This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PSD-95_Y12E-pTagRFP
catalog :
52672
product information
Catalog Number :
52672
Product Name :
PSD-95_Y12E-pTagRFP
article :
doi
id8165
pubmed_id
bacterial resistance :
Kanamycin
cloning :
backbonepTagRFP
backbone_mutation
backbone_originEvrogen
backbone_size4733
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
HindII and EcoRI were used to cut PSD-95 from PSD-95-pGW1 (received from David Bredt) and ligate it into pTagRFP-N. PSD-95 tyrosine 12 was mutated to glutamate (Y12E) by extending a complementary set of primers (PSD95Y12E-forward: 5 -GAC AAC CAA GAA AGA GCG CTA CCA AGA TGA AGA CAC G-3 , PSD95Y12E-reverse: 5'-CGT GTC TTC ATC TTG GTA GCG CTC TTT CTT GGT TGT C-3') along the whole template plasmid using Q5 high-fidelity DNA polymerase (NEB). DpnI was subsequently used to digest non-mutated, methylated template DNA prior to transformation.
growth strain :
expression of TagRFP-tagged PSD-95_Y12E
origin :
37
pi :
alt_names
Postsynaptic density protein 95
Disks large homolog 4 (DLG4)
Synapse-associated protein 90 (SAP-90)
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3mKate2-TurboRFP-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDlgh4, PSD95, Sap90
geneDlg4
id29495
genbank_ids
P78352
mutationY12E
namePSD-95
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationC terminal on backbone
tagtagRFP
resistance markers :
1913
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA