This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
ACTN3-pEGFP
catalog :
52670
citations: 1
Reference
Hall D, Dai S, Tseng P, Malik Z, Nguyen M, Matt L, et al. Competition between ?-actinin and Ca²?-calmodulin controls surface retention of the L-type Ca²? channel Ca(V)1.2. Neuron. 2013;78:483-97 pubmed publisher
product information
Catalog Number :
52670
Product Name :
ACTN3-pEGFP
article :
doi10.1016/j.neuron.2013.02.032
id8164
pubmed_id23664615
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C3
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Full length human -actinin-3 was cloned via PCR into EcoR I/Sal I digested pEGFP-C3 using pcDNA3- -actinin-3 provided by M. Sheng (Genentech, CA) as template.
growth strain :
Overexpression of GFP-tagged -actinin-3
origin :
37
pi :
alt_names
actinin, alpha 3
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesACTN3D
geneACTN3
id89
genbank_ids
mutation
nameACTN3
shRNA_sequence
size2700
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
resistance markers :
1913
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA