Human Mevalonate Kinase (MK) | Addgene 52429 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Human Mevalonate Kinase (MK)

catalog :

52429

citations: 1

Reference
Potter D, Miziorko H. Identification of catalytic residues in human mevalonate kinase. J Biol Chem. 1997;272:25449-54 pubmed

product information

Catalog Number :

52429

Product Name :

Human Mevalonate Kinase (MK)

article :

doi	10.1074/jbc.272.41.25449
id	8094
pubmed_id	9325256

bacterial resistance :

Ampicillin

cloning :

backbone

pET-3d

backbone_mutation

backbone_origin

Novagen

backbone_size

4637

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

During PCR amplification, the codon for leucine 2 (TTG) was changed to valine (GTG). This single base mutation created an NcoI site (CCATGG) that overlapped the start codon of mevalonate kinase.

growth strain :

bacterial expression of human Mevalonate Kinase (MK)

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	NcoI
cloning_site_5	NcoI
promoter	T7
sequencing_primer_3	T7 terminal
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	LRBP, MK, MVLK, POROK3
gene	MVK
id	4598

genbank_ids

mutation

see comments

name

Human Mevalonate Kinase

shRNA_sequence

size

species

9606

Homo sapiens