His Puc (aa 1-393) | Addgene 52295 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

His Puc (aa 1-393)

catalog :

52295

citations: 1

Reference
Zhuang M, Calabrese M, Liu J, Waddell M, Nourse A, Hammel M, et al. Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases. Mol Cell. 2009;36:39-50 pubmed publisher

product information

Catalog Number :

52295

Product Name :

His Puc (aa 1-393)

article :

doi	10.1016/j.molcel.2009.09.022
id	8044
pubmed_id	19818708

bacterial resistance :

Kanamycin

cloning :

backbone

pRSFDuet

backbone_mutation

backbone_origin

Novagen

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

When compared to GenBank reference sequence NP_524273.1, the Puc insert in this plasmid contains an additional 3 amino acids between aa 309 & 310. The numbering of Puc ending at aa 393 does not count the starting Met but does include the additional 3 amino acids.

growth strain :

Bacterial expression of His tagged Drosophila Puc fragment

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	unknown
cloning_site_5	unknown
promoter	T7
sequencing_primer_3
sequencing_primer_5	LacI-R
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG7850, 0238/03, 1351/08, CG7850, Dmel CG7850, JNK-DSP, PUC, PUC/MKP, Puc, hrt, l(3)84Eh, l(3)A251.1, l(3)j4E1, vco
gene	puc
id	40958

genbank_ids

mutation

name

Puc

shRNA_sequence

size

species

7227

Drosophila melanogaster