This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX4T2-Ste7-EE
catalog :
52681
citations: 1
Reference
Mok J, Kim P, Lam H, Piccirillo S, Zhou X, Jeschke G, et al. Deciphering protein kinase specificity through large-scale analysis of yeast phosphorylation site motifs. Sci Signal. 2010;3:ra12 pubmed publisher
product information
Catalog Number :
52681
Product Name :
pGEX4T2-Ste7-EE
article :
doi10.1126/scisignal.2000482
id8168
pubmed_id20159853
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-4T2
backbone_mutation
backbone_originGE Healthcare Life Sciences
backbone_size4900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
A F2V mutation was discovered, it should not effect the function of the plasmid.
growth strain :
expression of constitutively active Ste7 kinase in bacteria
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BamHI
promotertac
sequencing_primer_3pGEX3'
sequencing_primer_5pGEX5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYDL159W
geneSTE7
id851396
genbank_ids
851396
mutationS359E/T363E constitutively active
nameSte7
shRNA_sequence
size1545
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on backbone
tagGST
locationC terminal on insert
tagMyc
plasmid copy :
Beverly Errede (please see Maleri et al, Mol Cell Biol. 2004 Oct;24(20):9221-38. PubMed: 15456892 )
resistance markers :
1910
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA