This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET28-Kss1
catalog :
52682
citations: 1
Reference
Mok J, Kim P, Lam H, Piccirillo S, Zhou X, Jeschke G, et al. Deciphering protein kinase specificity through large-scale analysis of yeast phosphorylation site motifs. Sci Signal. 2010;3:ra12 pubmed publisher
product information
Catalog Number :
52682
Product Name :
pET28-Kss1
article :
doi10.1126/scisignal.2000482
id8168
pubmed_id20159853
bacterial resistance :
Kanamycin
cloning :
backbonepET28a
backbone_mutation
backbone_originEMD Biosciences
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
When co-expressed with Ste7EE (addgene plasmid #52681), produces active phosphorylated Kss1.
growth strain :
Expression of Kss1 in bacteria
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoterT7
sequencing_primer_3T7 terminal
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYGR040W
geneKSS1
id852931
genbank_ids
852931
mutation
nameKSS1
shRNA_sequence
size1100
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on backbone
tagHis6
locationN terminal on backbone
tagT7 tag
resistance markers :
1910
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA