This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSUMO1
catalog :
52258
citations: 7
Reference |
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product information
Catalog Number :
52258
Product Name :
pSUMO1
article :
doi | 10.1371/journal.pone.0102157 |
id | 8797 |
pubmed_id | 25007328 |
bacterial resistance :
Streptomycin
cloning :
backbone | pCDFDuet-1 | |
backbone_mutation | linker with T7 terminator cloned into the AflII site between the two expression units of pCDFDuet1 | |
backbone_origin | Novagen | |
backbone_size | 3781 | |
promoter | ||
sequencing_primer_3 | ||
sequencing_primer_5 | ||
vector_types |
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growth strain :
Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2
growth temp :
In combination with a second vector for protein expression in BL21(DE3) cells, the selection pressure was reduced by half to 25 mg/L Streptomycin
origin :
37
pi :
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plasmid copy :
cDNAs of the SUMO components were amplified from pGEX-based expression vectors kindly gifted by Ronald Hay and Marc Hottiger
resistance markers :
1922 |
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments