This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCLC3sGFP
catalog :
52423
citations: 1
Reference
Li X, Wang T, Zhao Z, Weinman S. The ClC-3 chloride channel promotes acidification of lysosomes in CHO-K1 and Huh-7 cells. Am J Physiol Cell Physiol. 2002;282:C1483-91 pubmed
product information
Catalog Number :
52423
Product Name :
pCLC3sGFP
article :
doi10.1152/ajpcell.00504.2001
id8090
pubmed_id11997263
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutation
backbone_originClontech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Insert represents a portion of the 5 UTR and the full open reading frame of ClC-3 beginning at nucleotide 251 according to GenBank Accession number D17521.
growth strain :
mammalian expression of rat CLC3 short transcript fused to EGFP
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5XhoI
promoterCMV
sequencing_primer_3EGFP-N
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesClC-3
geneClcn3
id84360
genbank_ids
D17521
mutation
nameCLC3
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationC terminal on backbone
tagGFP
resistance markers :
1897
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA