His-MBP human SPOP (aa 28-337) | Addgene 52294 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

His-MBP human SPOP (aa 28-337)

catalog :

52294

citations: 1

Reference
Zhuang M, Calabrese M, Liu J, Waddell M, Nourse A, Hammel M, et al. Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases. Mol Cell. 2009;36:39-50 pubmed publisher

product information

Catalog Number :

52294

Product Name :

His-MBP human SPOP (aa 28-337)

article :

doi	10.1016/j.molcel.2009.09.022
id	8044
pubmed_id	19818708

bacterial resistance :

Kanamycin

cloning :

backbone

pRSFDuet

backbone_mutation

backbone_origin

Novagen

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

SPOP-MATH-BTB+ were fused to the C terminus of His-tagged maltose-binding protein (His-MBP) and subcloned into the first cloning site of pRSFDuet vector (Novagen). TEV cleavage sites were engineered between the His-MBP tag and the fusion protein.

growth strain :

bacterial expression of human SPOP (aa 28-337) fused to His-MBP

origin :

pi :

alt_names

SPOP aa 28-337

SPOP MATH-BTB+

cloning

clone_method	Restriction Enzyme
cloning_site_3	unknown
cloning_site_5	unknown
promoter	T7
sequencing_primer_3
sequencing_primer_5	LacI-R
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BTBD32, NEDMACE, NEDMIDF, NSDVS1, NSDVS2, TEF2
gene	SPOP
id	8405

genbank_ids

mutation

name

SPOP

shRNA_sequence

size

species

9606

Homo sapiens