This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSA2
catalog :
52262
citations: 1
Reference
Weber A, Schuermann D, Schär P. Versatile recombinant SUMOylation system for the production of SUMO-modified protein. PLoS ONE. 2014;9:e102157 pubmed publisher
product information
Catalog Number :
52262
Product Name :
pSA2
article :
doi10.1371/journal.pone.0102157
id8797
pubmed_id25007328
bacterial resistance :
Streptomycin
cloning :
backbonepCDFDuet-1
backbone_mutationlinker with T7 terminator cloned into the AflII site between the two expression units of pCDFDuet1
backbone_originNovagen
backbone_size3781
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Bacterial expression of human 6His-tagged SUMO2 and SUMO-E1
growth temp :
In combination with a second vector for protein expression in BL21(DE3) cells, the selection pressure was reduced by half to 25 mg/L Streptomycin
origin :
37
pi :
alt_names
SMT3H2
cloning
clone_methodRestriction Enzyme
cloning_site_3PstI
cloning_site_5BglII
promoterT7
sequencing_primer_3
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesHSMT3, SMT3B, SMT3H2, SUMO3, Smt3A
geneSUMO2
id6613
genbank_ids
NM_006937.3
mutationC-terminal gly-gly
nameSUMO-2
shRNA_sequence
size330
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag6His-tag
alt_names
UBA 2
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5EcoRI
promoterT7
sequencing_primer_3
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesACCES, ARX, HRIHFB2115, SAE2
geneUBA2
id10054
genbank_ids
NM_005499.2
mutation
nameSAE-2
shRNA_sequence
size1947
species
9606
Homo sapiens
tags
alt_names
Aos1
Uble1a
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI
cloning_site_5NotI
promoterrbs
sequencing_primer_3
sequencing_primer_5GATGAGAAAGAGAATCTCAG
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesAOS1, HSPC140, SUA1, UBLE1A
geneSAE1
id10055
genbank_ids
NM_005500.2
mutation
nameSAE-1
shRNA_sequence
size1041
species
9606
Homo sapiens
tags
plasmid copy :
cDNAs of the SUMO components were amplified from pGEX-based expression vectors kindly gifted by Ronald Hay and Marc Hottiger
resistance markers :
1922
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA