatology. 2022;: pubmed publisher
Biering S, Sarnik S, Wang E, Zengel J, Leist S, Sch xe4 fer A, et al. Genome-wide bidirectional CRISPR screens identify mucins as host factors modulating SARS-CoV-2 infection. Nat Genet. 2022;54:1078-1089 pubmed publisher
Xu Z, Choi J, Dai D, Luo J, Ladak R, Li Q, et al. SARS-CoV-2 impairs interferon production via NSP2-induced repression of mRNA translation. Proc Natl Acad Sci U S A. 2022;119:e2204539119 pubmed publisher
Swiderska Syn M, Mir Pedrol J, Oles A, Schleuger O, Salvador A, Greiner S, et al. Noncanonical activation of GLI signaling in SOX2+ cells drives medulloblastoma relapse. Sci Adv. 2022;8:eabj9138 pubmed publisher
Kong R, Patel A, Sato T, Jiang F, Yoo S, Bao L, et al. Transcriptional Circuitry of NKX2-1 and SOX1 Defines an Unrecognized Lineage Subtype of Small-Cell Lung Cancer. Am J Respir Crit Care Med. 2022;206:1480-1494 pubmed publisher
Yang L, Fye M, Yang B, Tang Z, Zhang Y, Haigh S, et al. Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion. Mol Metab. 2022;63:101541 pubmed publisher
Schmieder S, Tatituri R, Anderson M, Kelly K, Lencer W. Structural basis for acyl chain control over glycosphingolipid sorting and vesicular trafficking. Cell Rep. 2022;40:111063 pubmed publisher
Yang H, Liu B, Liu D, Yang Z, Zhang S, Xu P, et al. Genome-Wide CRISPR Screening Identifies DCK and CCNL1 as Genes That Contribute to Gemcitabine Resistance in Pancreatic Cancer. Cancers (Basel). 2022;14: pubmed publisher
P xe9 rez Schindler J, Vargas Fern xe1 ndez E, Karrer Cardel B, Ritz D, Schmidt A, Handschin C. Characterization of regulatory transcriptional mechanisms in hepatocyte lipotoxicity. Sci Rep. 2022;12:11477 pubmed publisher
Lei X, Ketelut Carneiro N, Shmuel Galia L, Xu W, Wilson R, Vierbuchen T, et al. Epithelial HNF4A shapes the intraepithelial lymphocyte compartment via direct regulation of immune signaling molecules. J Exp Med. 2022;219: pubmed publisher
Höfle J, Trenkner T, Kleist N, Schwane V, Vollmers S, Barcelona B, et al. Engagement of TRAIL triggers degranulation and IFNγ production in human natural killer cells. EMBO Rep. 2022;:e54133 pubmed publisher
Kolling L, Tatti R, Lowry T, Loeven A, Fadool J, Fadool D. Modulating the excitability of olfactory output neurons affects whole-body metabolism. J Neurosci. 2022;: pubmed publisher
Lu Z, Ni K, Wang Y, Zhou Y, Li Y, Yan J, et al. An in-library ligation strategy and its application in CRISPR/Cas9 screening of high-order gRNA combinations. Nucleic Acids Res. 2022;: pubmed publisher
Wei W, Li Y, Wang C, Gao S, Zhao Y, Yang Z, et al. Diterpenoid Vinigrol specifically activates ATF4/DDIT3-mediated PERK arm of unfolded protein response to drive non-apoptotic death of breast cancer cells. Pharmacol Res. 2022;182:106285 pubmed publisher
Yamaguchi K, Yoshihiro T, Ariyama H, Ito M, Nakano M, Semba Y, et al. Potential therapeutic targets discovery by transcriptome analysis of an in vitro human gastric signet ring carcinoma model. Gastric Cancer. 2022;: pubmed publisher
Iida K, Tsuchiya A, Tamura M, Yamamoto K, Kawata S, Ishihara Sugano M, et al. RUNX1 Inhibition Using Lipid Nanoparticle-Mediated Silencing RNA Delivery as an Effective Treatment for Acute Leukemias. Exp Hematol. 2022;: pubmed publisher
Li Z, Ferguson L, Deol K, Roberts M, Magtanong L, Hendricks J, et al. Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability. Nat Chem Biol. 2022;18:751-761 pubmed publisher
Pellegrino R, Castoldi M, Ticconi F, Skawran B, Budczies J, Rose F, et al. LINC00152 Drives a Competing Endogenous RNA Network in Human Hepatocellular Carcinoma. Cells. 2022;11: pubmed publisher
Jacquemin G, Wurmser A, Huyghe M, Sun W, Homayed Z, Merle C, et al. Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme. elife. 2022;11: pubmed publisher
Aubrey B, Cutler J, Bourgeois W, Donovan K, Gu S, Hatton C, et al. IKAROS and MENIN coordinate therapeutically actionable leukemogenic gene expression in MLL-r acute myeloid leukemia. Nat Cancer. 2022;3:595-613 pubmed publisher
Yang F, Sun R, Hou Z, Zhang F, Xiao Y, Yang Y, et al. HSP90 N-terminal inhibitors target oncoprotein MORC2 for autophagic degradation and suppress MORC2-driven breast cancer progression. Clin Transl Med. 2022;12:e825 pubmed publisher
Davidson R, Weaver S, Casey N, Kanojia S, Hogarth E, Schneider Aguirre R, et al. The Chd4 subunit of the NuRD complex regulates Pdx1-controlled genes involved in β-cell function. J Mol Endocrinol. 2022;69:329-341 pubmed publisher
Liu J, Knopp K, Rackaityte E, Wang C, Laurie M, Sunshine S, et al. Genome-Wide Knockout Screen Identifies Human Sialomucin CD164 as an Essential Entry Factor for Lymphocytic Choriomeningitis Virus. MBio. 2022;13:e0020522 pubmed publisher
Liu J, Wang X, Chen A, Gao X, Himes B, Zhang H, et al. ZNF117 regulates glioblastoma stem cell differentiation towards oligodendroglial lineage. Nat Commun. 2022;13:2196 pubmed publisher
de Rooij M, Thus Y, Swier N, Beijersbergen R, Pals S, Spaargaren M. A loss-of-adhesion CRISPR-Cas9 screening platform to identify cell adhesion-regulatory proteins and signaling pathways. Nat Commun. 2022;13:2136 pubmed publisher
Chen B, Hu J, Hu X, Chen H, Bao R, Zhou Y, et al. DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion. Nat Commun. 2022;13:2059 pubmed publisher
Chong Y, Tartey S, Yoshikawa Y, Imami K, Li S, Yoshinaga M, et al. Cyclin J-CDK complexes limit innate immune responses by reducing proinflammatory changes in macrophage metabolism. Sci Signal. 2022;15:eabm5011 pubmed publisher
Liu H, Wilson K, Firth A, Macri C, Schriek P, Blum A, et al. Ubiquitin-like protein 3 (UBL3) is required for MARCH ubiquitination of major histocompatibility complex class II and CD86. Nat Commun. 2022;13:1934 pubmed publisher
Xie L, Dong P, Qi Y, Hsieh T, English B, Jung S, et al. BRD2 compartmentalizes the accessible genome. Nat Genet. 2022;54:481-491 pubmed publisher
Ke P, Chang C, Hsiao Y. Baicalein Activates Parkin-Dependent Mitophagy through NDP52 and OPTN. Cells. 2022;11: pubmed publisher
Pal A, Agredo A, Lanman N, Son J, Sohal I, Bains M, et al. Loss of KMT5C Promotes EGFR Inhibitor Resistance in NSCLC via LINC01510-Mediated Upregulation of MET. Cancer Res. 2022;82:1534-1547 pubmed publisher
Apriamashvili G, Vredevoogd D, Krijgsman O, Bleijerveld O, Ligtenberg M, de Bruijn B, et al. Ubiquitin ligase STUB1 destabilizes IFNγ-receptor complex to suppress tumor IFNγ signaling. Nat Commun. 2022;13:1923 pubmed publisher
Piao X, Meng D, Zhang X, Song Q, Lv H, Jia Y. Dual-gRNA approach with limited off-target effect corrects C9ORF72 repeat expansion in vivo. Sci Rep. 2022;12:5672 pubmed publisher
Frey N, Tortola L, Egli D, Janjuha S, Rothgangl T, Marquart K, et al. Loss of Rnf31 and Vps4b sensitizes pancreatic cancer to T cell-mediated killing. Nat Commun. 2022;13:1804 pubmed publisher
Zhou H, Wang X, Steer C, Song G, Niu J. Efficient silencing of hepatitis B virus S gene through CRISPR-mediated base editing. Hepatol Commun. 2022;6:1652-1663 pubmed publisher
Yoo S, Sinha A, Yang D, Altorki N, Tandon R, Wang W, et al. Integrative network analysis of early-stage lung adenocarcinoma identifies aurora kinase inhibition as interceptor of invasion and progression. Nat Commun. 2022;13:1592 pubmed publisher
Sela Y, Li J, Maheswaran S, Norgard R, Yuan S, Hubbi M, et al. Bcl-xL Enforces a Slow-Cycling State Necessary for Survival in the Nutrient-Deprived Microenvironment of Pancreatic Cancer. Cancer Res. 2022;82:1890-1908 pubmed publisher
Kastenhuber E, Mercadante M, Nilsson Payant B, Johnson J, Jaimes J, Muecksch F, et al. Coagulation factors directly cleave SARS-CoV-2 spike and enhance viral entry. elife. 2022;11: pubmed publisher
Tian S, Liu Y, Appleton E, Wang H, Church G, Dong M. Targeted intracellular delivery of Cas13 and Cas9 nucleases using bacterial toxin-based platforms. Cell Rep. 2022;38:110476 pubmed publisher
Genolet O, Ravid Lustig L, Schulz E. Dissecting Molecular Phenotypes Through FACS-Based Pooled CRISPR Screens. Methods Mol Biol. 2022;: pubmed publisher
Shen H, Xu X, Fu Z, Xu C, Wang Y. The interactions of CAP and LYN with the insulin signaling transducer CBL play an important role in polycystic ovary syndrome. Metabolism. 2022;131:155164 pubmed publisher
Jiang C, Qian M, Gocho Y, Yang W, Du G, Shen S, et al. Genome-wide CRISPR/Cas9 screening identifies determinant of panobinostat sensitivity in acute lymphoblastic leukemia. Blood Adv. 2022;6:2496-2509 pubmed publisher
Moubarak R, de Pablos Aragoneses A, Ortiz Barahona V, Gong Y, Gowen M, Dolgalev I, et al. The histone demethylase PHF8 regulates TGFβ signaling and promotes melanoma metastasis. Sci Adv. 2022;8:eabi7127 pubmed publisher
Miao C, Tsujino T, Takai T, Gui F, Tsutsumi T, Sztupinszki Z, et al. RB1 loss overrides PARP inhibitor sensitivity driven by RNASEH2B loss in prostate cancer. Sci Adv. 2022;8:eabl9794 pubmed publisher
Sanchez Rivera F, Diaz B, Kastenhuber E, Schmidt H, Katti A, Kennedy M, et al. Base editing sensor libraries for high-throughput engineering and functional analysis of cancer-associated single nucleotide variants. Nat Biotechnol. 2022;40:862-873 pubmed publisher
Ortabozkoyun H, Huang P, Cho H, Narendra V, LeRoy G, González Buendía E, et al. CRISPR and biochemical screens identify MAZ as a cofactor in CTCF-mediated insulation at Hox clusters. Nat Genet. 2022;54:202-212 pubmed publisher
Su R, Dong L, Li Y, Gao M, He P, Liu W, et al. METTL16 exerts an m6A-independent function to facilitate translation and tumorigenesis. Nat Cell Biol. 2022;24:205-216 pubmed publisher
Miyake M, Sobajima M, Kurahashi K, Shigenaga A, Denda M, Otaka A, et al. Identification of an endoplasmic reticulum proteostasis modulator that enhances insulin production in pancreatic β cells. Cell Chem Biol. 2022;29:996-1009.e9 pubmed publisher
Falcomatà C, Bärthel S, Widholz S, Schneeweis C, Montero J, Toska A, et al. Selective multi-kinase inhibition sensitizes mesenchymal pancreatic cancer to immune checkpoint blockade by remodeling the tumor microenvironment. Nat Cancer. 2022;3:318-336 pubmed publisher
Yan Y, Zhou B, Qian C, Vasquez A, Kamra M, Chatterjee A, et al. Receptor-interacting protein kinase 2 (RIPK2) stabilizes c-Myc and is a therapeutic target in prostate cancer metastasis. Nat Commun. 2022;13:669 pubmed publisher
Morgens D, Nandakumar D, Didychuk A, Yang K, Glaunsinger B. A Two-tiered functional screen identifies herpesviral transcriptional modifiers and their essential domains. PLoS Pathog. 2022;18:e1010236 pubmed publisher
Han H, Nakaoka H, Hofmann L, Zhou J, Yu C, Zeng L, et al. The Hippo pathway kinases LATS1 and LATS2 attenuate cellular responses to heavy metals through phosphorylating MTF1. Nat Cell Biol. 2022;24:74-87 pubmed publisher
Luke C, Markovina S, Good M, Wight I, Thomas B, Linneman J, et al. Lysoptosis is an evolutionarily conserved cell death pathway moderated by intracellular serpins. Commun Biol. 2022;5:47 pubmed publisher
Kattan R, Han H, Seo G, Yang B, Lin Y, Dotson M, et al. Interactome Analysis of Human Phospholipase D and Phosphatidic Acid-Associated Protein Network. Mol Cell Proteomics. 2022;21:100195 pubmed publisher
Raveendra Panickar D, Finlay D, Layng F, Lambert L, Celeridad M, Zhao M, et al. Discovery of novel furanylbenzamide inhibitors that target oncogenic tyrosine phosphatase SHP2 in leukemia cells. J Biol Chem. 2022;298:101477 pubmed publisher
Fennell K, Vassiliadis D, Lam E, Martelotto L, Balic J, Hollizeck S, et al. Non-genetic determinants of malignant clonal fitness at single-cell resolution. Nature. 2022;601:125-131 pubmed publisher
Singh H, Seruggia D, Madha S, Saxena M, Nagaraja A, Wu Z, et al. Transcription factor-mediated intestinal metaplasia and the role of a shadow enhancer. Genes Dev. 2021;: pubmed publisher
Cona B, Hayashi T, Yamada A, Shimizu N, Yokota N, Nakato R, et al. The splicing factor DHX38/PRP16 is required for ovarian clear cell carcinoma tumorigenesis, as revealed by a CRISPR-Cas9 screen. FEBS Open Bio. 2021;: pubmed publisher
Tang C, Clark O, Ferrarone J, Campos C, Lalani A, Chodera J, et al. GCN2 kinase activation by ATP-competitive kinase inhibitors. Nat Chem Biol. 2021;: pubmed publisher
Clark L, Clark S, Lin C, Liu J, Coscia A, Nabel K, et al. VLDLR and ApoER2 are receptors for multiple alphaviruses. Nature. 2021;: pubmed publisher
Wu B, Rice L, Shrimpton J, Lawless D, Walker K, Carter C, et al. Biallelic mutations in calcium release activated channel regulator 2A (CRACR2A) cause a primary immunodeficiency disorder. elife. 2021;10: pubmed publisher
Yan Y, Zhou B, Lee Y, You S, Freeman M, Yang W. BoxCar and shotgun proteomic analyses reveal molecular networks regulated by UBR5 in prostate cancer. Proteomics. 2021;:e2100172 pubmed publisher
Olivieri A, Lee R, Fratini F, Keutcha C, Chaand M, Mangano V, et al. Structural organization of erythrocyte membrane microdomains and their relation with malaria susceptibility. Commun Biol. 2021;4:1375 pubmed publisher
Liu Y, Wu Z, Zhou J, Ramadurai D, Mortenson K, Aguilera Jimenez E, et al. A predominant enhancer co-amplified with the SOX2 oncogene is necessary and sufficient for its expression in squamous cancer. Nat Commun. 2021;12:7139 pubmed publisher
Yan F, Li J, Milosević J, Petroni R, Liu S, Shi Z, et al. KAT6A and ENL form an epigenetic transcriptional control module to drive critical leukemogenic gene expression programs. Cancer Discov. 2021;: pubmed publisher
Lin J, Guo D, Liu H, Zhou W, Wang C, Muller I, et al. The SETDB1-TRIM28 Complex Suppresses Antitumor Immunity. Cancer Immunol Res. 2021;9:1413-1424 pubmed publisher
Feng Y, Li C, Stewart J, Barbulescu P, Desivo N, Alvarez Quilón A, et al. FAM72A antagonizes UNG2 to promote mutagenic repair during antibody maturation. Nature. 2021;600:324-328 pubmed publisher
Barroso Gomila O, Trulsson F, Muratore V, Canosa I, Merino Cacho L, Cortazar A, et al. Identification of proximal SUMO-dependent interactors using SUMO-ID. Nat Commun. 2021;12:6671 pubmed publisher
Lu Y, Shen H, Huang W, He S, Chen J, Zhang D, et al. Genome-scale CRISPR-Cas9 knockout screening in hepatocellular carcinoma with lenvatinib resistance. Cell Death Discov. 2021;7:359 pubmed publisher
Missios P, da Rocha E, Pearson D, Philipp J, Aleman M, Pirouz M, et al. LIN28B alters ribosomal dynamics to promote metastasis in MYCN-driven malignancy. J Clin Invest. 2021;131: pubmed publisher
Spliid C, Toledo A, Sanderson P, Mao Y, Gatto F, Gustavsson T, et al. The specificity of the malarial VAR2CSA protein for chondroitin sulfate depends on 4-O-sulfation and ligand accessibility. J Biol Chem. 2021;297:101391 pubmed publisher
Gaidt M, Morrow A, Fairgrieve M, Karr J, Yosef N, Vance R. Self-guarding of MORC3 enables virulence factor-triggered immunity. Nature. 2021;600:138-142 pubmed publisher
Pan M, Wright W, Chapple R, Zubair A, Sandhu M, Batchelder J, et al. The chemotherapeutic CX-5461 primarily targets TOP2B and exhibits selective activity in high-risk neuroblastoma. Nat Commun. 2021;12:6468 pubmed publisher
Farooq U, Notani D. Optimized protocol to create deletion in adherent cell lines using CRISPR/Cas9 system. STAR Protoc. 2021;2:100857 pubmed publisher
Trofimenko E, Grasso G, Heulot M, Chevalier N, Deriu M, Dubuis G, et al. Genetic, cellular, and structural characterization of the membrane potential-dependent cell-penetrating peptide translocation pore. elife. 2021;10: pubmed publisher
Liew H, Meister S, Mein S, Tessonnier T, Köpp B, Held T, et al. Combined DNA Damage Repair Interference and Ion Beam Therapy: Development, Benchmark, and Clinical Implications of a Mechanistic Biological Model. Int J Radiat Oncol Biol Phys. 2021;: pubmed publisher
Schnoeder T, Schwarzer A, Jayavelu A, Hsu C, Kirkpatrick J, Dohner K, et al. PLCG1 is required for AML1-ETO leukemia stem cell self-renewal. Blood. 2021;: pubmed publisher
Kitakaze K, Tsuboi K, Tsuda M, Takenouchi Y, Ishimaru H, Okamoto Y. Development of a selective fluorescence-based enzyme assay for glycerophosphodiesterase family members GDE4 and GDE7. J Lipid Res. 2021;62:100141 pubmed publisher
Zinshteyn B, Sinha N, Enam S, Koleske B, Green R. Translational repression of NMD targets by GIGYF2 and EIF4E2. PLoS Genet. 2021;17:e1009813 pubmed publisher
Oliveira T, Zhang M, Joo E, Abdel Azim H, Chen C, Yang L, et al. Glycoproteome remodeling in MLL-rearranged B-cell precursor acute lymphoblastic leukemia. Theranostics. 2021;11:9519-9537 pubmed publisher
Okamoto Y, Kitakaze K, Takenouchi Y, Yamamoto S, Ishimaru H, Tsuboi K. Sphingosine 1-phosphate receptor type 2 positively regulates interleukin (IL)-4/IL-13-induced STAT6 phosphorylation. Cell Signal. 2021;88:110156 pubmed publisher
Sasaki K, Yamauchi T, Semba Y, Nogami J, Imanaga H, Terasaki T, et al. Genome-wide CRISPR-Cas9 screen identifies rationally designed combination therapies for CRLF2-rearranged Ph-like ALL. Blood. 2021;: pubmed publisher
Chu J, Xing C, Du Y, Duan T, Liu S, Zhang P, et al. Pharmacological inhibition of fatty acid synthesis blocks SARS-CoV-2 replication. Nat Metab. 2021;3:1466-1475 pubmed publisher
Romine K, Nechiporuk T, Bottomly D, Jeng S, McWeeney S, Kaempf A, et al. Monocytic differentiation and AHR signaling as Primary Nodes of BET Inhibitor Response in Acute Myeloid Leukemia. Blood Cancer Discov. 2021;2:518-531 pubmed publisher
Kitakaze K, Oyadomari M, Zhang J, Hamada Y, Takenouchi Y, Tsuboi K, et al. ATF4-mediated transcriptional regulation protects against β-cell loss during endoplasmic reticulum stress in a mouse model. Mol Metab. 2021;54:101338 pubmed publisher
Xue Y, Morris J, Yang K, Fu Z, Zhu X, Johnson F, et al. SMARCA4/2 loss inhibits chemotherapy-induced apoptosis by restricting IP3R3-mediated Ca2+ flux to mitochondria. Nat Commun. 2021;12:5404 pubmed publisher
Bagayoko S, Leon Icaza S, Pinilla M, Hessel A, Santoni K, Pericat D, et al. Host phospholipid peroxidation fuels ExoU-dependent cell necrosis and supports Pseudomonas aeruginosa-driven pathology. PLoS Pathog. 2021;17:e1009927 pubmed publisher
Quambusch L, Depta L, Landel I, Lubeck M, Kirschner T, Nabert J, et al. Cellular model system to dissect the isoform-selectivity of Akt inhibitors. Nat Commun. 2021;12:5297 pubmed publisher
Jang H, Jo D, Cho C, Shin J, Seo J, Yu G, et al. Application of prime editing to the correction of mutations and phenotypes in adult mice with liver and eye diseases. Nat Biomed Eng. 2021;: pubmed publisher
Peer E, Aichberger S, Vilotic F, Gruber W, Parigger T, Grund Gröschke S, et al. Casein Kinase 1D Encodes a Novel Drug Target in Hedgehog-GLI-Driven Cancers and Tumor-Initiating Cells Resistant to SMO Inhibition. Cancers (Basel). 2021;13: pubmed publisher
Wu B, Woo J, Vila P, Jew M, Leung J, Sun Z, et al. NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca2+ entry in T cells. Cell Rep. 2021;36:109603 pubmed publisher
Akasu M, Shimada S, Kabashima A, Akiyama Y, Shimokawa M, Akahoshi K, et al. Intrinsic activation of β-catenin signaling by CRISPR/Cas9-mediated exon skipping contributes to immune evasion in hepatocellular carcinoma. Sci Rep. 2021;11:16732 pubmed publisher
Deisl C, Hilgemann D, Syeda R, Fine M. TMEM16F and dynamins control expansive plasma membrane reservoirs. Nat Commun. 2021;12:4990 pubmed publisher
Parma B, Ramesh V, Gollavilli P, Siddiqui A, Pinna L, Schwab A, et al. Metabolic impairment of non-small cell lung cancers by mitochondrial HSPD1 targeting. J Exp Clin Cancer Res. 2021;40:248 pubmed publisher
Feng W, Cao Z, Lim P, Zhao H, Luo H, Mao N, et al. Rapid interrogation of cancer cell of origin through CRISPR editing. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
Ciampricotti M, Karakousi T, Richards A, Quintanal Villalonga Á, Karatza A, Caeser R, et al. Rlf-Mycl gene fusion drives tumorigenesis and metastasis in a mouse model of small cell lung cancer. Cancer Discov. 2021;: pubmed publisher
Pierce S, Granja J, Corces M, Brady J, Tsai M, Pierce A, et al. LKB1 inactivation modulates chromatin accessibility to drive metastatic progression. Nat Cell Biol. 2021;23:915-924 pubmed publisher
Malinova D, Wasim L, Newman R, Martínez Riaño A, Engels N, Tolar P. Endophilin A2 regulates B-cell endocytosis and is required for germinal center and humoral responses. EMBO Rep. 2021;:e51328 pubmed publisher
Zhang Q, Agius S, Flanigan S, Uckelmann M, Levina V, Owen B, et al. PALI1 facilitates DNA and nucleosome binding by PRC2 and triggers an allosteric activation of catalysis. Nat Commun. 2021;12:4592 pubmed publisher
Wu S, Xiao Y, Wei J, Xu X, Jin X, Hu X, et al. MYC suppresses STING-dependent innate immunity by transcriptionally upregulating DNMT1 in triple-negative breast cancer. J Immunother Cancer. 2021;9: pubmed publisher
Gao M, Wang J, Rousseaux S, Tan M, Pan L, Peng L, et al. Metabolically controlled histone H4K5 acylation/acetylation ratio drives BRD4 genomic distribution. Cell Rep. 2021;36:109460 pubmed publisher
Vancura A, Lanzós A, Bosch Guiteras N, Esteban M, Gutierrez A, Haefliger S, et al. Cancer LncRNA Census 2 (CLC2): an enhanced resource reveals clinical features of cancer lncRNAs. NAR Cancer. 2021;3:zcab013 pubmed publisher
Sahu B, Pihlajamaa P, Zhang K, Palin K, Ahonen S, Cervera A, et al. Human cell transformation by combined lineage conversion and oncogene expression. Oncogene. 2021;: pubmed publisher
Kaseke C, Park R, Singh N, Koundakjian D, Bashirova A, Garcia Beltran W, et al. HLA class-I-peptide stability mediates CD8+ T cell immunodominance hierarchies and facilitates HLA-associated immune control of HIV. Cell Rep. 2021;36:109378 pubmed publisher
Li Y, Chen B, Zhao J, Li Q, Chen S, Guo T, et al. HNRNPL Circularizes ARHGAP35 to Produce an Oncogenic Protein. Adv Sci (Weinh). 2021;8:2001701 pubmed publisher
Li Z, Bruno V, Kim K. CNS-infecting pathogens Escherichia coli and Cryptococcus neoformans exploit the host Pdlim2 for intracellular traversal and exocytosis in the blood-brain barrier. Infect Immun. 2021;:IAI0012821 pubmed publisher
Meier Abt F, Lu J, Cannizzaro E, Pohly M, Kummer S, Pfammatter S, et al. The Protein Landscape of Chronic Lymphocytic Leukemia (CLL). Blood. 2021;: pubmed publisher
Conway E, Rossi F, Fernandez Perez D, Ponzo E, Ferrari K, Zanotti M, et al. BAP1 enhances Polycomb repression by counteracting widespread H2AK119ub1 deposition and chromatin condensation. Mol Cell. 2021;: pubmed publisher
Zhou J, Gelot C, Pantelidou C, Li A, Yücel H, Davis R, et al. A first-in-class Polymerase Theta Inhibitor selectively targets Homologous-Recombination-Deficient Tumors. Nat Cancer. 2021;2:598-610 pubmed publisher
Lu S, De Neef E, Thomas J, SABIO E, Rousseau B, Gigoux M, et al. Pharmacologic modulation of RNA splicing enhances anti-tumor immunity. Cell. 2021;184:4032-4047.e31 pubmed publisher
Zhou X, Li J, Chen Z, Ni W, Li X, Yang R, et al. Dependency of human and murine LKB1-inactivated lung cancer on aberrant CRTC-CREB activation. elife. 2021;10: pubmed publisher
Wan C, Mahara S, Sun C, Doan A, Chua H, Xu D, et al. Genome-scale CRISPR-Cas9 screen of Wnt/β-catenin signaling identifies therapeutic targets for colorectal cancer. Sci Adv. 2021;7: pubmed publisher
Xu Y, Peng X, Zheng Y, Jin C, Lu X, Han D, et al. Inactivation of Latent HIV-1 Proviral DNA Using Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Treatment and the Assessment of Off-Target Effects. Front Microbiol. 2021;12:629153 pubmed publisher
Garcia Garcia S, Rodrigo Faus M, Fonseca N, Manzano S, Gyorffy B, Ocana A, et al. HGK promotes metastatic dissemination in prostate cancer. Sci Rep. 2021;11:12287 pubmed publisher
Takahashi K, Niki T, Ogawa E, Fumika K, Nishioka Y, Sawa M, et al. A cell-free assay implicates a role of sphingomyelin and cholesterol in STING phosphorylation. Sci Rep. 2021;11:11996 pubmed publisher
Poole E, Carlan da Silva M, Huang C, Perera M, Jackson S, Groves I, et al. A BMPR2/YY1 Signaling Axis Is Required for Human Cytomegalovirus Latency in Undifferentiated Myeloid Cells. MBio. 2021;12:e0022721 pubmed publisher
Datlinger P, Rendeiro A, Boenke T, Senekowitsch M, Krausgruber T, Barreca D, et al. Ultra-high-throughput single-cell RNA sequencing and perturbation screening with combinatorial fluidic indexing. Nat Methods. 2021;18:635-642 pubmed publisher
Ramaker R, Hardigan A, Gordon E, Wright C, Myers R, Cooper S. Pooled CRISPR screening in pancreatic cancer cells implicates co-repressor complexes as a cause of multiple drug resistance via regulation of epithelial-to-mesenchymal transition. BMC Cancer. 2021;21:632 pubmed publisher
Trimarco J, Heaton B, Chaparian R, Burke K, Binder R, Gray G, et al. TMEM41B is a host factor required for the replication of diverse coronaviruses including SARS-CoV-2. PLoS Pathog. 2021;17:e1009599 pubmed publisher
Fan Y, Köberlin M, Ratnayeke N, Liu C, Deshpande M, Gerhardt J, et al. LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components. J Cell Biol. 2021;220: pubmed publisher
Tamura T, Kodama T, Sato K, Murai K, Yoshioka T, Shigekawa M, et al. Dysregulation of PI3K and Hippo signaling pathways synergistically induces chronic pancreatitis via CTGF upregulation. J Clin Invest. 2021;131: pubmed publisher
Muller I, Moroni A, Shlyueva D, Sahadevan S, Schoof E, Radzisheuskaya A, et al. MPP8 is essential for sustaining self-renewal of ground-state pluripotent stem cells. Nat Commun. 2021;12:3034 pubmed publisher
Cheng D, Oni T, Thalappillil J, Park Y, Ting H, Alagesan B, et al. Oncogenic KRAS engages an RSK1/NF1 pathway to inhibit wild-type RAS signaling in pancreatic cancer. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
Zhou X, Xiong H, Lu Y, Geng M, Lu Y, Zhang L, et al. PKD2 deficiency suppresses amino acid biosynthesis in ADPKD by impairing the PERK-TBL2-eIF2ɑ-ATF4 pathway. Biochem Biophys Res Commun. 2021;561:73-79 pubmed publisher
Zhao Y, Jiang S, Zhang J, Guan X, Sun B, Sun L. A virulent Bacillus cereus strain from deep-sea cold seep induces pyroptosis in a manner that involves NLRP3 inflammasome, JNK pathway, and lysosomal rupture. Virulence. 2021;12:1362-1376 pubmed publisher
Mimura K, Sakamaki J, Morishita H, Kawazu M, Mano H, Mizushima N. Genome-wide CRISPR screening reveals nucleotide synthesis negatively regulates autophagy. J Biol Chem. 2021;296:100780 pubmed publisher
Ganga A, Kennedy M, Oguchi M, Gray S, Oliver K, Knight T, et al. Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway. Curr Biol. 2021;31:2895-2905.e7 pubmed publisher
Meng D, Yang Q, Melick C, Park B, Hsieh T, Curukovic A, et al. ArfGAP1 inhibits mTORC1 lysosomal localization and activation. EMBO J. 2021;40:e106412 pubmed publisher
Zhemkov V, Ditlev J, Lee W, Wilson M, Liou J, Rosen M, et al. The role of sigma 1 receptor in organization of endoplasmic reticulum signaling microdomains. elife. 2021;10: pubmed publisher
Liscovitch Brauer N, Montalbano A, Deng J, Méndez Mancilla A, Wessels H, Moss N, et al. Profiling the genetic determinants of chromatin accessibility with scalable single-cell CRISPR screens. Nat Biotechnol. 2021;: pubmed publisher
Hayman T, Baro M, MacNeil T, Phoomak C, Aung T, Cui W, et al. STING enhances cell death through regulation of reactive oxygen species and DNA damage. Nat Commun. 2021;12:2327 pubmed publisher
Chen M, Zhao Y, Yang X, Zhao Y, Liu Q, Liu Y, et al. NSDHL promotes triple-negative breast cancer metastasis through the TGFβ signaling pathway and cholesterol biosynthesis. Breast Cancer Res Treat. 2021;187:349-362 pubmed publisher
Genolet O, Monaco A, Dunkel I, Boettcher M, Schulz E. Identification of X-chromosomal genes that drive sex differences in embryonic stem cells through a hierarchical CRISPR screening approach. Genome Biol. 2021;22:110 pubmed publisher
Vinjamur D, Yao Q, Cole M, McGuckin C, Ren C, Zeng J, et al. ZNF410 represses fetal globin by singular control of CHD4. Nat Genet. 2021;53:719-728 pubmed publisher
Shinoda K, Zong D, Callen E, Wu W, Dumitrache L, Belinky F, et al. The dystonia gene THAP1 controls DNA double-strand break repair choice. Mol Cell. 2021;81:2611-2624.e10 pubmed publisher
Simoneschi D, Róna G, Zhou N, Jeong Y, Jiang S, Milletti G, et al. CRL4AMBRA1 is a master regulator of D-type cyclins. Nature. 2021;592:789-793 pubmed publisher
Mo J, Liu F, Sun X, Huang H, Tan K, Zhao X, et al. Inhibition of the FACT Complex Targets Aberrant Hedgehog Signaling and Overcomes Resistance to Smoothened Antagonists. Cancer Res. 2021;81:3105-3120 pubmed publisher
Su D, Wang W, Hou Y, Wang L, Yi X, Cao C, et al. Bimodal regulation of the PRC2 complex by USP7 underlies tumorigenesis. Nucleic Acids Res. 2021;49:4421-4440 pubmed publisher
Inoue D, Polaski J, Taylor J, Castel P, Chen S, Kobayashi S, et al. Minor intron retention drives clonal hematopoietic disorders and diverse cancer predisposition. Nat Genet. 2021;53:707-718 pubmed publisher
Kwon A, Mohri K, Takizawa S, Arakawa T, Takahashi M, Kaczkowski B, et al. Development of p53 knockout U87MG cell line for unbiased drug delivery testing system using CRISPR-Cas9 and transcriptomic analysis. J Biotechnol. 2021;332:72-82 pubmed publisher
Nakamura T, Ogawa M, Kojima K, Takayanagi S, Ishihara S, Hattori K, et al. The mitochondrial Ca2+ uptake regulator, MICU1, is involved in cold stress-induced ferroptosis. EMBO Rep. 2021;22:e51532 pubmed publisher
Kastenhuber E, Jaimes J, Johnson J, Mercadante M, Muecksch F, Weisblum Y, et al. Coagulation factors directly cleave SARS-CoV-2 spike and enhance viral entry. bioRxiv. 2021;: pubmed publisher
Shriwas O, Arya R, Mohanty S, Mohapatra P, Kumar S, Rath R, et al. RRBP1 rewires cisplatin resistance in oral squamous cell carcinoma by regulating Hippo pathway. Br J Cancer. 2021;124:2004-2016 pubmed publisher
Fujino T, Goyama S, Sugiura Y, Inoue D, Asada S, Yamasaki S, et al. Mutant ASXL1 induces age-related expansion of phenotypic hematopoietic stem cells through activation of Akt/mTOR pathway. Nat Commun. 2021;12:1826 pubmed publisher
Warwick T, Schulz M, Günther S, Gilsbach R, Neme A, Carlberg C, et al. A hierarchical regulatory network analysis of the vitamin D induced transcriptome reveals novel regulators and complete VDR dependency in monocytes. Sci Rep. 2021;11:6518 pubmed publisher
Kulsuptrakul J, Wang R, Meyers N, Ott M, Puschnik A. A genome-wide CRISPR screen identifies UFMylation and TRAMP-like complexes as host factors required for hepatitis A virus infection. Cell Rep. 2021;34:108859 pubmed publisher
Samart P, Luanpitpong S, Rojanasakul Y, Issaragrisil S. O-GlcNAcylation homeostasis controlled by calcium influx channels regulates multiple myeloma dissemination. J Exp Clin Cancer Res. 2021;40:100 pubmed publisher
Maruoka M, Zhang P, Mori H, Imanishi E, Packwood D, Harada H, et al. Caspase cleavage releases a nuclear protein fragment that stimulates phospholipid scrambling at the plasma membrane. Mol Cell. 2021;81:1397-1410.e9 pubmed publisher
Radzisheuskaya A, Shliaha P, Grinev V, Shlyueva D, Damhofer H, Koche R, et al. Complex-dependent histone acetyltransferase activity of KAT8 determines its role in transcription and cellular homeostasis. Mol Cell. 2021;81:1749-1765.e8 pubmed publisher
Liu N, Xu S, Yao Q, Zhu Q, Kai Y, Hsu J, et al. Transcription factor competition at the γ-globin promoters controls hemoglobin switching. Nat Genet. 2021;53:511-520 pubmed publisher
Quijada Álamo M, Pérez Carretero C, Hernández Sánchez M, Rodríguez Vicente A, Herrero A, Hernández Sánchez J, et al. Dissecting the role of TP53 alterations in del(11q) chronic lymphocytic leukemia. Clin Transl Med. 2021;11:e304 pubmed publisher
Han D, Owiredu J, Healy B, Li M, Labaf M, Steinfeld J, et al. Susceptibility-Associated Genetic Variation in NEDD9 Contributes to Prostate Cancer Initiation and Progression. Cancer Res. 2021;81:3766-3776 pubmed publisher
Heyes E, Schmidt L, Manhart G, Eder T, Proietti L, Grebien F. Identification of gene targets of mutant C/EBPα reveals a critical role for MSI2 in CEBPA-mutated AML. Leukemia. 2021;: pubmed publisher
Chan W, Gottschalk R, Yao Y, Pomerantz J, Germain R. Efficient Immune Cell Genome Engineering with Enhanced CRISPR Editing Tools. Immunohorizons. 2021;5:117-132 pubmed publisher
Zhu Y, Feng F, Hu G, Wang Y, Yu Y, Zhu Y, et al. A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry. Nat Commun. 2021;12:961 pubmed publisher
Bosch Guiteras N, Uroda T, Guillén Ramírez H, Riedo R, Gazdhar A, Esposito R, et al. Enhancing CRISPR deletion via pharmacological delay of DNA-PKcs. Genome Res. 2021;31:461-471 pubmed publisher
Shin H, See J, Kweon J, Kim H, Sung G, Park S, et al. Small-molecule inhibitors of histone deacetylase improve CRISPR-based adenine base editing. Nucleic Acids Res. 2021;: pubmed publisher
Song N, Chen L, Ren X, Waterfield N, Yang J, Yang G. N-Glycans and sulfated glycosaminoglycans contribute to the action of diverse Tc toxins on mammalian cells. PLoS Pathog. 2021;17:e1009244 pubmed publisher
Phoomak C, Cui W, Hayman T, Yu S, Zhao P, Wells L, et al. The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress. Sci Adv. 2021;7: pubmed publisher
Sheppard H, Dall Agnese A, Park W, Shamim M, Dubrulle J, Johnson H, et al. Targeted brachyury degradation disrupts a highly specific autoregulatory program controlling chordoma cell identity. Cell Rep Med. 2021;2:100188 pubmed publisher
Patel A, Yoo S, Kong R, Sato T, Sinha A, Karam S, et al. Prototypical oncogene family Myc defines unappreciated distinct lineage states of small cell lung cancer. Sci Adv. 2021;7: pubmed publisher
McLellan L, McAllaster M, Kim A, Tothova L, Olson P, Pinkner J, et al. A host receptor enables type 1 pilus-mediated pathogenesis of Escherichia coli pyelonephritis. PLoS Pathog. 2021;17:e1009314 pubmed publisher
Li C, Peng Z, Wang Y, Lam G, Nissen N, Tang J, et al. Epithelial Cell Transforming 2 (ECT2) is regulated by Yes-associated protein 1 (YAP1) and mediates pancreatic cancer progression and metastasis. Am J Physiol Gastrointest Liver Physiol. 2021;: pubmed publisher
Ni W, Chen Z, Zhou X, Yang R, Yu M, Lu J, et al. Targeting Notch and EGFR signaling in human mucoepidermoid carcinoma. Signal Transduct Target Ther. 2021;6:27 pubmed publisher
Mittler E, Alkutkar T, Jangra R, Chandran K. Direct Intracellular Visualization of Ebola Virus-Receptor Interaction by In Situ Proximity Ligation. MBio. 2021;12: pubmed publisher
Oh S, Lee H, Ahn M, Jeon M, Yoon J, Jung Y, et al. Multiplex gene targeting in the mouse embryo using a Cas9-Cpf1 hybrid guide RNA. Biochem Biophys Res Commun. 2021;539:48-55 pubmed publisher
Shirasaki R, Matthews G, Gandolfi S, de Matos Simoes R, Buckley D, Raja Vora J, et al. Functional Genomics Identify Distinct and Overlapping Genes Mediating Resistance to Different Classes of Heterobifunctional Degraders of Oncoproteins. Cell Rep. 2021;34:108532 pubmed publisher
Schneider W, Luna J, Hoffmann H, Sanchez Rivera F, Leal A, Ashbrook A, et al. Genome-Scale Identification of SARS-CoV-2 and Pan-coronavirus Host Factor Networks. Cell. 2021;184:120-132.e14 pubmed publisher
Damnernsawad A, Bottomly D, Kurtz S, Eide C, McWeeney S, Tyner J, et al. Genome-wide CRISPR screen identifies regulators of MAPK and MTOR pathways mediating sorafenib resistance in acute myeloid leukemia. Haematologica. 2020;Online ahead of print: pubmed publisher
Bahri H, Buratto J, Rojo M, Dompierre J, Salin B, Blancard C, et al. TMEM70 forms oligomeric scaffolds within mitochondrial cristae promoting in situ assembly of mammalian ATP synthase proton channel. Biochim Biophys Acta Mol Cell Res. 2021;1868:118942 pubmed publisher
Hoffmann H, Sanchez Rivera F, Schneider W, Luna J, Soto Feliciano Y, Ashbrook A, et al. Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors. Cell Host Microbe. 2021;29:267-280.e5 pubmed publisher
Jia X, Burugula B, Chen V, Lemons R, Jayakody S, Maksutova M, et al. Massively parallel functional testing of MSH2 missense variants conferring Lynch syndrome risk. Am J Hum Genet. 2021;108:163-175 pubmed publisher
Myojin Y, Hikita H, Sugiyama M, Sasaki Y, Fukumoto K, Sakane S, et al. Hepatic Stellate Cells in Hepatocellular Carcinoma Promote Tumor Growth Via Growth Differentiation Factor 15 Production. Gastroenterology. 2021;160:1741-1754.e16 pubmed publisher
Hulton C, Costa E, Shah N, Quintanal Villalonga Á, Heller G, de Stanchina E, et al. Direct genome editing of patient-derived xenografts using CRISPR-Cas9 enables rapid in vivo functional genomics. Nat Cancer. 2020;1:359-369 pubmed publisher
Hoffmann H, Schneider W, Rozen Gagnon K, Miles L, Schuster F, Razooky B, et al. TMEM41B Is a Pan-flavivirus Host Factor. Cell. 2021;184:133-148.e20 pubmed publisher
Wang R, Simoneau C, Kulsuptrakul J, Bouhaddou M, Travisano K, Hayashi J, et al. Genetic Screens Identify Host Factors for SARS-CoV-2 and Common Cold Coronaviruses. Cell. 2021;184:106-119.e14 pubmed publisher
Xiang Y, Li X, Zhan Z, Feng J, Cai H, Li Y, et al. A Novel Nonsense GLI3 Variant Is Associated With Polydactyly and Syndactyly in a Family by Blocking the Sonic Hedgehog Signaling Pathway. Front Genet. 2020;11:542004 pubmed publisher
Markiewicz Potoczny M, Lobanova A, Loeb A, Kirak O, Olbrich T, Ruiz S, et al. TRF2-mediated telomere protection is dispensable in pluripotent stem cells. Nature. 2021;589:110-115 pubmed publisher
Zhan T, Faehling V, Rauscher B, Betge J, Ebert M, Boutros M. Multi-omics integration identifies a selective vulnerability of colorectal cancer subtypes to YM155. Int J Cancer. 2020;: pubmed publisher
Petitjean O, Girardi E, Ngondo R, Lupashin V, Pfeffer S. Genome-Wide CRISPR-Cas9 Screen Reveals the Importance of the Heparan Sulfate Pathway and the Conserved Oligomeric Golgi Complex for Synthetic Double-Stranded RNA Uptake and Sindbis Virus Infection. mSphere. 2020;5: pubmed publisher
Carey K, Paulus G, Wang L, Balce D, Luo J, Bergman P, et al. TFEB Transcriptional Responses Reveal Negative Feedback by BHLHE40 and BHLHE41. Cell Rep. 2020;33:108371 pubmed publisher
Gao Z, van der Velden Y, Fan M, van der Linden C, Vink M, Herrera Carrillo E, et al. Engineered mini H1 promoters with dedicated RNA Polymerase II or III activity. J Biol Chem. 2020;: pubmed publisher
Mabe N, Garcia N, Wolery S, Newcomb R, Meingasner R, Vilona B, et al. G9a Promotes Breast Cancer Recurrence through Repression of a Pro-inflammatory Program. Cell Rep. 2020;33:108341 pubmed publisher
Wei J, Alfajaro M, DeWeirdt P, Hanna R, Lu Culligan W, Cai W, et al. Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection. Cell. 2021;184:76-91.e13 pubmed publisher
Biasci D, Smoragiewicz M, Connell C, Wang Z, Gao Y, Thaventhiran J, et al. CXCR4 inhibition in human pancreatic and colorectal cancers induces an integrated immune response. Proc Natl Acad Sci U S A. 2020;117:28960-28970 pubmed publisher
Liu Y, Guo B, Aguilera Jimenez E, Chu V, Zhou J, Wu Z, et al. Chromatin Looping Shapes KLF5-Dependent Transcriptional Programs in Human Epithelial Cancers. Cancer Res. 2020;80:5464-5477 pubmed publisher
Korvatska O, Kiianitsa K, Ratushny A, Matsushita M, Beeman N, Chien W, et al. Triggering Receptor Expressed on Myeloid Cell 2 R47H Exacerbates Immune Response in Alzheimer's Disease Brain. Front Immunol. 2020;11:559342 pubmed publisher
Liu J, Zuo Z, Zou M, Finkel T, Liu S. Identification of the transcription factor Miz1 as an essential regulator of diphthamide biosynthesis using a CRISPR-mediated genome-wide screen. PLoS Genet. 2020;16:e1009068 pubmed publisher
Lian B, Pei Y, Jiang Y, Xue M, Li D, Li X, et al. Truncated HDAC9 identified by integrated genome-wide screen as the key modulator for paclitaxel resistance in triple-negative breast cancer. Theranostics. 2020;10:11092-11109 pubmed publisher
Schieber M, Marinaccio C, Bolanos L, Haffey W, Greis K, Starczynowski D, et al. FBXO11 is a candidate tumor suppressor in the leukemic transformation of myelodysplastic syndrome. Blood Cancer J. 2020;10:98 pubmed publisher
Chen Y, Jiang T, Zhang H, Gou X, Han C, Wang J, et al. LRRC31 inhibits DNA repair and sensitizes breast cancer brain metastasis to radiation therapy. Nat Cell Biol. 2020;22:1276-1285 pubmed publisher
Clausen T, Sandoval D, Spliid C, Pihl J, Perrett H, Painter C, et al. SARS-CoV-2 Infection Depends on Cellular Heparan Sulfate and ACE2. Cell. 2020;: pubmed publisher
Zhang Q, Cao C, Gong W, Bao K, Wang Q, Wang Y, et al. A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis. Theranostics. 2020;10:10769-10790 pubmed publisher
Lu S, Zhang Y, Yin H. Chimeric DNA-RNA Guide RNA Designs. Methods Mol Biol. 2021;2162:79-85 pubmed publisher
Chen J, Harding S, Natesan R, Tian L, Benci J, Li W, et al. Cell Cycle Checkpoints Cooperate to Suppress DNA- and RNA-Associated Molecular Pattern Recognition and Anti-Tumor Immune Responses. Cell Rep. 2020;32:108080 pubmed publisher
Lv L, Chen P, Cao L, Li Y, Zeng Z, Cui Y, et al. Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation. elife. 2020;9: pubmed publisher
Bortnik V, Wu M, Julcher B, Salinas A, Nikolic I, Simpson K, et al. Loss of HPV type 16 E7 restores cGAS-STING responses in human papilloma virus-positive oropharyngeal squamous cell carcinomas cells. J Microbiol Immunol Infect. 2020;: pubmed publisher
Oldrini B, Vaquero Siguero N, Mu Q, Kroon P, Zhang Y, Galán Ganga M, et al. MGMT genomic rearrangements contribute to chemotherapy resistance in gliomas. Nat Commun. 2020;11:3883 pubmed publisher
Mayor Ruiz C, Bauer S, Brand M, Kozicka Z, Siklos M, Imrichová H, et al. Rational discovery of molecular glue degraders via scalable chemical profiling. Nat Chem Biol. 2020;: pubmed publisher
Liu G, Ruan Y, Zhang J, Wang X, Wu W, He P, et al. ABHD11 Is Critical for Embryonic Stem Cell Expansion, Differentiation and Lipid Metabolic Homeostasis. Front Cell Dev Biol. 2020;8:570 pubmed publisher
Pavani G, Laurent M, Fabiano A, Cantelli E, Sakkal A, Corre G, et al. Ex vivo editing of human hematopoietic stem cells for erythroid expression of therapeutic proteins. Nat Commun. 2020;11:3778 pubmed publisher
Noorani I, de la Rosa J, Choi Y, Strong A, Ponstingl H, Vijayabaskar M, et al. PiggyBac mutagenesis and exome sequencing identify genetic driver landscapes and potential therapeutic targets of EGFR-mutant gliomas. Genome Biol. 2020;21:181 pubmed publisher
Sur Erdem I, Muslu K, Pınarbası N, Altunbek M, Seker Polat F, Cingöz A, et al. TRAIL-conjugated silver nanoparticles sensitize glioblastoma cells to TRAIL by regulating CHK1 in the DNA repair pathway. Neurol Res. 2020;42:1061-1069 pubmed publisher
Clausen T, Sandoval D, Spliid C, Pihl J, Painter C, Thacker B, et al. SARS-CoV-2 Infection Depends on Cellular Heparan Sulfate and ACE2. bioRxiv. 2020;: pubmed publisher
Fox D, Garcia N, McKinney B, Lupo R, Noteware L, Newcomb R, et al. NRF2 activation promotes the recurrence of dormant tumour cells through regulation of redox and nucleotide metabolism. Nat Metab. 2020;2:318-334 pubmed publisher
Luanpitpong S, Rodboon N, Samart P, Vinayanuwattikun C, Klamkhlai S, Chanvorachote P, et al. A novel TRPM7/O-GlcNAc axis mediates tumour cell motility and metastasis by stabilising c-Myc and caveolin-1 in lung carcinoma. Br J Cancer. 2020;: pubmed publisher
Pan Y, Tian R, Lee C, Bao G, Gibson G. Fine-mapping within eQTL credible intervals by expression CROP-seq. Biol Methods Protoc. 2020;5:bpaa008 pubmed publisher
Seo G, Han H, Vargas R, Yang B, Li X, Wang W. MAP4K Interactome Reveals STRN4 as a Key STRIPAK Complex Component in Hippo Pathway Regulation. Cell Rep. 2020;32:107860 pubmed publisher
Inde Z, Forcina G, Denton K, Dixon S. Kinetic Heterogeneity of Cancer Cell Fractional Killing. Cell Rep. 2020;32:107845 pubmed publisher
Song M, Kim H, Lee S, Kim Y, Seo S, Park J, et al. Sequence-specific prediction of the efficiencies of adenine and cytosine base editors. Nat Biotechnol. 2020;: pubmed publisher
Thomsen E, Rovsing A, Anderson M, Due H, Huang J, Luo Y, et al. Identification of BLNK and BTK as mediators of rituximab-induced programmed cell death by CRISPR screens in GCB-subtype diffuse large B-cell lymphoma. Mol Oncol. 2020;: pubmed publisher
Mei Y, Han X, Liu Y, Yang J, Sumagin R, Ji P. Diaphanous-related formin mDia2 regulates beta2 integrins to control hematopoietic stem and progenitor cell engraftment. Nat Commun. 2020;11:3172 pubmed publisher
Bozal Basterra L, Gonzalez Santamarta M, Muratore V, Bermejo Arteagabeitia A, Da Fonseca C, Barroso Gomila O, et al. LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is a contributing factor in Townes-Brocks Syndrome. elife. 2020;9: pubmed publisher
Liu J, Zuo Z, Sastalla I, Liu C, Jang J, Sekine Y, et al. Sequential CRISPR-Based Screens Identify LITAF and CDIP1 as the Bacillus cereus Hemolysin BL Toxin Host Receptors. Cell Host Microbe. 2020;: pubmed publisher
Wandel M, Kim B, Park E, Boyle K, Nayak K, Lagrange B, et al. Guanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms. Nat Immunol. 2020;21:880-891 pubmed publisher
Su R, Dong L, Li Y, Gao M, Han L, Wunderlich M, et al. Targeting FTO Suppresses Cancer Stem Cell Maintenance and Immune Evasion. Cancer Cell. 2020;38:79-96.e11 pubmed publisher
Alix E, Godlee C, Cerny O, Blundell S, Tocci R, Matthews S, et al. The Tumour Suppressor TMEM127 Is a Nedd4-Family E3 Ligase Adaptor Required by Salmonella SteD to Ubiquitinate and Degrade MHC Class II Molecules. Cell Host Microbe. 2020;28:54-68.e7 pubmed publisher
Wessels H, Méndez Mancilla A, Guo X, Legut M, Daniloski Z, Sanjana N. Massively parallel Cas13 screens reveal principles for guide RNA design. Nat Biotechnol. 2020;38:722-727 pubmed publisher
Gregory J, Hoelzli E, Abdelaal R, Braine C, Cuevas M, Halpern M, et al. Cell Type-Specific In Vitro Gene Expression Profiling of Stem Cell-Derived Neural Models. Cells. 2020;9: pubmed publisher
Song Q, Ni K, Liu M, Li Y, Wang L, Wang Y, et al. Direct-seq: programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen. Genome Biol. 2020;21:136 pubmed publisher
Hutter K, Lohmüller M, Jukic A, Eichin F, Avci S, Labi V, et al. SAFB2 Enables the Processing of Suboptimal Stem-Loop Structures in Clustered Primary miRNA Transcripts. Mol Cell. 2020;78:876-889.e6 pubmed publisher
Zhang Y, Le T, Grabau R, Mohseni Z, Kim H, Natale D, et al. TMEM16F phospholipid scramblase mediates trophoblast fusion and placental development. Sci Adv. 2020;6:eaba0310 pubmed publisher
Pinch B, Doctor Z, Nabet B, Browne C, Seo H, Mohardt M, et al. Identification of a potent and selective covalent Pin1 inhibitor. Nat Chem Biol. 2020;: pubmed publisher
Zhu N, Liu W, Prakash A, Zhang C, Kim K. Targeting E. coli invasion of the blood-brain barrier for investigating the pathogenesis and therapeutic development of E. coli meningitis. Cell Microbiol. 2020;:e13231 pubmed publisher
Nichols C, Gibson W, Brown M, Kosmicki J, Busanovich J, Wei H, et al. Loss of heterozygosity of essential genes represents a widespread class of potential cancer vulnerabilities. Nat Commun. 2020;11:2517 pubmed publisher
Holthaus D, Vasou A, Bamford C, Andrejeva J, Paulus C, Randall R, et al. Direct Antiviral Activity of IFN-Stimulated Genes Is Responsible for Resistance to Paramyxoviruses in ISG15-Deficient Cells. J Immunol. 2020;205:261-271 pubmed publisher
Jaiswal P, Aljebali L, Gaumond M, Oh C, Yasuda H, Moffatt P. Biochemical characteristics of the chondrocyte-enriched SNORC protein and its transcriptional regulation by SOX9. Sci Rep. 2020;10:7790 pubmed publisher
Bare D, Cherny V, DeCoursey T, Abukhdeir A, Morgan D. Expression and function of voltage gated proton channels (Hv1) in MDA-MB-231 cells. PLoS ONE. 2020;15:e0227522 pubmed publisher
Melick C, Meng D, Jewell J. A-kinase anchoring protein 8L interacts with mTORC1 and promotes cell growth. J Biol Chem. 2020;295:8096-8105 pubmed publisher
Chung K, Singh J, Lawres L, Dorans K, Garcia C, Burkhardt D, et al. Endocrine-Exocrine Signaling Drives Obesity-Associated Pancreatic Ductal Adenocarcinoma. Cell. 2020;181:832-847.e18 pubmed publisher
Tian S, Liu Y, Wu H, Liu H, Zeng J, Choi M, et al. Genome-Wide CRISPR Screen Identifies Semaphorin 6A and 6B as Receptors for Paeniclostridium sordellii Toxin TcsL. Cell Host Microbe. 2020;27:782-792.e7 pubmed publisher
Zhou X, Li R, Jing R, Zuo B, Zheng Q. Genome-wide CRISPR knockout screens identify ADAMTSL3 and PTEN genes as suppressors of HCC proliferation and metastasis, respectively. J Cancer Res Clin Oncol. 2020;146:1509-1521 pubmed publisher
Mueller W, Jakob P, Sun H, Clauder Münster S, Ghidelli Disse S, Ordonez D, et al. Loss of N-Glycanase 1 Alters Transcriptional and Translational Regulation in K562 Cell Lines. G3 (Bethesda). 2020;10:1585-1597 pubmed publisher
Ochi Y, Kon A, Sakata T, Nakagawa M, Nakazawa N, Kakuta M, et al. Combined Cohesin-RUNX1 Deficiency Synergistically Perturbs Chromatin Looping and Causes Myelodysplastic Syndromes. Cancer Discov. 2020;10:836-853 pubmed publisher
Jun S, Lim H, Chun H, Lee J, Bang D. Single-cell analysis of a mutant library generated using CRISPR-guided deaminase in human melanoma cells. Commun Biol. 2020;3:154 pubmed publisher
Zhang Z, Zhou C, Li X, Barnes S, Deng S, Hoover E, et al. Loss of CHD1 Promotes Heterogeneous Mechanisms of Resistance to AR-Targeted Therapy via Chromatin Dysregulation. Cancer Cell. 2020;37:584-598.e11 pubmed publisher
Mancikova V, Peschelova H, Kozlova V, Ledererova A, Ladungova A, Verner J, et al. Performance of anti-CD19 chimeric antigen receptor T cells in genetically defined classes of chronic lymphocytic leukemia. J Immunother Cancer. 2020;8: pubmed publisher
Kozlova V, Ledererova A, Ladungova A, Peschelova H, Janovská P, Ślusarczyk A, et al. CD20 is dispensable for B-cell receptor signaling but is required for proper actin polymerization, adhesion and migration of malignant B cells. PLoS ONE. 2020;15:e0229170 pubmed publisher
Rathore A, Iketani S, Wang P, Jia M, Sahi V, Ho D. CRISPR-based gene knockout screens reveal deubiquitinases involved in HIV-1 latency in two Jurkat cell models. Sci Rep. 2020;10:5350 pubmed publisher
Ferrari de Andrade L, Kumar S, Luoma A, Ito Y, Alves da Silva P, Pan D, et al. Inhibition of MICA and MICB Shedding Elicits NK-Cell-Mediated Immunity against Tumors Resistant to Cytotoxic T Cells. Cancer Immunol Res. 2020;8:769-780 pubmed publisher
Binda C, Klaver B, Berkhout B, Das A. CRISPR-Cas9 Dual-gRNA Attack Causes Mutation, Excision and Inversion of the HIV-1 Proviral DNA. Viruses. 2020;12: pubmed publisher
Yuan S, Natesan R, Sanchez Rivera F, Li J, Bhanu N, Yamazoe T, et al. Global Regulation of the Histone Mark H3K36me2 Underlies Epithelial Plasticity and Metastatic Progression. Cancer Discov. 2020;10:854-871 pubmed publisher
Haga K, Ettayebi K, Tenge V, Karandikar U, Lewis M, Lin S, et al. Genetic Manipulation of Human Intestinal Enteroids Demonstrates the Necessity of a Functional Fucosyltransferase 2 Gene for Secretor-Dependent Human Norovirus Infection. MBio. 2020;11: pubmed publisher
Shinohara T, Kanatsu Shinohara M. Transgenesis and Genome Editing of Mouse Spermatogonial Stem Cells by Lentivirus Pseudotyped with Sendai Virus F Protein. Stem Cell Reports. 2020;14:447-461 pubmed publisher
Raffeiner P, Hart J, García Caballero D, Bar Peled L, Weinberg M, Vogt P. An MXD1-derived repressor peptide identifies noncoding mediators of MYC-driven cell proliferation. Proc Natl Acad Sci U S A. 2020;117:6571-6579 pubmed publisher
Liu H, Liu Y, Yang F, Zhang L, Zhang F, Hu X, et al. Acetylation of MORC2 by NAT10 regulates cell-cycle checkpoint control and resistance to DNA-damaging chemotherapy and radiotherapy in breast cancer. Nucleic Acids Res. 2020;48:3638-3656 pubmed publisher
de Jong O, Murphy D, Mäger I, Willms E, Garcia Guerra A, Gitz Francois J, et al. A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA. Nat Commun. 2020;11:1113 pubmed publisher
Ma G, He L, Liu S, Xie J, Huang Z, Jing J, et al. Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling. Nat Commun. 2020;11:1039 pubmed publisher
Chen Y, Han H, Seo G, Vargas R, Yang B, Chuc K, et al. Systematic analysis of the Hippo pathway organization and oncogenic alteration in evolution. Sci Rep. 2020;10:3173 pubmed publisher
Mao N, Gao D, Hu W, Gadal S, Hieronymus H, Wang S, et al. Oncogenic ERG Represses PI3K Signaling through Downregulation of IRS2. Cancer Res. 2020;80:1428-1437 pubmed publisher
Oladimeji P, Bakke J, Wright W, Chen T. KANSL2 and MBNL3 are regulators of pancreatic ductal adenocarcinoma invasion. Sci Rep. 2020;10:1485 pubmed publisher
Hu Q, Klages Mundt N, Wang R, Lynn E, Kuma Saha L, Zhang H, et al. The ARK Assay Is a Sensitive and Versatile Method for the Global Detection of DNA-Protein Crosslinks. Cell Rep. 2020;30:1235-1245.e4 pubmed publisher
Panda S, Wigerblad G, Jiang L, Jiménez Andrade Y, Iyer V, Shen Y, et al. IL-4 controls activated neutrophil FcγR2b expression and migration into inflamed joints. Proc Natl Acad Sci U S A. 2020;117:3103-3113 pubmed publisher
Hiranniramol K, Chen Y, Liu W, Wang X. Generalizable sgRNA design for improved CRISPR/Cas9 editing efficiency. Bioinformatics. 2020;36:2684-2689 pubmed publisher
Wilk A, Hayat F, Cunningham R, Li J, Garavaglia S, Zamani L, et al. Extracellular NAD+ enhances PARP-dependent DNA repair capacity independently of CD73 activity. Sci Rep. 2020;10:651 pubmed publisher
Chen C, Li B, Millman S, Chen C, Li X, Morris J, et al. Vitamin B6 Addiction in Acute Myeloid Leukemia. Cancer Cell. 2020;37:71-84.e7 pubmed publisher
Katsuyama E, Suárez Fueyo A, Bradley S, Mizui M, Marin A, Mulki L, et al. The CD38/NAD/SIRTUIN1/EZH2 Axis Mitigates Cytotoxic CD8 T Cell Function and Identifies Patients with SLE Prone to Infections. Cell Rep. 2020;30:112-123.e4 pubmed publisher
Thomas J, Polaski J, Feng Q, De Neef E, Hoppe E, McSharry M, et al. RNA isoform screens uncover the essentiality and tumor-suppressor activity of ultraconserved poison exons. Nat Genet. 2020;52:84-94 pubmed publisher
Zheng A, Chevalier N, Calderoni M, Dubuis G, Dormond O, Ziros P, et al. CRISPR/Cas9 genome-wide screening identifies KEAP1 as a sorafenib, lenvatinib, and regorafenib sensitivity gene in hepatocellular carcinoma. Oncotarget. 2019;10:7058-7070 pubmed publisher
Peng Y, Yang T, Tang X, Chen F, Wang S. Construction of an Inducible CRISPR/Cas9 System for CXCR4 Gene and Demonstration of its Effects on MKN-45 Cells. Cell Biochem Biophys. 2019;: pubmed publisher
Ng S, Rideout W, Akama Garren E, Bhutkar A, Mercer K, Schenkel J, et al. CRISPR-mediated modeling and functional validation of candidate tumor suppressor genes in small cell lung cancer. Proc Natl Acad Sci U S A. 2019;: pubmed publisher
Nanki K, Fujii M, Shimokawa M, Matano M, Nishikori S, Date S, et al. Somatic inflammatory gene mutations in human ulcerative colitis epithelium. Nature. 2019;: pubmed publisher
Kitakaze K, Taniuchi S, Kawano E, Hamada Y, Miyake M, Oyadomari M, et al. Cell-based HTS identifies a chemical chaperone for preventing ER protein aggregation and proteotoxicity. elife. 2019;8: pubmed publisher
Xu S, Zhan M, Jiang C, He M, Yang L, Shen H, et al. Genome-wide CRISPR screen identifies ELP5 as a determinant of gemcitabine sensitivity in gallbladder cancer. Nat Commun. 2019;10:5492 pubmed publisher
Dudek A, Zabaleta N, Zinn E, Pillay S, Zengel J, Porter C, et al. GPR108 Is a Highly Conserved AAV Entry Factor. Mol Ther. 2019;: pubmed publisher
Vargas R, Duong V, Han H, Ta A, Chen Y, Zhao S, et al. Elucidation of WW domain ligand binding specificities in the Hippo pathway reveals STXBP4 as YAP inhibitor. EMBO J. 2020;39:e102406 pubmed publisher
Yu H, Rimbert A, Palmer A, Toyohara T, Xia Y, Xia F, et al. GPR146 Deficiency Protects against Hypercholesterolemia and Atherosclerosis. Cell. 2019;179:1276-1288.e14 pubmed publisher
Pouliot G, Degar J, Hinze L, Kochupurakkal B, Vo C, Burns M, et al. Fanconi-BRCA pathway mutations in childhood T-cell acute lymphoblastic leukemia. PLoS ONE. 2019;14:e0221288 pubmed publisher
Xu D, Liang S, Yang H, Bruggmann R, Berezowska S, Yang Z, et al. CRISPR screening identifies WEE1 as a combination target for standard chemotherapy in malignant pleural mesothelioma. Mol Cancer Ther. 2019;: pubmed publisher
Chen T, Zhang B, Ziegenhals T, Prusty A, Fröhler S, Grimm C, et al. A missense mutation in SNRPE linked to non-syndromal microcephaly interferes with U snRNP assembly and pre-mRNA splicing. PLoS Genet. 2019;15:e1008460 pubmed publisher
Smits A, Ziebell F, Joberty G, Zinn N, Mueller W, Clauder Münster S, et al. Biological plasticity rescues target activity in CRISPR knock outs. Nat Methods. 2019;16:1087-1093 pubmed publisher
Hayashi Y, Goyama S, Liu X, Tamura M, Asada S, Tanaka Y, et al. Antitumor immunity augments the therapeutic effects of p53 activation on acute myeloid leukemia. Nat Commun. 2019;10:4869 pubmed publisher
Bell C, Raffeiner P, Hart J, Vogt P. PIK3CA Cooperates with KRAS to Promote MYC Activity and Tumorigenesis via the Bromodomain Protein BRD9. Cancers (Basel). 2019;11: pubmed publisher
Shohat S, Shifman S. Genes essential for embryonic stem cells are associated with neurodevelopmental disorders. Genome Res. 2019;29:1910-1918 pubmed publisher
Bersuker K, Hendricks J, Li Z, Magtanong L, Ford B, Tang P, et al. The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis. Nature. 2019;575:688-692 pubmed publisher
Anastas J, Zee B, Kalin J, Kim M, Guo R, Alexandrescu S, et al. Re-programing Chromatin with a Bifunctional LSD1/HDAC Inhibitor Induces Therapeutic Differentiation in DIPG. Cancer Cell. 2019;36:528-544.e10 pubmed publisher
Feldman D, Singh A, Schmid Burgk J, Carlson R, Mezger A, Garrity A, et al. Optical Pooled Screens in Human Cells. Cell. 2019;179:787-799.e17 pubmed publisher
Hoshino A, Wang W, Wada S, McDermott Roe C, Evans C, Gosis B, et al. The ADP/ATP translocase drives mitophagy independent of nucleotide exchange. Nature. 2019;575:375-379 pubmed publisher
Wei L, Lee D, Law C, Zhang M, Shen J, Chin D, et al. Genome-wide CRISPR/Cas9 library screening identified PHGDH as a critical driver for Sorafenib resistance in HCC. Nat Commun. 2019;10:4681 pubmed publisher
Radzisheuskaya A, Shliaha P, Grinev V, Lorenzini E, Kovalchuk S, Shlyueva D, et al. PRMT5 methylome profiling uncovers a direct link to splicing regulation in acute myeloid leukemia. Nat Struct Mol Biol. 2019;26:999-1012 pubmed publisher
Inoue D, Chew G, Liu B, Michel B, Pangallo J, D Avino A, et al. Spliceosomal disruption of the non-canonical BAF complex in cancer. Nature. 2019;574:432-436 pubmed publisher
Cheng W, Wang S, Zhang Z, Morgens D, Hayes L, Lee S, et al. CRISPR-Cas9 Screens Identify the RNA Helicase DDX3X as a Repressor of C9ORF72 (GGGGCC)n Repeat-Associated Non-AUG Translation. Neuron. 2019;104:885-898.e8 pubmed publisher
Zhou L, Mudianto T, Ma X, Riley R, Uppaluri R. Targeting EZH2 Enhances Antigen Presentation, Antitumor Immunity, and Circumvents Anti-PD-1 Resistance in Head and Neck Cancer. Clin Cancer Res. 2019;: pubmed publisher
Sato T, Yoo S, Kong R, Sinha A, Chandramani Shivalingappa P, Patel A, et al. Epigenomic Profiling Discovers Trans-lineage SOX2 Partnerships Driving Tumor Heterogeneity in Lung Squamous Cell Carcinoma. Cancer Res. 2019;79:6084-6100 pubmed publisher
Guieze R, Liu V, Rosebrock D, Jourdain A, Hernández Sánchez M, Martinez Zurita A, et al. Mitochondrial Reprogramming Underlies Resistance to BCL-2 Inhibition in Lymphoid Malignancies. Cancer Cell. 2019;36:369-384.e13 pubmed publisher
Jeng E, Bhadkamkar V, Ibe N, Gause H, Jiang L, Chan J, et al. Systematic Identification of Host Cell Regulators of Legionella pneumophila Pathogenesis Using a Genome-wide CRISPR Screen. Cell Host Microbe. 2019;26:551-563.e6 pubmed publisher
Morris J, Yashinskie J, Koche R, Chandwani R, Tian S, Chen C, et al. α-Ketoglutarate links p53 to cell fate during tumour suppression. Nature. 2019;573:595-599 pubmed publisher
Babosova O, Kapralova K, Raskova Kafkova L, Korinek V, Divoky V, Prchal J, et al. Iron chelation and 2-oxoglutarate-dependent dioxygenase inhibition suppress mantle cell lymphoma's cyclin D1. J Cell Mol Med. 2019;23:7785-7795 pubmed publisher
Kweon J, Jang A, Shin H, See J, Lee W, Lee J, et al. A CRISPR-based base-editing screen for the functional assessment of BRCA1 variants. Oncogene. 2020;39:30-35 pubmed publisher
Fraser J, Simpson J, Fontana R, Kishi Itakura C, Ktistakis N, Gammoh N. Targeting of early endosomes by autophagy facilitates EGFR recycling and signalling. EMBO Rep. 2019;20:e47734 pubmed publisher
Mayor Ruiz C, Jaeger M, Bauer S, Brand M, Sin C, Hanzl A, et al. Plasticity of the Cullin-RING Ligase Repertoire Shapes Sensitivity to Ligand-Induced Protein Degradation. Mol Cell. 2019;75:849-858.e8 pubmed publisher
Gobbi G, Donati B, do Valle I, Reggiani F, Torricelli F, Remondini D, et al. The Hippo pathway modulates resistance to BET proteins inhibitors in lung cancer cells. Oncogene. 2019;38:6801-6817 pubmed publisher
El Khattabi L, Zhao H, Kalchschmidt J, Young N, Jung S, van Blerkom P, et al. A Pliable Mediator Acts as a Functional Rather Than an Architectural Bridge between Promoters and Enhancers. Cell. 2019;178:1145-1158.e20 pubmed publisher
Jiang C, Trudeau S, Cheong T, Guo R, Teng M, Wang L, et al. CRISPR/Cas9 Screens Reveal Multiple Layers of B cell CD40 Regulation. Cell Rep. 2019;28:1307-1322.e8 pubmed publisher
Cai C, Wang X, Zhao Y, Yi C, Jin Z, Zhang A, et al. Construction of a mavs-inactivated MDCK cell line for facilitating the propagation of canine distemper virus (CDV). Mol Immunol. 2019;114:133-138 pubmed publisher
Hollstein P, Eichner L, Brun S, Kamireddy A, Svensson R, Vera L, et al. The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive Effects of LKB1 in NSCLC. Cancer Discov. 2019;9:1606-1627 pubmed publisher
D Souza Z, Blackburn J, Kudlyk T, Pokrovskaya I, Lupashin V. Defects in COG-Mediated Golgi Trafficking Alter Endo-Lysosomal System in Human Cells. Front Cell Dev Biol. 2019;7:118 pubmed publisher
Liu J, Srinivasan S, Li C, Ho I, Rose J, Shaheen M, et al. Pooled library screening with multiplexed Cpf1 library. Nat Commun. 2019;10:3144 pubmed publisher
Tang J, Gautam P, Gupta A, He L, Timonen S, Akimov Y, et al. Network pharmacology modeling identifies synergistic Aurora B and ZAK interaction in triple-negative breast cancer. NPJ Syst Biol Appl. 2019;5:20 pubmed publisher
Vredevoogd D, Kuilman T, Ligtenberg M, Boshuizen J, Stecker K, de Bruijn B, et al. Augmenting Immunotherapy Impact by Lowering Tumor TNF Cytotoxicity Threshold. Cell. 2019;178:585-599.e15 pubmed publisher
Mao N, Gao D, Hu W, Hieronymus H, Wang S, Lee Y, et al. Aberrant Expression of ERG Promotes Resistance to Combined PI3K and AR Pathway Inhibition through Maintenance of AR Target Genes. Mol Cancer Ther. 2019;18:1577-1586 pubmed publisher
Woolston A, Khan K, Spain G, Barber L, Griffiths B, Gonzalez Exposito R, et al. Genomic and Transcriptomic Determinants of Therapy Resistance and Immune Landscape Evolution during Anti-EGFR Treatment in Colorectal Cancer. Cancer Cell. 2019;36:35-50.e9 pubmed publisher
Sher F, HOSSAIN M, Seruggia D, Schoonenberg V, Yao Q, Cifani P, et al. Rational targeting of a NuRD subcomplex guided by comprehensive in situ mutagenesis. Nat Genet. 2019;51:1149-1159 pubmed publisher
Adams E, Karthaus W, Hoover E, Liu D, Gruet A, Zhang Z, et al. FOXA1 mutations alter pioneering activity, differentiation and prostate cancer phenotypes. Nature. 2019;571:408-412 pubmed publisher
Gothe H, Bouwman B, Gusmao E, Piccinno R, Petrosino G, Sayols S, et al. Spatial Chromosome Folding and Active Transcription Drive DNA Fragility and Formation of Oncogenic MLL Translocations. Mol Cell. 2019;: pubmed publisher
Chen W, McKenna A, Schreiber J, Haeussler M, Yin Y, Agarwal V, et al. Massively parallel profiling and predictive modeling of the outcomes of CRISPR/Cas9-mediated double-strand break repair. Nucleic Acids Res. 2019;47:7989-8003 pubmed publisher
MacDougall M, Clarke R, Merrill B. Intracellular Ca2+ Homeostasis and Nuclear Export Mediate Exit from Naive Pluripotency. Cell Stem Cell. 2019;: pubmed publisher
Zhao X, Zhang G, Liu S, Chen X, Peng R, Dai L, et al. Human Neonatal Fc Receptor Is the Cellular Uncoating Receptor for Enterovirus B. Cell. 2019;: pubmed publisher
Seruggia D, Oti M, Tripathi P, Canver M, LeBlanc L, Di Giammartino D, et al. TAF5L and TAF6L Maintain Self-Renewal of Embryonic Stem Cells via the MYC Regulatory Network. Mol Cell. 2019;74:1148-1163.e7 pubmed publisher
Sastre Perona A, Hoang Phou S, Leitner M, Okuniewska M, Meehan S, Schober M. De Novo PITX1 Expression Controls Bi-Stable Transcriptional Circuits to Govern Self-Renewal and Differentiation in Squamous Cell Carcinoma. Cell Stem Cell. 2019;24:390-404.e8 pubmed publisher
García Carpizo V, Ruiz Llorente S, Sarmentero J, González Corpas A, Barrero M. CREBBP/EP300 Bromodomain Inhibition Affects the Proliferation of AR-Positive Breast Cancer Cell Lines. Mol Cancer Res. 2019;17:720-730 pubmed publisher
Ji L, Zhao G, Zhang P, Huo W, Dong P, Watari H, et al. Knockout of MTF1 Inhibits the Epithelial to Mesenchymal Transition in Ovarian Cancer Cells. J Cancer. 2018;9:4578-4585 pubmed publisher
Hatzi K, Geng H, Doane A, Meydan C, LaRiviere R, Cárdenas M, et al. Histone demethylase LSD1 is required for germinal center formation and BCL6-driven lymphomagenesis. Nat Immunol. 2019;20:86-96 pubmed publisher
Gao Z, Herrera Carrillo E, Berkhout B. A Single H1 Promoter Can Drive Both Guide RNA and Endonuclease Expression in the CRISPR-Cas9 System. Mol Ther Nucleic Acids. 2018;14:32-40 pubmed publisher
Tian S, Muneeruddin K, Choi M, Tao L, Bhuiyan R, Ohmi Y, et al. Genome-wide CRISPR screens for Shiga toxins and ricin reveal Golgi proteins critical for glycosylation. PLoS Biol. 2018;16:e2006951 pubmed publisher
Yao M, Fang W, Smart C, Hu Q, Huang S, Alvarez N, et al. CCR2 Chemokine Receptors Enhance Growth and Cell-Cycle Progression of Breast Cancer Cells through SRC and PKC Activation. Mol Cancer Res. 2019;17:604-617 pubmed publisher
Riad A, Zeng C, Weng C, Winters H, Xu K, Makvandi M, et al. Sigma-2 Receptor/TMEM97 and PGRMC-1 Increase the Rate of Internalization of LDL by LDL Receptor through the Formation of a Ternary Complex. Sci Rep. 2018;8:16845 pubmed publisher
Lattanzi A, Meneghini V, Pavani G, Amor F, Ramadier S, Félix T, et al. Optimization of CRISPR/Cas9 Delivery to Human Hematopoietic Stem and Progenitor Cells for Therapeutic Genomic Rearrangements. Mol Ther. 2019;27:137-150 pubmed publisher
Yan X, Zhang X, Wang L, Zhang R, Pu X, Wu S, et al. Inhibition of Thioredoxin/Thioredoxin Reductase Induces Synthetic Lethality in Lung Cancers with Compromised Glutathione Homeostasis. Cancer Res. 2019;79:125-132 pubmed publisher
Riestra A, Valderrama J, Patras K, Booth S, Quek X, Tsai C, et al. Trichomonas vaginalis Induces NLRP3 Inflammasome Activation and Pyroptotic Cell Death in Human Macrophages. J Innate Immun. 2019;11:86-98 pubmed publisher
Biffi G, Oni T, Spielman B, Hao Y, Elyada E, Park Y, et al. IL1-Induced JAK/STAT Signaling Is Antagonized by TGFβ to Shape CAF Heterogeneity in Pancreatic Ductal Adenocarcinoma. Cancer Discov. 2019;9:282-301 pubmed publisher
Mosa M, Nicolle O, Maschalidi S, Sepulveda F, Bidaud Meynard A, Menche C, et al. Dynamic Formation of Microvillus Inclusions During Enterocyte Differentiation in Munc18-2-Deficient Intestinal Organoids. Cell Mol Gastroenterol Hepatol. 2018;6:477-493.e1 pubmed publisher
Kim J, Piao H, Kim B, Yao F, Han Z, Wang Y, et al. Long noncoding RNA MALAT1 suppresses breast cancer metastasis. Nat Genet. 2018;50:1705-1715 pubmed publisher
Sarvi S, Crispin R, Lu Y, Zeng L, Hurley T, Houston D, et al. ALDH1 Bio-activates Nifuroxazide to Eradicate ALDHHigh Melanoma-Initiating Cells. Cell Chem Biol. 2018;25:1456-1469.e6 pubmed publisher
Han H, Qi R, Zhou J, Ta A, Yang B, Nakaoka H, et al. Regulation of the Hippo Pathway by Phosphatidic Acid-Mediated Lipid-Protein Interaction. Mol Cell. 2018;72:328-340.e8 pubmed publisher
Zhong F, Robinson K, Teo D, Tan K, Lim C, Harapas C, et al. Human DPP9 represses NLRP1 inflammasome and protects against autoinflammatory diseases via both peptidase activity and FIIND domain binding. J Biol Chem. 2018;293:18864-18878 pubmed publisher
Cheruiyot A, Li S, Nickless A, Roth R, Fitzpatrick J, You Z. Compound C inhibits nonsense-mediated RNA decay independently of AMPK. PLoS ONE. 2018;13:e0204978 pubmed publisher
Gilbertson S, Federspiel J, Hartenian E, Cristea I, Glaunsinger B. Changes in mRNA abundance drive shuttling of RNA binding proteins, linking cytoplasmic RNA degradation to transcription. elife. 2018;7: pubmed publisher
Kühnle S, Martinez Noel G, Leclere F, Hayes S, Harper J, Howley P. Angelman syndrome-associated point mutations in the Zn2+-binding N-terminal (AZUL) domain of UBE3A ubiquitin ligase inhibit binding to the proteasome. J Biol Chem. 2018;293:18387-18399 pubmed publisher
Luanpitpong S, Poohadsuan J, Samart P, Kiratipaiboon C, Rojanasakul Y, Issaragrisil S. Reactive oxygen species mediate cancer stem-like cells and determine bortezomib sensitivity via Mcl-1 and Zeb-1 in mantle cell lymphoma. Biochim Biophys Acta Mol Basis Dis. 2018;1864:3739-3753 pubmed publisher
Fauster A, Rebsamen M, Willmann K, César Razquin A, Girardi E, Bigenzahn J, et al. Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking. Cell Death Differ. 2019;26:1138-1155 pubmed publisher
Palazón Riquelme P, Worboys J, Green J, Valera A, Martín Sánchez F, Pellegrini C, et al. USP7 and USP47 deubiquitinases regulate NLRP3 inflammasome activation. EMBO Rep. 2018;19: pubmed publisher
Kato M, Placencio Hickok V, Madhav A, Haldar S, Tripathi M, Billet S, et al. Heterogeneous cancer-associated fibroblast population potentiates neuroendocrine differentiation and castrate resistance in a CD105-dependent manner. Oncogene. 2019;38:716-730 pubmed publisher
Strub T, Ghiraldini F, Carcamo S, Li M, Wroblewska A, Singh R, et al. SIRT6 haploinsufficiency induces BRAFV600E melanoma cell resistance to MAPK inhibitors via IGF signalling. Nat Commun. 2018;9:3440 pubmed publisher
Zaini M, Patel S, Syafruddin S, Rodrigues P, Vanharanta S. Endogenous HIF2A reporter systems for high-throughput functional screening. Sci Rep. 2018;8:12063 pubmed publisher
Morita K, Hama Y, Izume T, Tamura N, Ueno T, Yamashita Y, et al. Genome-wide CRISPR screen identifies TMEM41B as a gene required for autophagosome formation. J Cell Biol. 2018;217:3817-3828 pubmed publisher
Robinson McCarthy L, McCarthy K, Raaben M, Piccinotti S, Nieuwenhuis J, Stubbs S, et al. Reconstruction of the cell entry pathway of an extinct virus. PLoS Pathog. 2018;14:e1007123 pubmed publisher
Nakagawa M, Shaffer A, Ceribelli M, Zhang M, Wright G, Huang D, et al. Targeting the HTLV-I-Regulated BATF3/IRF4 Transcriptional Network in Adult T Cell Leukemia/Lymphoma. Cancer Cell. 2018;34:286-297.e10 pubmed publisher
Asada S, Goyama S, Inoue D, Shikata S, Takeda R, Fukushima T, et al. Mutant ASXL1 cooperates with BAP1 to promote myeloid leukaemogenesis. Nat Commun. 2018;9:2733 pubmed publisher
Johnson D, Taabazuing C, Okondo M, Chui A, Rao S, Brown F, et al. DPP8/DPP9 inhibitor-induced pyroptosis for treatment of acute myeloid leukemia. Nat Med. 2018;24:1151-1156 pubmed publisher
Xing Z, Zhang Y, Liang K, Yan L, Xiang Y, Li C, et al. Expression of Long Noncoding RNA YIYA Promotes Glycolysis in Breast Cancer. Cancer Res. 2018;78:4524-4532 pubmed publisher
Garri C, Howell S, Tiemann K, Tiffany A, Jalali Yazdi F, Alba M, et al. Identification, characterization and application of a new peptide against anterior gradient homolog 2 (AGR2). Oncotarget. 2018;9:27363-27379 pubmed publisher
Gan J, Dou Y, Li Y, Wang Z, Wang L, Liu S, et al. Producing anti-inflammatory macrophages by nanoparticle-triggered clustering of mannose receptors. Biomaterials. 2018;178:95-108 pubmed publisher
Oikonomidi I, Burbridge E, Cavadas M, Sullivan G, Collis B, Naegele H, et al. iTAP, a novel iRhom interactor, controls TNF secretion by policing the stability of iRhom/TACE. elife. 2018;7: pubmed publisher
García Carpizo V, Ruiz Llorente S, Sarmentero J, Graña Castro O, Pisano D, Barrero M. CREBBP/EP300 bromodomains are critical to sustain the GATA1/MYC regulatory axis in proliferation. Epigenetics Chromatin. 2018;11:30 pubmed publisher
Rodrigues P, Patel S, Harewood L, Olan I, Vojtasova E, Syafruddin S, et al. NF-κB-Dependent Lymphoid Enhancer Co-option Promotes Renal Carcinoma Metastasis. Cancer Discov. 2018;8:850-865 pubmed publisher
Tong Y, Sun J, Wong C, Kang Q, Ru B, Wong C, et al. MICMIC: identification of DNA methylation of distal regulatory regions with causal effects on tumorigenesis. Genome Biol. 2018;19:73 pubmed publisher
Klein M, Dickson M, Antonescu C, Qin L, Dooley S, Barlas A, et al. PDLIM7 and CDH18 regulate the turnover of MDM2 during CDK4/6 inhibitor therapy-induced senescence. Oncogene. 2018;37:5066-5078 pubmed publisher
Lehmer C, Schludi M, Ransom L, Greiling J, Junghänel M, Exner N, et al. A novel CHCHD10 mutation implicates a Mia40-dependent mitochondrial import deficit in ALS. EMBO Mol Med. 2018;10: pubmed publisher
Zhang R, Kim A, Fox J, Nair S, Basore K, Klimstra W, et al. Mxra8 is a receptor for multiple arthritogenic alphaviruses. Nature. 2018;557:570-574 pubmed publisher
Bowling S, Di Gregorio A, Sancho M, Pozzi S, Aarts M, Signore M, et al. P53 and mTOR signalling determine fitness selection through cell competition during early mouse embryonic development. Nat Commun. 2018;9:1763 pubmed publisher
Zheng Y, Xue M, Shen H, Li X, Ma D, Gong Y, et al. PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression. Cancer Res. 2018;78:3190-3206 pubmed publisher
Huang H, Wang C, Liu F, Li H, Peng G, Gao X, et al. Reciprocal Network between Cancer Stem-Like Cells and Macrophages Facilitates the Progression and Androgen Deprivation Therapy Resistance of Prostate Cancer. Clin Cancer Res. 2018;24:4612-4626 pubmed publisher
Anker J, Naseem A, Mok H, Schaeffer A, Abdulkadir S, Thumbikat P. Multi-faceted immunomodulatory and tissue-tropic clinical bacterial isolate potentiates prostate cancer immunotherapy. Nat Commun. 2018;9:1591 pubmed publisher
Cromwell C, Sung K, Park J, Krysler A, Jovel J, Kim S, et al. Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity. Nat Commun. 2018;9:1448 pubmed publisher
Oldrini B, Curiel García A, Marques C, Matia V, Uluçkan O, Graña Castro O, et al. Somatic genome editing with the RCAS-TVA-CRISPR-Cas9 system for precision tumor modeling. Nat Commun. 2018;9:1466 pubmed publisher
Han J, Perez J, Chen C, Li Y, Benitez A, Kandasamy M, et al. Genome-wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication. Cell Rep. 2018;23:596-607 pubmed publisher
Liu N, Hargreaves V, Zhu Q, Kurland J, Hong J, Kim W, et al. Direct Promoter Repression by BCL11A Controls the Fetal to Adult Hemoglobin Switch. Cell. 2018;173:430-442.e17 pubmed publisher
Banito A, Li X, Laporte A, Roe J, Sanchez Vega F, Huang C, et al. The SS18-SSX Oncoprotein Hijacks KDM2B-PRC1.1 to Drive Synovial Sarcoma. Cancer Cell. 2018;33:527-541.e8 pubmed publisher
Wu J, Ryskamp D, Birnbaumer L, Bezprozvanny I. Inhibition of TRPC1-Dependent Store-Operated Calcium Entry Improves Synaptic Stability and Motor Performance in a Mouse Model of Huntington's Disease. J Huntingtons Dis. 2018;7:35-50 pubmed publisher
Hill A, McFaline Figueroa J, Starita L, Gasperini M, Matreyek K, Packer J, et al. On the design of CRISPR-based single-cell molecular screens. Nat Methods. 2018;15:271-274 pubmed publisher
Ringer K, Roth M, Garey M, Piorczynski T, Suli A, Hansen J, et al. Comparative analysis of lipid-mediated CRISPR-Cas9 genome editing techniques. Cell Biol Int. 2018;42:849-858 pubmed publisher
Sykora P, Witt K, Revanna P, Smith Roe S, Dismukes J, Lloyd D, et al. Next generation high throughput DNA damage detection platform for genotoxic compound screening. Sci Rep. 2018;8:2771 pubmed publisher
Okondo M, Rao S, Taabazuing C, Chui A, Poplawski S, Johnson D, et al. Inhibition of Dpp8/9 Activates the Nlrp1b Inflammasome. Cell Chem Biol. 2018;25:262-267.e5 pubmed publisher
Zeid R, Lawlor M, Poon E, Reyes J, Fulciniti M, Lopez M, et al. Enhancer invasion shapes MYCN-dependent transcriptional amplification in neuroblastoma. Nat Genet. 2018;50:515-523 pubmed publisher
Thu K, Silvester J, Elliott M, Ba Alawi W, Duncan M, Elia A, et al. Disruption of the anaphase-promoting complex confers resistance to TTK inhibitors in triple-negative breast cancer. Proc Natl Acad Sci U S A. 2018;115:E1570-E1577 pubmed publisher
Yin H, Song C, Suresh S, Kwan S, Wu Q, Walsh S, et al. Partial DNA-guided Cas9 enables genome editing with reduced off-target activity. Nat Chem Biol. 2018;14:311-316 pubmed publisher
Alam M, Gaida M, Debnath S, Tagad H, Miller Jenkins L, Appella E, et al. Unique properties of TCR-activated p38 are necessary for NFAT-dependent T-cell activation. PLoS Biol. 2018;16:e2004111 pubmed publisher
Lagrange B, Benaoudia S, Wallet P, Magnotti F, Provost A, Michal F, et al. Human caspase-4 detects tetra-acylated LPS and cytosolic Francisella and functions differently from murine caspase-11. Nat Commun. 2018;9:242 pubmed publisher
Jiang S, Wang L, Walsh M, Trudeau S, Gerdt C, Zhao B, et al. CRISPR/Cas9-Mediated Genome Editing in Epstein-Barr Virus-Transformed Lymphoblastoid B-Cell Lines. Curr Protoc Mol Biol. 2018;121:31.12.1-31.12.23 pubmed publisher
Seino T, Kawasaki S, Shimokawa M, Tamagawa H, Toshimitsu K, Fujii M, et al. Human Pancreatic Tumor Organoids Reveal Loss of Stem Cell Niche Factor Dependence during Disease Progression. Cell Stem Cell. 2018;22:454-467.e6 pubmed publisher
Song W, Joshita S, Zhou Y, Ulland T, Gilfillan S, Colonna M. Humanized TREM2 mice reveal microglia-intrinsic and -extrinsic effects of R47H polymorphism. J Exp Med. 2018;215:745-760 pubmed publisher
Gwinn D, Lee A, Briones Martin del Campo M, Conn C, Simpson D, Scott A, et al. Oncogenic KRAS Regulates Amino Acid Homeostasis and Asparagine Biosynthesis via ATF4 and Alters Sensitivity to L-Asparaginase. Cancer Cell. 2018;33:91-107.e6 pubmed publisher
Pusapati G, Kong J, Patel B, Krishnan A, Sagner A, Kinnebrew M, et al. CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog. Dev Cell. 2018;44:113-129.e8 pubmed publisher
Nitulescu I, Meyer S, Wen Q, Crispino J, Lemieux M, Levine R, et al. Mediator Kinase Phosphorylation of STAT1 S727 Promotes Growth of Neoplasms With JAK-STAT Activation. EBioMedicine. 2017;26:112-125 pubmed publisher
Barrett S, Parker K, Horn C, Mata M, Salzman J. ciRS-7 exonic sequence is embedded in a long non-coding RNA locus. PLoS Genet. 2017;13:e1007114 pubmed publisher
Yu R, Longo J, van Leeuwen J, Mullen P, Ba Alawi W, Haibe Kains B, et al. Statin-Induced Cancer Cell Death Can Be Mechanistically Uncoupled from Prenylation of RAS Family Proteins. Cancer Res. 2018;78:1347-1357 pubmed publisher
Zhao G, Wang Q, Gu Q, Qiang W, Wei J, Dong P, et al. Lentiviral CRISPR/Cas9 nickase vector mediated BIRC5 editing inhibits epithelial to mesenchymal transition in ovarian cancer cells. Oncotarget. 2017;8:94666-94680 pubmed publisher
Shukla S, Cyrta J, Murphy D, Walczak E, Ran L, Agrawal P, et al. Aberrant Activation of a Gastrointestinal Transcriptional Circuit in Prostate Cancer Mediates Castration Resistance. Cancer Cell. 2017;32:792-806.e7 pubmed publisher
Fontanals Cirera B, Hasson D, Vardabasso C, Di Micco R, Agrawal P, Chowdhury A, et al. Harnessing BET Inhibitor Sensitivity Reveals AMIGO2 as a Melanoma Survival Gene. Mol Cell. 2017;68:731-744.e9 pubmed publisher
Brown J, Yonekubo Y, Hanson N, Sastre Perona A, Basin A, Rytlewski J, et al. TGF-β-Induced Quiescence Mediates Chemoresistance of Tumor-Propagating Cells in Squamous Cell Carcinoma. Cell Stem Cell. 2017;21:650-664.e8 pubmed publisher
Vinjamur D, Bauer D. Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines. Methods Mol Biol. 2018;1698:275-284 pubmed publisher
Goyal A, Fiskin E, Gutschner T, Polycarpou Schwarz M, Groß M, Neugebauer J, et al. A cautionary tale of sense-antisense gene pairs: independent regulation despite inverse correlation of expression. Nucleic Acids Res. 2017;45:12496-12508 pubmed publisher
Jiang S, Zhou H, Liang J, Gerdt C, Wang C, Ke L, et al. The Epstein-Barr Virus Regulome in Lymphoblastoid Cells. Cell Host Microbe. 2017;22:561-573.e4 pubmed publisher
Reddy A, Zhang J, Davis N, Moffitt A, Love C, Waldrop A, et al. Genetic and Functional Drivers of Diffuse Large B Cell Lymphoma. Cell. 2017;171:481-494.e15 pubmed publisher
Yau E, Kummetha I, Lichinchi G, Tang R, Zhang Y, Rana T. Genome-Wide CRISPR Screen for Essential Cell Growth Mediators in Mutant KRAS Colorectal Cancers. Cancer Res. 2017;77:6330-6339 pubmed publisher
Jiang S, Xu W, Emery A, Gerfen C, Eiden M, Eiden L. NCS-Rapgef2, the Protein Product of the Neuronal Rapgef2 Gene, Is a Specific Activator of D1 Dopamine Receptor-Dependent ERK Phosphorylation in Mouse Brain. Eneuro. 2017;4: pubmed publisher
Zhao N, Wang G, Das A, Berkhout B. Combinatorial CRISPR-Cas9 and RNA Interference Attack on HIV-1 DNA and RNA Can Lead to Cross-Resistance. Antimicrob Agents Chemother. 2017;61: pubmed publisher
Luanpitpong S, Angsutararux P, Samart P, Chanthra N, Chanvorachote P, Issaragrisil S. Hyper-O-GlcNAcylation induces cisplatin resistance via regulation of p53 and c-Myc in human lung carcinoma. Sci Rep. 2017;7:10607 pubmed publisher
Napier B, Monack D. Creating a RAW264.7 CRISPR-Cas9 Genome Wide Library. Bio Protoc. 2017;7: pubmed publisher
Samwer M, Schneider M, Hoefler R, Schmalhorst P, Jude J, Zuber J, et al. DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes. Cell. 2017;170:956-972.e23 pubmed publisher
Kim J, Abudayyeh O, Yeerna H, Yeang C, Stewart M, Jenkins R, et al. Decomposing Oncogenic Transcriptional Signatures to Generate Maps of Divergent Cellular States. Cell Syst. 2017;5:105-118.e9 pubmed publisher
Stewart S, Menzies S, Popa S, Savinykh N, Petrunkina Harrison A, Lehner P, et al. A genome-wide CRISPR screen reconciles the role of N-linked glycosylation in galectin-3 transport to the cell surface. J Cell Sci. 2017;130:3234-3247 pubmed publisher
Harding S, Benci J, Irianto J, Discher D, Minn A, Greenberg R. Mitotic progression following DNA damage enables pattern recognition within micronuclei. Nature. 2017;548:466-470 pubmed publisher
Konitsiotis A, Roßmannek L, Stanoev A, Schmick M, Bastiaens P. Spatial cycles mediated by UNC119 solubilisation maintain Src family kinases plasma membrane localisation. Nat Commun. 2017;8:114 pubmed publisher
Erard N, Knott S, Hannon G. A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout. Mol Cell. 2017;67:348-354.e4 pubmed publisher
Lessard S, Gatof E, Beaudoin M, Schupp P, Sher F, Ali A, et al. An erythroid-specific ATP2B4 enhancer mediates red blood cell hydration and malaria susceptibility. J Clin Invest. 2017;127:3065-3074 pubmed publisher
Gasperini M, Findlay G, McKenna A, Milbank J, Lee C, Zhang M, et al. CRISPR/Cas9-Mediated Scanning for Regulatory Elements Required for HPRT1 Expression via Thousands of Large, Programmed Genomic Deletions. Am J Hum Genet. 2017;101:192-205 pubmed publisher
Zabala Letona A, Arruabarrena Aristorena A, Martin Martin N, Fernandez Ruiz S, Sutherland J, Clasquin M, et al. mTORC1-dependent AMD1 regulation sustains polyamine metabolism in prostate cancer. Nature. 2017;547:109-113 pubmed publisher
Gaytan Cervantes J, Gonzalez Torres C, Maldonado V, Zampedri C, Ceballos Cancino G, Melendez Zajgla J. Protein Sam68 regulates the alternative splicing of survivin DEx3. J Biol Chem. 2017;292:13745-13757 pubmed publisher
Miles L, Burga L, Gardner E, Bostina M, Poirier J, Rudin C. Anthrax toxin receptor 1 is the cellular receptor for Seneca Valley virus. J Clin Invest. 2017;127:2957-2967 pubmed publisher
Kawazu M, Kojima S, Ueno T, Totoki Y, Nakamura H, Kunita A, et al. Integrative analysis of genomic alterations in triple-negative breast cancer in association with homologous recombination deficiency. PLoS Genet. 2017;13:e1006853 pubmed publisher
Deb Roy A, Yin T, Choudhary S, Rodionov V, Pilbeam C, Wu Y. Optogenetic activation of Plexin-B1 reveals contact repulsion between osteoclasts and osteoblasts. Nat Commun. 2017;8:15831 pubmed publisher
Mou H, Smith J, Peng L, Yin H, Moore J, Zhang X, et al. CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion. Genome Biol. 2017;18:108 pubmed publisher
Jaiswal A, Peddinti G, Akimov Y, Wennerberg K, Kuznetsov S, Tang J, et al. Seed-effect modeling improves the consistency of genome-wide loss-of-function screens and identifies synthetic lethal vulnerabilities in cancer cells. Genome Med. 2017;9:51 pubmed publisher
Li Q, Li Y, Xu J, Wang S, Xu Y, Li X, et al. Aldolase B Overexpression is Associated with Poor Prognosis and Promotes Tumor Progression by Epithelial-Mesenchymal Transition in Colorectal Adenocarcinoma. Cell Physiol Biochem. 2017;42:397-406 pubmed publisher
Chapel A, Garcia Beltran W, Hölzemer A, Ziegler M, Lunemann S, Martrus G, et al. Peptide-specific engagement of the activating NK cell receptor KIR2DS1. Sci Rep. 2017;7:2414 pubmed publisher
Yonezawa T, Takahashi H, Shikata S, Liu X, Tamura M, Asada S, et al. The ubiquitin ligase STUB1 regulates stability and activity of RUNX1 and RUNX1-RUNX1T1. J Biol Chem. 2017;292:12528-12541 pubmed publisher
Petris G, Casini A, Montagna C, Lorenzin F, Prandi D, Romanel A, et al. Hit and go CAS9 delivered through a lentiviral based self-limiting circuit. Nat Commun. 2017;8:15334 pubmed publisher
Ma Y, Walsh M, Bernhardt K, Ashbaugh C, Trudeau S, Ashbaugh I, et al. CRISPR/Cas9 Screens Reveal Epstein-Barr Virus-Transformed B Cell Host Dependency Factors. Cell Host Microbe. 2017;21:580-591.e7 pubmed publisher
Hodonsky C, Jain D, Schick U, Morrison J, Brown L, McHugh C, et al. Genome-wide association study of red blood cell traits in Hispanics/Latinos: The Hispanic Community Health Study/Study of Latinos. PLoS Genet. 2017;13:e1006760 pubmed publisher
Strezoska Z, Perkett M, Chou E, Maksimova E, Anderson E, McClelland S, et al. High-content analysis screening for cell cycle regulators using arrayed synthetic crRNA libraries. J Biotechnol. 2017;251:189-200 pubmed publisher
Hung V, Lam S, Udeshi N, Svinkina T, Guzman G, Mootha V, et al. Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation. elife. 2017;6: pubmed publisher
Betts R, Perkovic A, Mahapatra S, Del Bufalo A, Camara K, Howell A, et al. Contact sensitizers trigger human CD1-autoreactive T-cell responses. Eur J Immunol. 2017;47:1171-1180 pubmed publisher
Taabazuing C, Okondo M, Bachovchin D. Pyroptosis and Apoptosis Pathways Engage in Bidirectional Crosstalk in Monocytes and Macrophages. Cell Chem Biol. 2017;24:507-514.e4 pubmed publisher
Besse W, Dong K, Choi J, Punia S, Fedeles S, Choi M, et al. Isolated polycystic liver disease genes define effectors of polycystin-1 function. J Clin Invest. 2017;127:1772-1785 pubmed publisher
Li X, Song N, Liu L, Liu X, Ding X, Song X, et al. USP9X regulates centrosome duplication and promotes breast carcinogenesis. Nat Commun. 2017;8:14866 pubmed publisher
Joung J, Konermann S, Gootenberg J, Abudayyeh O, Platt R, Brigham M, et al. Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Nat Protoc. 2017;12:828-863 pubmed publisher
Iurlaro R, Püschel F, León Annicchiarico C, O Connor H, Martin S, Palou Gramón D, et al. Glucose Deprivation Induces ATF4-Mediated Apoptosis through TRAIL Death Receptors. Mol Cell Biol. 2017;37: pubmed publisher
Drané P, Brault M, Cui G, Meghani K, Chaubey S, Detappe A, et al. TIRR regulates 53BP1 by masking its histone methyl-lysine binding function. Nature. 2017;543:211-216 pubmed publisher
Ohlund D, Handly Santana A, Biffi G, Elyada E, Almeida A, Ponz Sarvisé M, et al. Distinct populations of inflammatory fibroblasts and myofibroblasts in pancreatic cancer. J Exp Med. 2017;214:579-596 pubmed publisher
Canver M, Lessard S, Pinello L, Wu Y, Ilboudo Y, Stern E, et al. Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci. Nat Genet. 2017;49:625-634 pubmed publisher
García Tuñón I, Hernández Sánchez M, Ordoñez J, Alonso Pérez V, Álamo Quijada M, Benito R, et al. The CRISPR/Cas9 system efficiently reverts the tumorigenic ability of BCR/ABL in vitro and in a xenograft model of chronic myeloid leukemia. Oncotarget. 2017;8:26027-26040 pubmed publisher
Goyal A, Myacheva K, Groß M, Klingenberg M, Duran Arqué B, Diederichs S. Challenges of CRISPR/Cas9 applications for long non-coding RNA genes. Nucleic Acids Res. 2017;45:e12 pubmed publisher
Taniguchi K, Moroishi T, de Jong P, Krawczyk M, Grebbin B, Luo H, et al. YAP-IL-6ST autoregulatory loop activated on APC loss controls colonic tumorigenesis. Proc Natl Acad Sci U S A. 2017;114:1643-1648 pubmed publisher
Datlinger P, Rendeiro A, Schmidl C, Krausgruber T, Traxler P, Klughammer J, et al. Pooled CRISPR screening with single-cell transcriptome readout. Nat Methods. 2017;14:297-301 pubmed publisher
Kim H, Song M, Lee J, Menon A, Jung S, Kang Y, et al. In vivo high-throughput profiling of CRISPR-Cpf1 activity. Nat Methods. 2017;14:153-159 pubmed publisher
Wang G, Zhao N, Berkhout B, Das A. A Combinatorial CRISPR-Cas9 Attack on HIV-1 DNA Extinguishes All Infectious Provirus in Infected T Cell Cultures. Cell Rep. 2016;17:2819-2826 pubmed publisher
Yamauchi S, Takeuchi K, Chihara K, Honjoh C, Kato Y, Yoshiki H, et al. STAT1 is essential for the inhibition of hepatitis C virus replication by interferon-λ but not by interferon-α. Sci Rep. 2016;6:38336 pubmed publisher
Okondo M, Johnson D, Sridharan R, Go E, Chui A, Wang M, et al. DPP8 and DPP9 inhibition induces pro-caspase-1-dependent monocyte and macrophage pyroptosis. Nat Chem Biol. 2017;13:46-53 pubmed publisher
Ryskamp D, Wu J, Geva M, Kusko R, Grossman I, Hayden M, et al. The sigma-1 receptor mediates the beneficial effects of pridopidine in a mouse model of Huntington disease. Neurobiol Dis. 2017;97:46-59 pubmed publisher
Haga K, Fujimoto A, Takai Todaka R, Miki M, Doan Y, Murakami K, et al. Functional receptor molecules CD300lf and CD300ld within the CD300 family enable murine noroviruses to infect cells. Proc Natl Acad Sci U S A. 2016;113:E6248-E6255 pubmed
Tao L, Zhang J, Meraner P, Tovaglieri A, Wu X, Gerhard R, et al. Frizzled proteins are colonic epithelial receptors for C. difficile toxin B. Nature. 2016;538:350-355 pubmed publisher
Bailey S, Desai K, Kron K, Mazrooei P, Sinnott Armstrong N, Treloar A, et al. Noncoding somatic and inherited single-nucleotide variants converge to promote ESR1 expression in breast cancer. Nat Genet. 2016;48:1260-6 pubmed publisher
Yi Z, Fang C, Zou J, Xu J, Song W, Du X, et al. Affinity Purification of the Hepatitis C Virus Replicase Identifies Valosin-Containing Protein, a Member of the ATPases Associated with Diverse Cellular Activities Family, as an Active Virus Replication Modulator. J Virol. 2016;90:9953-9966 pubmed publisher
Kaminski R, Chen Y, Salkind J, Bella R, Young W, Ferrante P, et al. Negative Feedback Regulation of HIV-1 by Gene Editing Strategy. Sci Rep. 2016;6:31527 pubmed publisher
Comte D, Karampetsou M, Kis Toth K, Yoshida N, Bradley S, Mizui M, et al. Engagement of SLAMF3 enhances CD4+ T-cell sensitivity to IL-2 and favors regulatory T-cell polarization in systemic lupus erythematosus. Proc Natl Acad Sci U S A. 2016;113:9321-6 pubmed publisher
Garcia Beltran W, Hölzemer A, Martrus G, Chung A, Pacheco Y, Simoneau C, et al. Open conformers of HLA-F are high-affinity ligands of the activating NK-cell receptor KIR3DS1. Nat Immunol. 2016;17:1067-74 pubmed publisher
Lambrus B, Daggubati V, Uetake Y, Scott P, Clutario K, Sluder G, et al. A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis. J Cell Biol. 2016;214:143-53 pubmed publisher
Zhang R, Miner J, Gorman M, Rausch K, Ramage H, White J, et al. A CRISPR screen defines a signal peptide processing pathway required by flaviviruses. Nature. 2016;535:164-8 pubmed
McDade J, Waxmonsky N, Swanson L, Fan M. Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Curr Protoc Mol Biol. 2016;115:31.5.1-31.5.13 pubmed publisher
Karlas A, Berrè S, Couderc T, Varjak M, Braun P, Meyer M, et al. A human genome-wide loss-of-function screen identifies effective chikungunya antiviral drugs. Nat Commun. 2016;7:11320 pubmed publisher
Yin H, Song C, Dorkin J, Zhu L, Li Y, Wu Q, et al. Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo. Nat Biotechnol. 2016;34:328-33 pubmed publisher
Wang G, Zhao N, Berkhout B, Das A. CRISPR-Cas9 Can Inhibit HIV-1 Replication but NHEJ Repair Facilitates Virus Escape. Mol Ther. 2016;24:522-6 pubmed publisher
Wu J, Ryskamp D, Liang X, Egorova P, Zakharova O, Hung G, et al. Enhanced Store-Operated Calcium Entry Leads to Striatal Synaptic Loss in a Huntington's Disease Mouse Model. J Neurosci. 2016;36:125-41 pubmed publisher
Dang Y, Jia G, Choi J, Ma H, Anaya E, Ye C, et al. Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency. Genome Biol. 2015;16:280 pubmed publisher
Zhang X, Choi P, FRANCIS J, Imielinski M, Watanabe H, Cherniack A, et al. Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers. Nat Genet. 2016;48:176-82 pubmed publisher
Hunter J, Butterworth J, Zhao B, Sellier H, Campbell K, Thomas H, et al. The NF-κB subunit c-Rel regulates Bach2 tumour suppressor expression in B-cell lymphoma. Oncogene. 2016;35:3476-84 pubmed publisher
Aparicio Prat E, Arnan C, Sala I, Bosch N, Guigó R, Johnson R. DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs. BMC Genomics. 2015;16:846 pubmed publisher
Canver M, SMITH E, Sher F, Pinello L, Sanjana N, Shalem O, et al. BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis. Nature. 2015;527:192-7 pubmed publisher
Hayashi M, Cesare A, Rivera T, Karlseder J. Cell death during crisis is mediated by mitotic telomere deprotection. Nature. 2015;522:492-6 pubmed publisher
Greenfeld H, Takasaki K, Walsh M, Ersing I, Bernhardt K, Ma Y, et al. TRAF1 Coordinates Polyubiquitin Signaling to Enhance Epstein-Barr Virus LMP1-Mediated Growth and Survival Pathway Activation. PLoS Pathog. 2015;11:e1004890 pubmed publisher
Doench J, Hartenian E, Graham D, Tothova Z, Hegde M, Smith I, et al. Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Nat Biotechnol. 2014;32:1262-7 pubmed publisher
Sanjana N, Shalem O, Zhang F. Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014;11:783-784 pubmed publisher
product information
Catalog Number :
52962
Product Name :
lentiCas9-Blast
article :
doi10.1038/nmeth.3047
id9103
pubmed_id25075903
bacterial resistance :
Ampicillin
cloning :
backbonepFUGW
backbone_mutation
backbone_origin
backbone_size10130
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
growth notes :
Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check https://zlab.bio/ for the most up-to-date information.
growth strain :
Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.
growth temp :
Use SapI digest to check for unwanted recombination of lentiviral plasmid. Only amplify in RecA- bacteria (eg. Stbl3).
origin :
37
pi :
alt_names
S. pyogenes CRISPR-Cas9
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5AgeI, XbaI, AfeI
promoterEFS-NS
sequencing_primer_3blast-R (5'-gctctttcaatgagggtgga-3' )
sequencing_primer_5pLLU-F (5'-gggacagcagagatccagtt-3')
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameCas9
shRNA_sequence
size4200
species
100
Synthetic
tags
locationC terminal on insert
tagFLAG
alt_names
blasticidin S deaminase
bsd
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoterEFS-NS
sequencing_primer_3WPRE-R
sequencing_primer_5needs custom
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameBlasticidin resistance
shRNA_sequence
size399
species
tags
resistance markers :
898
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA