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product summary
company name :
Addgene
product type :
cDNA
product name :
pSIL-eGFP-shACTN3
catalog :
52678
citations: 1
product information
Catalog Number :
52678
Product Name :
pSIL-eGFP-shACTN3
article :
| doi | 10.1074/jbc.M805110200 |
| id | 8166 |
| pubmed_id | 19028690 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pSIL-EGFP | |
| backbone_mutation | ||
| backbone_origin | Hell lab, Addgene plasmid #52675 | |
| backbone_size | ||
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3 end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer vector for expression of the respective sequences under the U6 promotor.
-aacgagaagctgatggaggttcaagagacctccatcag
cttctcgtttttttt-3
EGFP is a transfection marker, not a fusion. RNAi targeted at 455 473 from the start sequence of rat -actinin-3 (the sense chain was 5
-aacgagaagctgatggaggttcaagagacctccatcag
cttctcgtttttttt-3
EGFP is a transfection marker, not a fusion. RNAi targeted at 455 473 from the start sequence of rat -actinin-3 (the sense chain was 5
growth strain :
shRNA against -actinin-3 with eGFP transfection marker
origin :
37
pi :
norvegicustags
3cl
oning
entrez_gene
r>genbank_ids >mutation >nameACTN3 sh
RNA_sequence aacgagaagctgatggag
gttcaagagacctccatcagcttctcgtttttttt> size r>species
|
3
oning
| clone_meth od | Unknown |
| clo ning_site_3 | |
| c loning_site_5 | |
| U6 | |
| s equencing_primer_3 | M13R | r>
| sequencing_primer_5 | E GFP-C |
| site_3_destroyed | |
| site_5_destr oyed |
|
RNA_sequence
gttcaagagacctccatcagcttctcgtttttttt>
| 10116 | Rat | tus
|