This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pKF257
catalog :
52533
citations: 1
Reference
Böttcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, Philippou Massier J, et al. Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Res. 2014;42:e89 pubmed publisher
product information
Catalog Number :
52533
Product Name :
pKF257
article :
doi10.1093/nar/gku289
id8316
pubmed_id24748663
bacterial resistance :
Ampicillin
cloning :
backbonepCASPER5
backbone_mutation
backbone_origin
backbone_size10877
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth strain :
expresses I-SceI nuclease in Drosophila
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5KpnI
promotertubulin
sequencing_primer_3
sequencing_primer_5ctcgcacgcagtgccggctcaag
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesQ0160
geneSCEI
id854590
genbank_ids
mutation
nameI-SceI
shRNA_sequence
size831
species
4932
Saccharomyces cerevisiae
32644
Other
tags
locationN terminal on insert
tagNLS
locationN terminal on insert
tagHA
plasmid copy :
a fly strain containing I-SceI nuclease, obtained from Bloomington stock center
resistance markers :
1904
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA