This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTT5-hSDC1-2
catalog :
52378
citations: 1
| Reference |
|---|
Zhang Y, Wang N, Raab R, McKown R, Irwin J, Kwon I, et al. Targeting of heparanase-modified syndecan-1 by prosecretory mitogen lacritin requires conserved core GAGAL plus heparan and chondroitin sulfate as a novel hybrid binding site that enhances selectivity. J Biol Chem. 2013;288:12090-101 pubmed publisher
|
product information
Catalog Number :
52378
Product Name :
pTT5-hSDC1-2
article :
| doi | 10.1074/jbc.M112.422717 |
| id | 8067 |
| pubmed_id | 23504321 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pTT5 | |
| backbone_mutation | ||
| backbone_origin | NRC Biotechnology Research Institute | |
| backbone_size | 4401 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
Note that the last 3 amino acids of hSDC1, FYA , in the cytoplasmic region is an important PDZ domain binding site. This was not an issue in the associated JBC article, but please be aware of the difference. The truncation of hSDC1 also removed the native stop codon and a portion of the beta-globin polyA signal.
growth strain :
expresses human Syndecan-1 GAGAL replaced with GADEV of SDC2 in HEK293-EBNA1 (293E) suspension culture.
origin :
37
pi :
|
plasmid copy :
pTT5 backbone used in his plasmids was obtained from Yves Durocher at National Research Council of Canada (NRC)
resistance markers :
| 1540 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments