This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
RGS-6xHis-BLMP-1-pcDNA3.1-
catalog :
52514
citations: 2
Reference
Chothe S, Jakka P, Boorla V, Ramasamy S, Gontu A, Nissly R, et al. Little Brown Bats (Myotis lucifugus) Support the Binding of SARS-CoV-2 Spike and Are Likely Susceptible to SARS-CoV-2 Infection. Viruses. 2023;15: pubmed publisher
Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, et al. DRE-1/FBXO11-dependent degradation of BLMP-1/BLIMP-1 governs C. elegans developmental timing and maturation. Dev Cell. 2014;28:697-710 pubmed publisher
product information
Catalog Number :
52514
Product Name :
RGS-6xHis-BLMP-1-pcDNA3.1-
article :
doi10.1016/j.devcel.2014.01.028
id8120
pubmed_id24613396
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1-
backbone_mutationaddded/inserted sequence encoding RGS-6xHis tag at start of MCS to allow for fusion protein production
backbone_originInvitrogen
backbone_size5421
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
expresses C. elegans BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminus
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR I
cloning_site_5Xba I
promoterCMV
sequencing_primer_3BGH-polyA reverse
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_F25D7.3
geneblmp-1
id172917
genbank_ids
NM_060322
mutationN75S mutation compared GenBank reference sequence NP_001251370.1
nameblmp-1
shRNA_sequence
size2454
species
6239
Caenorhabditis elegans
tags
locationN terminal on backbone
tag6xHis
plasmid copy :
insert cDNA was amplified from Open Biosystems Cat# OCE1182-7241376 (F25D7.3 C. elegans ORF Clone Caenorhabditis elegans pDONR201)
resistance markers :
2465
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA