pSIL-eGFP-shACTN4 | Addgene 52679 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSIL-eGFP-shACTN4

catalog :

52679

citations: 1

Reference
Schnizler M, Schnizler K, Zha X, Hall D, Wemmie J, Hell J, et al. The cytoskeletal protein alpha-actinin regulates acid-sensing ion channel 1a through a C-terminal interaction. J Biol Chem. 2009;284:2697-705 pubmed publisher

product information

Catalog Number :

52679

Product Name :

pSIL-eGFP-shACTN4

article :

doi	10.1074/jbc.M805110200
id	8166
pubmed_id	19028690

bacterial resistance :

Ampicillin

cloning :

backbone

pSIL-EGFP

backbone_mutation

backbone_origin

Hell lab, Addgene plasmid #52675

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

RNAi

growth notes :

. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3 end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer vector for expression of the respective sequences under the U6 promotor.
-gactatccaggagatgcagttcaagagactgcatctcc
tggatagtctttttt-3
EGFP is a transfection marker, not a fusion. RNAi targeted at 493 511 from the start sequence of rat -actinin-4 (the sense chain was 5

growth strain :

shRNA against -actinin-4 with eGFP transfection marker

origin :

pi :

norvegicustags

location	N terminal on backbone
tag	CMV-EGFP

4cl
oning>promoterr>

clone_meth od	Unknown
clo ning_site_3
c loning_site_5
U6
s equencing_primer_3	M13R
sequencing_primer_5	E GFP-C
site_3_destroyed
site_5_destr oyed

r>entrez_gener>

aliases	td>
gene	Actn4
id	63836

>genbank_ids
mutation
nameACTN4shR
NA_sequencegactatccaggagatgcag
ttcaagagactgcatctcctggatagtctttttt
size>species

ble>

10116

Ratt
us

alt_names

actinin, alpha

resistance markers :

1913

tags :

Unknown