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product summary
company name :
Addgene
product type :
cDNA
product name :
lentiCRISPR v2
catalog :
52961
citations: 2119
Reference
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product information
Catalog Number :
52961
Product Name :
lentiCRISPR v2
article :
doi10.1038/nmeth.3047
id9103
pubmed_id25075903
bacterial resistance :
Ampicillin
cloning :
backboneCustom
backbone_mutation
backbone_origin
backbone_size10000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
growth notes :
This plasmid is an updated version of the original lentiCRISPR (Addgene plasmid #49535) IMPORTANT: The primers suggestions listed above are for gene inserts that exist in the untouched vector. After you have inserted your gRNA, you should use hU6-F (5'-GAGGGCCTATTTCCCATGATT-3') or LKO.1 5 (5'- GACTATCATATGCTTACCGT-3') to sequence that region. Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check https://zlab.bio/ for the most up-to-date information.
growth strain :
Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
growth temp :
Use SapI digest to check for unwanted recombination of lentiviral plasmid. Only amplify in RecA- bacteria (eg. Stbl3).
origin :
37
pi :
alt_names
S. pyogenes CRISPR-Cas9
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5AgeI, XbaI, AfeI
promoterEFS-NS
sequencing_primer_3WPRE-R
sequencing_primer_5hEF1aprom-F
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameCas9
shRNA_sequence
size4200
species
100
Synthetic
tags
locationC terminal on insert
tagFLAG
alt_names
puromycin N-acetyl-transferase
PAC
cloning
clone_methodRestriction Enzyme
cloning_site_3MluI
cloning_site_5BamHI
promoterEFS-NS
sequencing_primer_3WPRE-R
sequencing_primer_5Puro-F2
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namePuromycin resistance
shRNA_sequence
size600
species
tags
resistance markers :
898
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA