This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
FLAG-HA-FbxO11- Fbox-pcDNA3.1-
catalog :
52502
citations: 1
Reference
Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, et al. DRE-1/FBXO11-dependent degradation of BLMP-1/BLIMP-1 governs C. elegans developmental timing and maturation. Dev Cell. 2014;28:697-710 pubmed publisher
product information
Catalog Number :
52502
Product Name :
FLAG-HA-FbxO11- Fbox-pcDNA3.1-
article :
doi10.1016/j.devcel.2014.01.028
id8120
pubmed_id24613396
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1-
backbone_mutationadded FLAG-HA tag in front of MCS, tag coding sequence has: Kozak FLAG - 5x Glycine HA 5x Glycine MCS .
backbone_originInvitrogen
backbone_size5439
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
expresses human FbxO11- Fbox in mammalian cells with FLAG-HA tag at N-terminus
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamH I
cloning_site_5Xba I
promoterCMV
sequencing_primer_3BGH-polyA reverse
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFBX11, IDDFBA, PRMT9, UBR6, UG063H01, VIT1
geneFBXO11
id80204
genbank_ids
BC136480
mutationdeletion of Fbox (deleted aa 71-116)
nameFbxO11
shRNA_sequence
size2400
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFLAG-HA
plasmid copy :
insert cDNA was amplified and modified from Open Biosystems Cat# MHS1768-99864123 (BC136480 Homo sapiens in pCR4-TOPO)
resistance markers :
2465
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA