This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pB-GLUT4-7myc-GFP
catalog :
52872
citations: 8
Reference
Knudsen J, Persson K, Henríquez Olguín C, Li Z, Di Leo N, Hesselager S, et al. Microtubule-mediated GLUT4 trafficking is disrupted in insulin-resistant skeletal muscle. elife. 2023;12: pubmed publisher
Song N, Lee A, Yasmeen R, Shen Q, Yang K, Kumar S, et al. Epiregulin as an Alternative Ligand for Leptin Receptor Alleviates Glucose Intolerance without Change in Obesity. Cells. 2022;11: pubmed publisher
Pessoa Rodrigues C, Chatterjee A, Wiese M, Stehle T, Szymanski W, Shvedunova M, et al. Histone H4 lysine 16 acetylation controls central carbon metabolism and diet-induced obesity in mice. Nat Commun. 2021;12:6212 pubmed publisher
Gu J, Zhang W, Wu L, Gu Y. CFTR Deficiency Affects Glucose Homeostasis via Regulating GLUT4 Plasma Membrane Transportation. Front Cell Dev Biol. 2021;9:630654 pubmed publisher
Das P, Park M, Lee C, Nam S, Kim Y, Kim D, et al. Aqueous green tea infusion extracted by ultra-sonication method, but not by conventional method, facilitates GLUT4 membrane translocation in adipocytes which potently ameliorates high-fat diet-induced obesity. J Food Biochem. 2021;45:e13561 pubmed publisher
Alshafie W, Francis V, Bednarz K, Pan Y, Stroh T, McPherson P. Regulated resurfacing of a somatostatin receptor storage compartment fine-tunes pituitary secretion. J Cell Biol. 2020;219: pubmed publisher
Henríquez Olguín C, Knudsen J, Raun S, Li Z, Dalbram E, Treebak J, et al. Cytosolic ROS production by NADPH oxidase 2 regulates muscle glucose uptake during exercise. Nat Commun. 2019;10:4623 pubmed publisher
Bogan J, McKee A, Lodish H. Insulin-responsive compartments containing GLUT4 in 3T3-L1 and CHO cells: regulation by amino acid concentrations. Mol Cell Biol. 2001;21:4785-806 pubmed
product information
Catalog Number :
52872
Product Name :
pB-GLUT4-7myc-GFP
article :
doi10.1128/MCB.21.14.4785-4806.2001
id8190
pubmed_id11416153
bacterial resistance :
Ampicillin
cloning :
backbonepB
backbone_mutationThe vector is based on pMX except that it contains mutations (ATG to GTG) at 1564 and at 1072 to eliminate potential start sites upstream of the cloning site.
backbone_originBogan lab, unpublished
backbone_size5783
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Plasmid is also called pB-G7. We use FACS to select infected cells that express GFP. There is a short linker between GLUT4 and GFP.
growth strain :
detection and measurement of GLUT4 translocation
origin :
37
pi :
alt_names
SLC2A4
solute carrier family 2
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5EcoRI
promoter
sequencing_primer_3MX2 CCCCTTTTTCTGGAGACTAAAT
sequencing_primer_5MX1 CCACCGCCCTCAAAGTAGACG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGlut4
geneSlc2a4
id25139
genbank_ids
mutation
nameGLUT4
shRNA_sequence
size
species
10116
Rattus norvegicus
32644
Other
with human c-terminus
tags
locationN terminal on insert
tag7x-myc (internal)
locationC terminal on insert
tagGFP
resistance markers :
1915
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA