This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSC-PreE2
catalog :
52264
citations: 1
Reference
Weber A, Schuermann D, Schär P. Versatile recombinant SUMOylation system for the production of SUMO-modified protein. PLoS ONE. 2014;9:e102157 pubmed publisher
product information
Catalog Number :
52264
Product Name :
pSC-PreE2
article :
doi10.1371/journal.pone.0102157
id8797
pubmed_id25007328
bacterial resistance :
Ampicillin
cloning :
backbonepTXB3
backbone_mutationGyrA-intein and CBD domain replaced by expression unit with the PreScission cleavage site fused to Ubc9 and GST
backbone_originNew England Biolabs
backbone_size6707
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linker
growth temp :
In combination with a second vector for protein expression in BL21(DE3) cells, the selection pressure was reduced by half to 50 mg/L Ampicillin
origin :
37
pi :
alt_names
Ube2l
Ubc9
cloning
clone_methodRestriction Enzyme
cloning_site_3BglII
cloning_site_5XhoI
promoter
sequencing_primer_3TGCTAGTTATTGCTCAGCGG
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesC358B7.1, P18, UBC9
geneUBE2I
id7329
genbank_ids
NM_003336.1
mutation
nameUbc9 GST fusion
shRNA_sequence
size1773
species
9606
Homo sapiens
tags
locationC terminal on insert
tagGST
plasmid copy :
Ubc9 cDNAs was amplified from pGEX-based expression vector kindly gifted by Ronald Hay
resistance markers :
1922
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA