pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB | Addgene 52889 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB

catalog :

52889

citations: 3

Reference
Ray A, Li X. A Notch-dependent transcriptional mechanism controls expression of temporal patterning factors in Drosophila medulla. elife. 2022;11: pubmed publisher
Patel A, Wu Y, Han X, Su Y, Maugel T, Shroff H, et al. Cytonemes coordinate asymmetric signaling and organization in the Drosophila muscle progenitor niche. Nat Commun. 2022;13:1185 pubmed publisher
Bosch J, Tran N, Hariharan I. CoinFLP: a system for efficient mosaic screening and for visualizing clonal boundaries in Drosophila. Development. 2015;142:597-606 pubmed publisher

product information

Catalog Number :

52889

Product Name :

pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB

article :

doi	10.1242/dev.114603
id	9641
pubmed_id	25605786

bacterial resistance :

Ampicillin

cloning :

backbone

pAct5-FRT-stop-FRT-SV40polyA attB

backbone_mutation

backbone_origin

David Strutt (Brittle et al., 2010)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

growth strain :

CoinFLP-Gal4 - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression

origin :

pi :

alt_names

cloning

entrez_gene

aliases	YPL248C, GAL81
gene	GAL4
id	855828

genbank_ids

mutation

name

Gal4

shRNA_sequence

size

species

4932

Saccharomyces cerevisiae