p(HA)HIF1alpha (P402A,P564A) | Addgene 52636 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

p(HA)HIF1alpha (P402A,P564A)

catalog :

52636

citations: 4

Reference
Nelson J, Thin M, Evan T, Howell S, Wu M, Almeida B, et al. USP25 promotes pathological HIF-1-driven metabolic reprogramming and is a potential therapeutic target in pancreatic cancer. Nat Commun. 2022;13:2070 pubmed publisher
Hoffmann C, Mao X, Brown Clay J, Moreau F, Al Absi A, Wurzer H, et al. Hypoxia promotes breast cancer cell invasion through HIF-1?-mediated up-regulation of the invadopodial actin bundling protein CSRP2. Sci Rep. 2018;8:10191 pubmed publisher
Nanayakkara G, Alasmari A, Mouli S, Eldoumani H, Quindry J, McGinnis G, et al. Cardioprotective HIF-1Î±-frataxin signaling against ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 2015;309:H867-79 pubmed publisher
Kageyama Y, Koshiji M, To K, Tian Y, Ratcliffe P, Huang L. Leu-574 of human HIF-1alpha is a molecular determinant of prolyl hydroxylation. FASEB J. 2004;18:1028-30 pubmed

product information

Catalog Number :

52636

Product Name :

p(HA)HIF1alpha (P402A,P564A)

article :

doi	10.1096/fj.03-1233fje
id	8150
pubmed_id	15084514

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

Invitrogen

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

mammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutation

origin :

pi :

alt_names

HIF1A

hypoxia inducible factor 1, alpha subunit

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	KpnI
promoter	CMV
sequencing_primer_3	BGHrev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
gene	HIF1A
id	3091

genbank_ids

mutation

P402A, P564A

name

HIF1alpha P402A, P564A

shRNA_sequence

size

species

9606

Homo sapiens