pGL3-human OCT4 PE-SV40-Luc | Addgene 52415 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGL3-human OCT4 PE-SV40-Luc

catalog :

52415

citations: 4

Reference
Singh N, Padi S, Bearss J, Pandey R, Okumura K, Beltran H, et al. PIM protein kinases regulate the level of the long noncoding RNA H19 to control stem cell gene transcription and modulate tumor growth. Mol Oncol. 2020;14:974-990 pubmed publisher
Zimmerlin L, Park T, Huo J, Verma K, Pather S, Talbot C, et al. Tankyrase inhibition promotes a stable human naïve pluripotent state with improved functionality. Development. 2016;143:4368-4380 pubmed
Chen H, Aksoy I, Gonnot F, Osteil P, Aubry M, Hamela C, et al. Reinforcement of STAT3 activity reprogrammes human embryonic stem cells to naive-like pluripotency. Nat Commun. 2015;6:7095 pubmed publisher
Gafni O, Weinberger L, Mansour A, Manor Y, Chomsky E, Ben Yosef D, et al. Derivation of novel human ground state naive pluripotent stem cells. Nature. 2013;504:282-6 pubmed publisher

product information

Catalog Number :

52415

Product Name :

pGL3-human OCT4 PE-SV40-Luc

article :

doi	10.1038/nature12745
id	8075
pubmed_id	24172903

bacterial resistance :

Ampicillin

cloning :

backbone

pGL3 promoter

backbone_mutation

backbone_origin

Promega

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

Please note that there are several mismatches in between Addgene's quality control and the depositor's sequence. The mismatches do NOT affect plasmid function.

growth strain :

Luciferase reporter plasmid for PE OCT4 enhancer activity

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	Xho1
cloning_site_5	Kpn1
promoter
sequencing_primer_3	TCTTCCAGCGGATAGAATGG
sequencing_primer_5	CTCTCCATCAAAACAAAACGAA
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
gene	POU5F1
id	5460

genbank_ids

mutation

name

Proximal element of hOCT4 promoter

shRNA_sequence

size

1146

species

9606

Homo sapiens