pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB | Addgene 52890 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

catalog :

52890

citations: 2

Reference
Quintero M, Bangi E. Disruptions in cell fate decisions and transformed enteroendocrine cells drive intestinal tumorigenesis in Drosophila. Cell Rep. 2023;42:113370 pubmed publisher
Bosch J, Tran N, Hariharan I. CoinFLP: a system for efficient mosaic screening and for visualizing clonal boundaries in Drosophila. Development. 2015;142:597-606 pubmed publisher

product information

Catalog Number :

52890

Product Name :

pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

article :

doi	10.1242/dev.114603
id	9641
pubmed_id	25605786

bacterial resistance :

Ampicillin

cloning :

backbone

pAct5-FRT-stop-FRT-SV40polyA attB

backbone_mutation

backbone_origin

David Strutt (Brittle et al., 2010)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

growth strain :

CoinFLP-LexGAD/Gal4 - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.

origin :

pi :

alt_names

nlsLexA::GADfl

cloning

entrez_gene

genbank_ids

mutation

name

LexGAD

shRNA_sequence

size

2853

species

4932

Saccharomyces cerevisiae

32644

Other

E. coli