pFGC-pcoCas9 | Addgene 52256 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pFGC-pcoCas9

catalog :

52256

citations: 11

Reference
Nagy B, xd6 ktem A, Ferenc G, Ungor D, Kalac A, Kelemen Valkony I, et al. CRISPR/Cas9 Mutagenesis through Introducing a Nanoparticle Complex Made of a Cationic Polymer and Nucleic Acids into Maize Protoplasts. Int J Mol Sci. 2023;24: pubmed publisher
Fidan H, Calis O, Ari E, Atasayar A, Sarikaya P, Tek M, et al. Knockout of elF4E using CRISPR/Cas9 for large-scale production of resistant cucumber cultivar against WMV, ZYMV, and PRSV. Front Plant Sci. 2023;14:1143813 pubmed publisher
Ly D, Iqbal S, Fosu Nyarko J, Milroy S, Jones M. Multiplex CRISPR-Cas9 Gene-Editing Can Deliver Potato Cultivars with Reduced Browning and Acrylamide. Plants (Basel). 2023;12: pubmed publisher
G xf6 ritzer K, Grandits M, Gr xfc nwald Gruber C, Figl R, Mercx S, Navarre C, et al. Engineering the N-glycosylation pathway of Nicotiana tabacum for molecular pharming using CRISPR/Cas9. Front Plant Sci. 2022;13:1003065 pubmed publisher
Nayeri S, Baghban Kohnehrouz B, Ahmadikhah A, Mahna N. CRISPR/Cas9-mediated P-CR domain-specific engineering of CESA4 heterodimerization capacity alters cell wall architecture and improves saccharification efficiency in poplar. Plant Biotechnol J. 2022;20:1197-1212 pubmed publisher
Ng M, Ku Y, Yung W, Cheng S, Man C, Yang L, et al. MATE-Type Proteins Are Responsible for Isoflavone Transportation and Accumulation in Soybean Seeds. Int J Mol Sci. 2021;22: pubmed publisher
Wang X, Li M, Wong F, Luk C, Chung C, Yung W, et al. Increased copy number of gibberellin 2-oxidase 8 genes reduced trailing growth and shoot length during soybean domestication. Plant J. 2021;: pubmed publisher
Cui X, Balcerzak M, Schernthaner J, Babic V, Datla R, Brauer E, et al. An optimised CRISPR/Cas9 protocol to create targeted mutations in homoeologous genes and an efficient genotyping protocol to identify edited events in wheat. Plant Methods. 2019;15:119 pubmed publisher
Lee K, Zhu H, Yang B, Wang K. An Agrobacterium-Mediated CRISPR/Cas9 Platform for Genome Editing in Maize. Methods Mol Biol. 2019;1917:121-143 pubmed publisher
Cody W, Scholthof H, Mirkov T. Multiplexed Gene Editing and Protein Overexpression Using a Tobacco mosaic virus Viral Vector. Plant Physiol. 2017;175:23-35 pubmed publisher
Mercx S, Tollet J, Magy B, Navarre C, Boutry M. Gene Inactivation by CRISPR-Cas9 in Nicotiana tabacum BY-2 Suspension Cells. Front Plant Sci. 2016;7:40 pubmed publisher

product information

Catalog Number :

52256

Product Name :

pFGC-pcoCas9

article :

doi
id	8038
pubmed_id

bacterial resistance :

Kanamycin

cloning :

backbone

pFGC-RCS

backbone_mutation

expanded multiple cloning sites

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

CRISPR

Other

Plant expression

growth notes :

All labeled restriction sites (RS) in red on the map of the binary plasmid pFGC-pcoCas9 is single cut except StuI, which was used to insert the 35SPPDK:pcoCas9:NOS expression cassette. Among those RSs, EcoRI, XhoI, AscI, PacI, AsiSI, SbfI, and XmaI/SmaI can be used to insert single or multiple guide RNA expression cassettes (AscI, PacI, and SbfI are strongly recommended). The pUC119-gRNA (Addgene plasmid #52255) construct is used as the PCR template to assemble a new guide RNA expression cassette flanked by desired restriction site(s) according to Fig. S5 in Li et al. Nat. Biotechnol. 31: 688-691.

growth strain :

A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA

origin :

pi :

alt_names

Cas9

plant codon optimized Cas9

cloning

clone_method	Restriction Enzyme
cloning_site_3	StuI
cloning_site_5	StuI
promoter	constitutive 35SPPDK promoter
sequencing_primer_3	Sequencing primer for 35SPPDK:pcoCas9:NOS (from somewhere close to the 35SPPDK promoter): 5 AGTTGGGTAACGCCAGGG 3
sequencing_primer_5	Sequencing primer for multiple cloning sites (from somewhere close to the EcoRI site): 5 AATAAAAACTGACTCGGA 3
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

35SPPDK:pcoCas9:NOS cassette

shRNA_sequence

size

species

100

Synthetic